Patents Assigned to Wilson Wolf Manufacturing Corporation
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Patent number: 11963979Abstract: An in vitro expansion process for rapid expansion of antigen specific T cells, such as allogeneic antigen specific T cells comprising the steps culturing in a gas permeable vessel a population of PBMCs (such as allogeneic PBMCs) in the presence of antigen, for example a peptide or peptide mix relevant to a target antigen(s), in the presence of an exogenous cytokine characterized in that the expansion to provide the desired population of T cells is 14 days or less, for example 9, 10, 11 or 12 days, such as 10 days. The disclosure also extends to T cell populations generated by and obtained from the method and the use of same in therapy.Type: GrantFiled: June 22, 2018Date of Patent: April 23, 2024Assignees: AlloVir, Inc., Baylor College of Medicine, Wilson Wolf Manufacturing CorporationInventors: Rainer Ludwig Knaus, Katy Rebecca Newton, Juan Vera, Ann Leen, Cliona Rooney, John R. Wilson
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Patent number: 11401503Abstract: An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.Type: GrantFiled: December 6, 2018Date of Patent: August 2, 2022Assignees: The United States of America, as represented by the Secretary, Department of Health and Human Services, Wilson Wolf Manufacturing CorporationInventors: Steven A. Rosenberg, Mark E. Dudley, David Stroncek, Marianna Sabatino, Jianjian Jin, Robert Somerville, John R. Wilson
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Patent number: 11377635Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: GrantFiled: July 7, 2017Date of Patent: July 5, 2022Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Publication number: 20220049215Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: ApplicationFiled: November 2, 2021Publication date: February 17, 2022Applicant: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Publication number: 20210253997Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: ApplicationFiled: February 2, 2021Publication date: August 19, 2021Applicant: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Patent number: 9732317Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: GrantFiled: July 2, 2014Date of Patent: August 15, 2017Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Publication number: 20170152478Abstract: An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.Type: ApplicationFiled: December 12, 2016Publication date: June 1, 2017Applicants: The USA, as represented by the Secretary, Department of Health and Human Service, Wilson Wolf Manufacturing CorporationInventors: Steven A. Rosenberg, Mark E. Dudley, David Stroncek, Marianna Sabatino, Jianjian Jin, Robert Somerville, John R. Wilson
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Patent number: 9255243Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: October 8, 2004Date of Patent: February 9, 2016Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
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Patent number: 8809044Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.Type: GrantFiled: December 7, 2007Date of Patent: August 19, 2014Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Patent number: 8697443Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: April 2, 2010Date of Patent: April 15, 2014Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Douglas A. Page, Daniel P. Welch, Alison Robeck
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Patent number: 8518692Abstract: Gas permeable devices and methods are disclosed that provide highly efficient cell culture. Gas compartments, made up at least in part of gas permeable material, are dispersed within the culture device in locations that allow cells to remain within a fixed distance from a gas transmission location as the device scales in the horizontal direction. Gas permeable walls of the gas compartment(s) allow gas exchange with the ambient gas. Such an arrangement provides many advantages including the ability to eliminate the need for a gas-liquid interface, allow cell culture to proceed in the static mode (i.e. absent the need for media or gas to be pumped through the device), allow the scale of the device to increase in both the horizontal direction and vertical direction, reduce the rate of media evaporation, allow uncomplicated and low cost device fabrication, and provide the capacity for reduced feeding frequency.Type: GrantFiled: July 8, 2009Date of Patent: August 27, 2013Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Publication number: 20120244133Abstract: An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.Type: ApplicationFiled: March 20, 2012Publication date: September 27, 2012Applicants: Wilson Wolf Manufacturing Corporation, Human ServiceInventors: Steven A. Rosenberg, Mark E. Dudley, David Stroncek, Marianna Sabatino, Jianjian Jin, Robert Somerville, John R. Wilson
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Patent number: 8158426Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: July 29, 2011Date of Patent: April 17, 2012Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
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Patent number: 8158427Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.Type: GrantFiled: July 29, 2011Date of Patent: April 17, 2012Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
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Patent number: 7799560Abstract: A versatile compartmentalized cell culture device, with a selectively permeable membrane separating the compartments, provides many attributes relative to traditional devices. It can be configured for high-density cell culture, co-culture, and sample dialysis while rolling or standing still. It can also be configured for continuous movement of liquid between compartments. The wide combination of attributes not found in other membrane based cell culture and bioprocessing devices includes more cell capacity, more cell secreted product capacity, higher cell and product density, increased medium capacity, minimized use of exogenous growth factors, compatibility with standard cell culture equipment and protocols, increased scale up efficiency, capacity to function when rolling or standing still, capacity for perfusion without the need for pumps, and more efficient sample dialysis.Type: GrantFiled: November 10, 2004Date of Patent: September 21, 2010Assignee: Wilson Wolf Manufacturing CorporationInventors: John R. Wilson, Daniel P. Welch, Alison Robeck, Douglas A. Page
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Patent number: 7229820Abstract: A disclosure is made of various apparatus and methods for culturing and preserving cells and tissue in ways that minimize contamination potential, direct cells to reside in desired areas, allow uniform cell distribution during seeding, provide optimal growth conditions by controlling the amount of medium residing in proximity of cells, allow desired compounds and molecules to reside in proximity of the cells, allow co-culture, provide for efficient scale up, allow a desired shape of tissue to be created while retaining a closed system, and allow cryopreservation and reconstitution of cell and tissue while retaining a closed system. The apparatus and methods can be combined to prevent the need to remove the tissue from the enclosure at any point during the sterilization, seeding, culturing, cryopreservation, shipping, or restoration process. Also disclosed is an apparatus and method of pipette interface with a container in a manner that blocks contaminants from entering the container.Type: GrantFiled: June 13, 2003Date of Patent: June 12, 2007Assignee: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson
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Publication number: 20040043481Abstract: A disclosure is made of various apparatus and methods for culturing and preserving cells and tissue in ways that minimize contamination potential, direct cells to reside in desired areas, allow uniform cell distribution during seeding, provide optimal growth conditions by controlling the amount of medium residing in proximity of cells, allow desired compounds and molecules to reside in proximity of the cells, allow co-culture, provide for efficient scale up, allow a desired shape of tissue to be created while retaining a closed system, and allow cryopreservation and reconstitution of cell and tissue while retaining a closed system. The apparatus and methods can be combined to prevent the need to remove the tissue from the enclosure at any point during the sterilization, seeding, culturing, cryopreservation, shipping, or restoration process. Also disclosed is an apparatus and method of pipette interface with a container in a manner that blocks contaminants from entering the container.Type: ApplicationFiled: June 13, 2003Publication date: March 4, 2004Applicant: Wilson Wolf Manufacturing CorporationInventor: John R. Wilson