Abstract: Methods of detecting cancer or a particular type or subtype thereof in a subject and treating the cancer or particular type or subtype thereof. The detection can comprise determining fragmentation patterns of classifier cell-free deoxyribonucleic acid (cfDNA) from the subject and classifying the fragmentation patterns to identify the subject as being negative or positive for the cancer or the particular type or subtype thereof. The classifier cfDNA can comprise cfDNA corresponding to at least a portion of at least one exon of one or more classifier genes. The exon can comprise the first exons of the classifier genes. The treating can comprise the specific type of subtype of cancer that is detected.

Abstract: Described herein is cancer biomarker, e.g., a vesicle, capture surface that includes a substrate, a first plurality of nanoparticles attached to the substrate; a plurality of bifunctional tethers, wherein a first functionality of the bifunctional tethers is attached to at least a portion of the first plurality of nanoparticles; a second plurality of nanoparticles attached to a second functionality of at least a portion of the plurality of bifunctional tethers; a capture agent attached to at least a portion of the second plurality of nanoparticles; and a plurality of polymer brush molecules attached to the surface, wherein the polymer brush molecules have a lower molecular weight than the bifunctional tethers, and wherein the polymer brush molecules reduce nonspecific binding to the surface. Also described are method of capturing cancer biomarkers such as vesicles from a liquid biopsy sample.

Abstract: Aspects of the disclosure are directed to additively manufacturing a three-dimensional structure. As may be implemented in accordance with one or more embodiments, a plurality of stacked layers are deposited, and for one or more respective layers of the plurality of stacked layers, pores are formed within the layer by applying pulsed energy to the layer. The pulsed energy is used to create a space sealed within the layer and having an inner surface defined by material of the layer.

Abstract: Genetically modified CCNT1 and XPO1 genes encoding proteins that inhibit virus infection in cells. The genetically modified CCNT1 gene encodes a protein with a C261Y substitution with respect to the human CCNT1 protein. The genetically modified XPO1 gene encodes a protein with P411T, M412V, and/or F414S substitutions with respect to the human XPO1 protein. The genetically modified CCNT1 and XPO1 genes can be introduced in cells. The cells comprising the genetically modified CCNT1 and XPO1 genes can be introduced in a subject with a virus infection to treat the infection.

Abstract: Tandem electrodialysis (ED) cell systems and methods for using the tandem ED cell systems to extract and recover ions from ion-containing solutions are provided. The tandem ED cell systems are composed of ion-extraction and ion-recovery ED cells. A redox couple contained in the anolyte of the ion-extraction ED cell is different from a redox couple contained in the catholyte of the ion-extraction ED cell. The electrode reactions in the ion-extraction ED cell are reversed in the ion-recovery ED cell, with the anolyte and catholyte of the two ED cells swapped and continuously circulated. As a result, the redox species in the anolyte and catholyte of the two cells are never depleted, which allows for achieving ion extraction and ion recovery with the use of a minimal amount of the redox couples.

Abstract: Systems, methods, compositions of matter, and kits for undermedia repellency are disclosed. In some cases, these involve a first volume of a first liquid presented in a second volume of a second liquid above a first location of a first surface. The first liquid, second liquid, and first location can have properties sufficient to give rise to undermedia perfect liquid repellency.

Abstract: An expression cassette for gene silencing and replacement, including, in operable communication, a promoter, an expression attenuator, a nucleotide sequence encoding a gene for a replacement target protein, and an shRNA sequence for knockdown of an endogenous variant of the target protein, wherein the promoter, the expression attenuator, the nucleotide sequence encoding the gene for the replacement target protein, and the shRNA are expressed as a single transcript. Also included are expression vectors and cells. Also included are methods of silencing and replacement of a target gene in a cell in culture by transforming the cells with the expression vectors described herein. Also included are minimal expression cassettes suitable for therapeutic methods.

Abstract: Mycobacterial-specific biomarkers and methods of using such biomarkers for diagnosis of mycobacterial infection in a mammal are disclosed.

Abstract: Provided herein are dynamic adaptive scheduling (DAS) systems. In some embodiments, the DAS systems include a first scheduler, a second scheduler that is slower than the first scheduler, and a runtime preselection classifier that is operably connected to the first scheduler and the second scheduler, which runtime preselection classifier is configured to effect selective use of the first scheduler or the second scheduler to perform a given scheduling task. Related systems, computer readable media, and additional methods are also provided.

Abstract: The present technology relates to compositions and methods for modulating expression of genes which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and -B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.

Abstract: Aspects of this disclosure are directed to methods and apparatuses involving the characterization of livestock feeding functions. As may be implemented as or with one or more embodiments herein, networked cameras are configured to capture images of a livestock feed area and machine-vision logic circuitry characterizes, based on the captured images, an amount of available feed and the presence of livestock in the livestock feed area depicted in the captured image over time. Feed-control logic circuitry may assign time-based condition values each respective feed area characterized by the cameras based on the characterized amount of available feed and the characterized presence of livestock provided via the machine-vision logic circuitry. An instruction characterizing the presentation of feed in the feed area may be output based on the assigned time-based condition values and a current time. Such an output may be used to control the presentation amount, timing and/or other feeding characteristics.

Abstract: Methods of generating phenocopy signatures and uses thereof. The methods of generating phenocopy signatures can include determining gene expression signatures that predict the presence of mutations in training cells. Uses of the phenocopy signatures include identifying cells exhibiting the phenocopy signatures, identifying subjects comprising cells that exhibit the phenocopy signatures, methods of using the phenocopy signatures to predict cells sensitive to treatment with drugs, methods of treating cells with phenocopy signatures predicted to be sensitive to treatment with drugs, methods of using phenocopy signatures to predict subjects sensitive to treatment with drugs, and methods of treating subjects with phenocopy signatures predicted to be sensitive to treatment with drugs.

Abstract: Methods of generating linear regression predictor models capable of predicting responses of patients afflicted with diseases to treatments, methods of using the linear regression predictor models to predict the responses of the patients to the treatments, and methods of administering the treatments to the patients.

Abstract: This invention provides minimally invasive methods, reagents, diagnostic and prognostic markers useful for staging, prognosis and surveillance of colorectal cancers, and therapeutic intervention in individuals with colorectal cancers, or individuals who may be susceptible to developing colorectal cancers.

Abstract: In accordance with some embodiments, systems, methods, and media for improving signal-to-noise ratio in single-photon data are provided. In some embodiments, a system comprises: a processor programmed to: generate, for each of a plurality of pixel locations, initial photon flux estimates based on a first set of photon transients including photon transients associated with the pixel location and neighboring pixel locations, each of the photon transients comprises a histogram of photon counts; identify, for a scene patch associated with each of the plurality of pixel locations, one or more similar scene patches using intensity information for each of the plurality of pixel locations; and generate, for each of the plurality of pixel locations, final photon flux estimates based on a second set of photon transients including photon transients associated with the scene patch and each of the one or more similar scene patches.

Abstract: Regulatory elements that drive Schwann cell-specific gene expression, nucleic acid vectors, virus particles, and therapeutic compositions incorporating these constructs; and methods of using these various compositions to alter gene expression in a Schwann cell or to treat a subject having a condition associated with misexpression or insufficient function of a target gene in Schwann cells.

Abstract: A system for monitoring organ health during treatment for cancer and the like makes use of physiological imaging of the kind used for treatment monitoring and organ-specific processing to provide a comprehensive assessment of treatment side-effects.

Abstract: Provided is a process for manufacturing magnetically tunable nano-resonators. The nano-resonators comprise nanoparticles of a crystalline magnetic material embedded into cavities of a substrate.

Abstract: A sample extraction cassette and method are provided to test for a target in a sample. The sample is obtained on a swab. The swab is inserted into a chamber in a case and into contact with a contact portion of a membrane. The contact portion of the membrane is axially moved into sequential communication with a wash fluid and a reaction fluid. The reaction fluid reacts with the target to provide a visual display corresponding to the presence of the target.

Abstract: A divertor for system supporting plasma for fusion employs permanent magnets providing far weaker magnetic field strength than conventionally used in cryogenic magnetic systems through an ability to place the permanent magnets in close proximity to the plasma containment volume.