Abstract: The present technology provides disulfide-containing lipopeptides of Formula I. Such lipids may be incorporated into lipid nanoparticles for delivery of nucleic acids, nucleotides (e.g., NAD+ or NADH), polypeptides (including proteins), and complexes of proteins and nucleic acids, e.g., Cas9 RNP. The present technology also provides methods for delivering the nanoparticles to a cell and methods of treating a condition or disorder using the lipid nanoparticles.

Abstract: Systems, computer-readable medium, methods and apparatus, and/or devices to causally quantify the potentially heterogeneous direct effect of a marketing program on focal individuals (e.g., egos) and the indirect effect on those connected to the focal ones (e.g., alters). A primary data structure (focal individual or ego) may be connected to multiple secondary data structures (alters) by a primary-to-secondary linkage net. Moreover, different secondary data structures may be connected together by a secondary-to-secondary linkage net, and some secondary data structures maybe connected to multiple primary data structures. Thus, in a complicated arrangement of connections, an input change directed at a single primary data structure may have both direct effects thereon, and complex indirect effects on secondary data structures. The systems, computer-readable medium, methods and apparatus, and/or devices provide mechanisms to evaluate these effects.

Abstract: One or more computing devices, systems, and/or methods are provided. In an example, a method includes tuning a sensor comprising a first resonator having an effective gain, a second resonator having an effective loss, and a coupling circuit connected between the first resonator and the second resonator at an extrema degeneracy point with a nonlinear response to a force applied to the sensor. At least one of the first resonator, the second resonator, or the coupling circuit comprises a variable capacitor having a capacitance that varies as a function of the force. The method comprises injecting an input signal into one of the first resonator or the second resonator, receiving an output signal from one of the first resonator or the second resonator, and determining a magnitude of a force based on a difference between a first frequency extrema point and a second frequency extrema point in the output signal.

Abstract: A method (and corresponding apparatus) of analyzing the behavior of an electrochemical double layer (EDL) by replacing a classic capacitor in an astable multivibrator circuit with an electrochemical cell. By pushing the EDL into resonance, the induced charging and discharging of the double layer results in a square waveform output that has a characteristic frequency audible to the human ear. Variations in the electrolyte concentration and identity yield distinct frequencies. The method provides sensory insight into EDL rearrangement and behavior with an apparatus that is simple and robust. The apparatus allows the analysis of time-dependent EDL rearrangements occurring upon the application of a voltage. The apparatus provides insight, via an audible signal perceptible to the human ear, into the fundamental electrochemical principles governing chemical transformations.

Abstract: Described herein is a method detecting a DNA methylation pattern in the small nuclear ribonucleoprotein polypeptide N (SNRPN) promoter region. Advantageously, the DNA is extracted from a dried blood sample from a human subject. In the method, treating the DNA with sodium bisulfite chemically modifies unmethylated cytosines in the DNA to uracil. The DNA is then amplified using specified forward and reverse primers for methylated and unmethylated SNRPN. The primer concentrations and amplification cycles are defined. A melting curve analysis of the PCR amplification products determines the DNA methylation pattern in the alleles in the DNA. The identified DNA methylation pattern can indicate normal status, Angelman syndrome or Prader-Willi syndrome.

Abstract: The present invention relates generally to methods and compositions useful for therapeutic vascular tissue engineering. In particular, the present invention provides methods for generating substantially pure populations of vasculogenic cells from human mesenchymal progenitors, and methods and compositions for clinical applications in the field of regenerative medicine.

Abstract: A Dectin-2 ligand vaccine adjuvant and a method of making and using the Dectin-2 ligand vaccine adjuvant in a vaccine to immunize a patient are disclosed. Also discloses is a vaccine composition comprising a Bl-Eng2 antigen and methods of using the vaccine composition to immunize a subject against a fungal infection.

Abstract: Methods for diagnosing propensity to exhibit acquired peripheral neuropathy in dogs are described. The methods and kits test dogs for presence of a disease-associated genomic variant. Presence of the genomic variant indicates an increased likelihood of the dog developing an acquired peripheral neuropathy. This information can be used to guide preemptive clinical treatment of the animal for peripheral neuropathy and to choose dogs for selective breeding programs.

Abstract: A method to make mono- or di-hydroxy-hexanoates. Triacetic acid lactone is converted to a ?-hexalactone or a 4-hydroxy-?-hexalactone intermediate. The intermediate is treated with an acid in the presence of an alcohol to yield a 5-hydroxy-hexanoate or 4,5-dihydroxy-hexanoate.

