Abstract: Inhibitors of HIV membrane fusion and a method of identifying drugs or agents which inhibit binding of the N-helix coiled-coil and the C helix of HIV gp41 envelope protein.

Abstract: A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed.

Abstract: Novel methods of reproducibly determining a limited population of polymorphisms are disclosed.

Abstract: The present invention relates to a method of delivering a moiety of interest into a cell comprising contacting the cell with a complex comprising the moiety of interest covalently linked to a heat shock protein, under conditions appropriate for entry of the complex into the cell. The invention also relates to a method of delivering a moiety of interest into a cell capable of taking up a complex comprising the moiety of interest covalently linked to a heat shock protein comprising contacting the cell with a complex comprising the moiety of interest covalently linked to a heat shock protein, under conditions appropriate for entry of the complex into the cell. Also encompassed by the present invention is a method of delivering a moiety of interest into an antigen presenting cell comprising contacting the cell with a complex comprising the moiety of interest covalently linked to a heat shock protein, under conditions appropriate for entry of the complex into the cell.

Abstract: A method of producing mutant/targeted non-human mammals, such as mutant mice that does not require production of chimera and permits the introduction of multiple mutations in embryos and, thus, avoids the necessity of breeding to combine all of the desired mutations in a single animal. The method is efficient in producing ES mice.

Abstract: Described herein are chimeric peptides comprising a soluble trimeric coiled-coil and all or a portion of the N-peptide region of HIV gp41. These molecules are stable, trimeric coiled-coils that inhibit HIV entry into cells, such as human cells. Such peptides can be further assessed to demonstrate their ability to serve as potent anti-HIV therapeutic molecules and thus, as therapeutic molecules or drugs.

Abstract: Novel alterations in the glycerol kinase gene are described. Also described are methods of predicting or assisting in the prediction of impaired glucose tolerance and type 2 diabetes mellitus.

Abstract: Systems and methods for across platform and multiple dataset classification. In one embodiment the systems combine a Large Bayes classification framework, constructed from discovered itemsets or common patterns of data, with a definition of combined relative features to represent the original values. One realization of this method is that different datasets representing the same biological system display some amount of invariant biological characteristics independent of the idiosyncrasies of sample sources, preparation and the technological platform used to obtain the measurements.

Abstract: The invention provides nucleic acid segments of the human genome, particularly nucleic acid segments from a gene, including polymorphic sites. Allele-specific primers and probes hybridizing to regions flanking or containing these sites are also provided. The nucleic acids, primers and probes are used in applications such as phenotype correlations, forensics, paternity testing, medicine and genetic analysis. A role for the thrombospondin gene(s) in vascular disease is also disclosed. Use of single nucleotide polymorphisms in the thrombospondin gene(s) for diagnosis, prediction of clinical course and treatment response, development of therapeutics and development of cell-culture-based and animal models for research and treatment are disclosed.

Abstract: The present invention relates to isolated raptor nucleic acid molecules of mammalian origin (e.g., human) and complements, portions and variants thereof. Another aspect of the invention are isolated raptor polypeptides of mammalian origin and portions thereof, and antibodies or antigen binding fragments thereof that specifically bind a raptor polypeptide. The present invention also relates to constructs and host cells comprising the nucleic acid molecules described herein. In addition, the present invention relates to uses of the nucleic acid and polypeptide molecules provided herein.

Abstract: Engineered mRNA useful in producing libraries of engineered mRNAs, polypeptide-engineered mRNA conjugates and diverse encoded polypeptide libraries, as well as novel ribozymes that join an mRNA to the translation product of the mRNA and methods of identifying members of diverse encoded polypeptide libraries which exhibit a desired activity. Also described are polypeptide-nucleic acid tag conjugates, methods of producing the conjugates and uses therefor.

Abstract: Described herein are chimeric peptides comprising a soluble trimeric coiled-coil and all or a portion of the N-peptide region of HIV gp41. These molecules are stable, trimeric coiled-coils that inhibit HIV entry into cells, such as human cells. Such peptides can be further assessed to demonstrate their ability to serve as potent anti-HIV therapeutic molecules and thus, as therapeutic molecules or drugs.

Abstract: Recombinant pox viruses capable of expressing cell-encoded, tumor-associated antigens are disclosed. The recombinant viruses are useful for evoking an immune response against the antigen.

Abstract: Distinct gene expression programs activated in response to different pathogens in macrophages are disclosed. Methods of diagnosis and treatment are also disclosed.

Abstract: A method of producing a non-human mammalian embryo, such as a mouse embryo, by nuclear cloning, in which the nucleus from a non-human mammalian embryonic stem (ES) cell (e.g., a non-human mammalian F1 ES cell), such as the nucleus of a mouse F1 ES cell, is introduced into an enucleated non-human mammalian oocyte, such as an enucleated mouse oocyte; embryos produced by the method; a method of producing mice from the resulting embryos and the mice produced thereby.

Abstract: Disclosed are tRNA analogues which comprise a tRNA, such as tRNAphe; a nonstandard amino acid moiety which acts as an acceptor substrate, but not as a donor substrate, for ribosome-directed nonstandard polymer transfer and, thus, is stably linked to the acceptor stem of the tRNA; and a reactive or activatible moiety near or within the anticodon stem loop of the tRNA that can medidate the covalent coupling of the tRNA analogue to mRNA. Also disclosed are nonstandard polymer-tRNA analogue-mRNA fusions; libraries of encoded nonstandard polymers; methods of producing and screening the libraries; and target members and their uses.

Abstract: The invention provides nucleic acid segments of the human genome, particularly necleic acid segments from the coding region of a gene, including polymorphic sites. Allele-specific primers and probes hybridizing to regions flanking or containing these sites are also provided. The nucleic acids, primers and probes are used in applications such as phenotype correlations, forensics, paternity testing, medicine and genetic analysis.

Abstract: The invention involves high throughput methods for identifying properties of cells under a variety of cellular conditions. The high throughput methods have a variety of uses, including methods for identifying cellular modulators such as pharmacological agents or environmental conditions, methods for identifying a cellular phenotype and methods for identifying novel genes.

Abstract: A neural network system and method for analyzing data sets, especially microarray gene expression data. The neural network is trained to generate time-dependent outcome predictions based on input features and outcome functions for a number of subjects. The features may be highly dimensional relative to the number of subjects, and feature selection is applied to the input feature data for training the neural network. A trained neural network processes input features from a subject to generate an outcome function that reflects the probability of the occurrence of an event at a given time point for that subject.

Abstract: Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate.