Patents Assigned to Wilson Wolf Manufacturing
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Patent number: 11963979Abstract: An in vitro expansion process for rapid expansion of antigen specific T cells, such as allogeneic antigen specific T cells comprising the steps culturing in a gas permeable vessel a population of PBMCs (such as allogeneic PBMCs) in the presence of antigen, for example a peptide or peptide mix relevant to a target antigen(s), in the presence of an exogenous cytokine characterized in that the expansion to provide the desired population of T cells is 14 days or less, for example 9, 10, 11 or 12 days, such as 10 days. The disclosure also extends to T cell populations generated by and obtained from the method and the use of same in therapy.Type: GrantFiled: June 22, 2018Date of Patent: April 23, 2024Assignees: AlloVir, Inc., Baylor College of Medicine, Wilson Wolf Manufacturing CorporationInventors: Rainer Ludwig Knaus, Katy Rebecca Newton, Juan Vera, Ann Leen, Cliona Rooney, John R. Wilson
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Patent number: 11891595Abstract: Improved cell culture devices and related methods that overcome the limitations of prior devices and methods, by creating devices that can integrate a variety of novel attributes. These various attributes include the use of gas permeable material and medium volumes that exceed conventional devices as well as compartments that can facilitate the long term study of high density cultures with reduced disruption of the culture environment, the ability to study the migration of items of interest including substances such as chemokine, track the movement of cells, and monitor cell to cell interactions.Type: GrantFiled: March 24, 2023Date of Patent: February 6, 2024Assignee: Wilson Wolf Manufacturing LLCInventors: John R. Wilson, Daniel P. Welch
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Patent number: 11873474Abstract: Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.Type: GrantFiled: September 16, 2021Date of Patent: January 16, 2024Assignee: Wilson Wolf ManufacturingInventors: Daniel P. Welch, John R. Wilson
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Patent number: 11821002Abstract: An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.Type: GrantFiled: March 7, 2022Date of Patent: November 21, 2023Assignees: Baylor College of Medicine, Wilson Wolf ManufacturingInventors: Juan F. Vera, Cliona M. Rooney, Ann M. Leen, John R. Wilson
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Patent number: 11613725Abstract: Improved cell culture devices and related methods that overcome the limitations of prior devices and methods, by creating devices that can integrate a variety of novel attributes. These various attributes include the use of gas permeable material and medium volumes that exceed conventional devices as well as compartments that can facilitate the long term study of high density cultures with reduced disruption of the culture environment, the ability to study the migration of items of interest including substances such as chemokine, track the movement of cells, and monitor cell to cell interactions.Type: GrantFiled: May 9, 2016Date of Patent: March 28, 2023Assignee: Wilson Wolf ManufacturingInventors: John R. Wilson, Daniel P. Welch
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Patent number: 11401503Abstract: An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.Type: GrantFiled: December 6, 2018Date of Patent: August 2, 2022Assignees: The United States of America, as represented by the Secretary, Department of Health and Human Services, Wilson Wolf Manufacturing CorporationInventors: Steven A. Rosenberg, Mark E. Dudley, David Stroncek, Marianna Sabatino, Jianjian Jin, Robert Somerville, John R. Wilson
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Patent number: 11377635Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: GrantFiled: July 7, 2017Date of Patent: July 5, 2022Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Patent number: 11268066Abstract: An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.Type: GrantFiled: November 26, 2019Date of Patent: March 8, 2022Assignees: Wilson Wolf Manufacturing, Baylor College of MedicineInventors: Juan F. Vera, Cliona M. Rooney, Ann M. Leen, John R. Wilson
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Publication number: 20220049215Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: ApplicationFiled: November 2, 2021Publication date: February 17, 2022Applicant: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Patent number: 11155778Abstract: Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.Type: GrantFiled: October 5, 2017Date of Patent: October 26, 2021Assignee: Wilson Wolf ManufacturingInventors: Daniel P. Welch, John R. Wilson
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Publication number: 20210253997Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: ApplicationFiled: February 2, 2021Publication date: August 19, 2021Applicant: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Patent number: 10584354Abstract: The invention relates to improved methods of genetically modifying animal cells by decreasing the distance between cells and genetic modification agents in order to increase the number of cells modified by a given quantity of genetic modification agents and/or reduce the quantity of genetic modification agents needed to transduce a given number of cells.Type: GrantFiled: September 23, 2014Date of Patent: March 10, 2020Assignee: Wilson Wolf ManufacturingInventor: John R. Wilson
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Patent number: 10533156Abstract: An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.Type: GrantFiled: December 30, 2016Date of Patent: January 14, 2020Assignees: Baylor College of Medicine, Wilson Wolf ManufacturingInventors: Juan F. Vera, Cliona M. Rooney, Ann M. Leen, John R. Wilson
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Patent number: 10385316Abstract: The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and/or that employ a particular bioreactor having a gas permeable membrane.Type: GrantFiled: August 24, 2016Date of Patent: August 20, 2019Assignees: Baylor College of Medicine, Wilson Wolf ManufacturingInventors: Ann Marie Leen, Ulrike Gerdemann, Cliona M. Rooney, Juan F. Vera Valdes, John R. Wilson
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Patent number: 9963677Abstract: The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and/or that employ a particular bioreactor having a gas permeable membrane.Type: GrantFiled: August 24, 2010Date of Patent: May 8, 2018Assignees: Baylor College of Medicine, Wilson Wolf ManufacturingInventors: Ann M. Leen, Ulrike Gerdemann, Cliona M. Rooney, Juan Vera, John R. Wilson
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Patent number: 9840692Abstract: Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.Type: GrantFiled: June 24, 2014Date of Patent: December 12, 2017Assignee: Wilson Wolf ManufacturingInventors: Daniel P. Welch, John R. Wilson
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Patent number: 9732317Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: GrantFiled: July 2, 2014Date of Patent: August 15, 2017Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Publication number: 20170152478Abstract: An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.Type: ApplicationFiled: December 12, 2016Publication date: June 1, 2017Applicants: The USA, as represented by the Secretary, Department of Health and Human Service, Wilson Wolf Manufacturing CorporationInventors: Steven A. Rosenberg, Mark E. Dudley, David Stroncek, Marianna Sabatino, Jianjian Jin, Robert Somerville, John R. Wilson
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Patent number: RE48445Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: June 12, 2018Date of Patent: February 23, 2021Assignee: Wilson Wolf ManufacturingInventors: John R. Wilson, Douglas A. Page, Daniel Welch, Alison Robeck
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Patent number: RE49293Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: June 12, 2018Date of Patent: November 15, 2022Assignee: Wilson Wolf ManufacturingInventors: John R. Wilson, Douglas A. Page, Daniel Welch, Alison Robeck