Abstract: In accordance with some embodiments, systems, methods, and media for generating digital images using low bit depth image sensor data are provided. In some embodiments, the system comprises: an image sensor; a processor programmed to: receive, from the image sensor, a series of low bit depth frames; provide low bit depth image information to a trained machine learning model comprising: a 3D convolutional layer; a 2D convolutional LSTM layer; a concatenation layer configured to generate a tensor that includes an output of the 2D convolutional LSTM layer and the low bit depth image information; and a 2D convolutional layer configured to generate an output based on the tensor; and generate a high bit depth image of a scene based on an output of the two-dimensional convolutional layer.

Abstract: Traveling-wave tube amplifiers for high-frequency signals, including terahertz signals, and methods for making a slow-wave structure for the traveling-wave tube amplifiers are provided. The slow-wave structures include helical conductors that are self-assembled via the release and relaxation of strained films from a sacrificial growth substrate.

Abstract: Described herein is a method of preparing an unbiased library of phage variants, comprising (a) preparing a population of “acceptor phage”; (b) removing an endogenous target gene and inserting gene variants into the acceptor phage genomes; (c) enriching the recombined phages; and (d) expressing the library for selection. The acceptor phage is a lytic phage comprising a synthetic genome wherein the target gene of interest is flanked by recombinase sites. The acceptor phage infects a first host bacteria expressing a recombination plasmid facilitating recombination. The phages then infect a second host bacteria expressing a counterselection system that accumulates recombined phage variants and selecting against non-recombined phages. The accumulated phage variants infect a third host bacteria. The phage library may then be sequenced and characterized.

Abstract: Methods of inducing mammalian cardiac cell proliferation by ectopically introducing an LRRC10 protein in a mammalian cardiac cell to thereby induce proliferation of the cardiac cell. The methods can be employed to induce heart regeneration, for example, after cardiac injury.

Abstract: A method to inhibit neutrophil recruitment to damaged tissue, thereby inhibiting inflammation in a subject. The method includes administering to an anti-inflammatory amount of a myeloid-derived growth factor (“MYDGF”). Also disclosed are corresponding pharmaceutical compositions of matter containing the MYDGF.

Abstract: Described herein are chemically defined, adherent culture protocols for generating functional motor neurons characteristic of diverse hindbrain and spinal cord regions, with high efficiency.

Abstract: Electrical stimulation of specific facial and lingual nerves creates a more sustained pulsatility activity compared to stimulation of other cranial nerves. Pulsatility of arteries has intrinsic time constraints related to the time for vasodilation/constriction and time to return to baseline (TBL) after electrical stimulation which may affect the pulsatility response. Control of temporal patterning and the stimulation waveform maximizes the physiological response to cerebral pulsatility and its resulting effects on cerebral spinal fluid penetration into the brain parenchyma for a multitude of therapeutic uses including clearing misfolded proteins and/or administered pharmacological agents, diluting endogenous neurochemical concentrations within the brain, and reducing non-synaptic coupling.

Abstract: A system and method for creating computed tomography (CT) images includes acquiring or accessing sinogram data and determining a photon count for the sinogram data. The method includes generating unbiased sinogram data from the sinogram data using an unbiased estimator and the photon count and reconstructing a CT image from the unbiased sinogram data.

Abstract: In accordance with some embodiments, systems, methods and media for single photon depth imaging with improved precision in ambient light conditions are provided. In some embodiments, the system comprises: a light source; a single photon detector; an attenuation element configured to provide a variable intensity attenuation factor; and a processor programmed to: (a)-determine an ambient light intensity associated with a scene point; (b)-select an attenuation factor based on the ambient light intensity; (c)-estimate a depth of the scene point based on a multiplicity of photon arrival times determined using the detector during a period of time during which light incident on the detector is attenuated by the selected attenuation factor and during which the light source is configured to periodically emit a pulse of light toward the scene point; (d)-repeat (a)-(c) for each of a multiplicity of scene points.

Abstract: Use of an AR antagonist, e.g., enzalutamide and other known AR antagonists, for reducing inflammatory genes of COVID-19 subjects is provided by the present invention. In other aspects, the present invention is directed to the use of an AR antagonist, e.g., the drug enzalutamide and other known AR antagonists, to treat lung cancer in a subject. In yet other aspects, the present invention is directed to an AR antagonist, e.g., the drug enzalutamide and other known AR antagonists, as an immunotherapy.

Abstract: Methods of transferring nanostructures from a first substrate to another substrate using a copolymer polymerized from one or more non-crosslinking monomers and one or more comonomers bearing crosslinkable groups as a transfer medium are provided. Relative to a poly(methyl methacrylate) homopolymer, the crosslinkable copolymers bond more strongly to the first substrate and, as a result, are able to transfer even very narrow nanostructures between substrates with high transfer yields.