Abstract: A method for enriching or isolating tumor specific MILs is described. This method includes the steps of preparing MILs from the bone marrow of a cancer patient; evaluating the MILs for gene expression, metabolic profile, or phenotype; and selecting and isolating the MILs that exhibit the gene expression, metabolic profile, or phenotype. Compositions containing the MILs and methods of treating cancer with the enriched MILs are also described.

Abstract: Marrow-infiltrating lymphocytes (“MILs”) comprising a chimeric antigen receptor (“CAR”) are provided. In some aspects, the embodiments relate to a method for making a recombinant MIL, comprising obtaining bone marrow comprising MILs; and transfecting, transforming, or transducing the MILs with a nucleic acid encoding a chimeric antigen receptor, resulting in a CAR-MIL.

Abstract: Lung cancer specific marrow infiltrating lymphocytes (“MILs”) and methods for making and using the same are described. Disclosed herein is a method for treating a subject having lung cancer with marrow infiltrating lymphocytes, the method comprising the steps of: (a) culturing a bone marrow sample obtained from the subject having lung cancer with an anti-CD3 antibody and an anti-CD28 antibody in a hypoxic environment to produce hypoxic-activated marrow infiltrating lymphocytes; (b) culturing the hypoxic-activated marrow infiltrating lymphocytes in a normoxic environment to produce the therapeutic activated marrow infiltrating lymphocytes; and (c) administering the therapeutic activated marrow infiltrating lymphocytes to the subject having lung cancer.

Abstract: The disclosure provides for compounds comprising cancer specific marrow infiltrating lymphocytes and methods for making and using the same.

Abstract: Marrow-infiltrating lymphocytes (“MILs”) comprising a chimeric antigen receptor (“CAR”) are provided. In some aspects, the embodiments relate to a method for making a recombinant MIL, comprising obtaining bone marrow comprising MILs; and transfecting, transforming, or transducing the MILs with a nucleic acid encoding a chimeric antigen receptor, resulting in a CAR-MIL. In some aspects, the embodiments relate to a method for treating a condition in a subject, comprising administering to the subject a MIL comprising a CAR. In some aspects, the condition is cancer, such as prostate cancer.

Abstract: The disclosure provides for compounds comprising prostate cancer specific marrow infiltrating lymphocytes and methods for making and using the same.

Abstract: The present invention relates to the field of cancer therapy. More specifically, the present invention provides methods and compositions useful for augmenting anti-tumor immunity. In one embodiment, a method for treating or preventing post-allogeneic transplant relapse in a subject who has received post-transplant cyclophosphamide treatment comprises the steps of (a) obtaining a bone marrow sample from the subject; (b) expanding the marrow infiltrating lymphocytes (MILs) present in the sample; and (c) administering the MILs to the subject. In a specific embodiment, the method significantly reduces the likelihood of developing GVHD.

Abstract: Embodiments provided for herein relate to compositions and methods related to chimeric proteins that can be used to modulate a subject's immune system and, for example, treat cancer.