Abstract: A system for monitoring and establishing physical properties of a subsurface formation at a testing site having a source well and one or more receiver wells may include a pump, a data collection system, and a controller. The controller may cause the pump to extract fluid from and inject fluid into the source well based on one or more measures indicative of an amount of liquid in the source well. The controller may receive measures related to pressures in the receiver wells and physical properties of the subsurface formation may be determined based on the measures related to pressures in the receiver well. In some cases, noise may be removed from the measures related to pressures in the receiver wells to facilitate determining the physical properties of the subsurface formation.

Abstract: The present invention provides methods for controllably forming a layer of amorphous ice and other amorphous solids on a substrate, and also provides cryo-electron microscopy (cryo-EM) sample preparation methods and systems that utilize in vacuo formation of amorphous ice and other solids. Formation of the amorphous solid layer can be independent of the deposition of sample molecules to be analyzed using electron microscopy, and allows for the generation of a uniformly thick layer. Optionally, mass spectrometry instruments are used to generate and purify molecules deposited on the generated amorphous solid layer. The techniques and systems described herein can deliver near ideal cryo-EM sample preparation to greatly increase resolution, sensitivity, scope, and throughput of cryo-EM protein imaging, and therefore greatly impact the field of structural biology.

Abstract: The present technology relates to compositions and methods for modulating expression of genes, which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and -B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.

Abstract: A platform and method for testing antibiotic sensitivity of a polymicrobial infection is provided. The platform includes a body defining a plurality of sets of chambers and a plurality of wells. Each set of chambers has a plurality of chambers adapted for culturing microbes of the polymicrobial infection therein. Each well is associated with a corresponding set of chambers and has an input in fluidic communication with the outlets of the plurality of chambers in the corresponding set of chambers. Selected antibiotic therapies may be received in the wells which fluidically connect the plurality of chambers in a corresponding set of chambers such that microbes cultured in the plurality of chambers in the corresponding set of chambers are in soluble factor contact.

Abstract: In accordance with some embodiments, systems, methods, and media for high dynamic range imaging using single-photon and conventional image sensor data are provided. In some embodiments, the system comprises: first detectors configured to detect a level of photons proportional to incident photon flux; second detectors configured to detect arrival of individual photons; a processor programmed to: receive, from the first detectors, first values indicative of photon flux from a scene with a first resolution; receive, from the second detectors, second values indicative of photon flux from the scene with a lower resolution; provide a first encoder of a trained machine learning model first flux values based on the first values, provide the second encoder of the model second flux values; receive, as output, values indicative of photon flux from the scene; and generate a high dynamic range image based on the third plurality of values.

Abstract: The present invention discloses an ionic liquid composition comprising an at least partially hydrophobic ionic liquid, wherein the at least partially hydrophobic ionic liquid comprises a dication comprising two monocationic groups linked by a bridging group wherein the bridging group provides an at least partially hydrophobic character. The composition may also include a hydrophilic ionic liquid. The hydrophobic ionic liquid may include a quaternary ammonium group which may be substituted or unsubstituted, saturated or unsaturated, linear, branched, cyclic or aromatic and the bridging group is a unsubstituted or substituted C3-C10 alkylene or C3-C10 alkoxyalkyl. Also disclosed is a nanoemulsion formulation which includes the ionic liquid compositions, at least one polymer, a hydrophobic liquid, an aqueous liquid, and a hydrophobic or hydrophilic therapeutic agent. Methods to deliver a therapeutic agent by delivering a nanoemulsion and methods to make a nanoemulsion are also disclosed.

Abstract: The present application relates to nucleic acids encoding polypeptides with ?-etherase activity; polypeptides with ?-etherase activity; vectors comprising said nucleic acids for the production of recombinant ?-etherase; cells, for example microbial cells transformed with nucleic acids encoding ?-etherase activity and vectors, including nucleic acids encoding ?-etherases; a composition comprising ?-etherases suitable for processing lignocellulose and a method that uses ?-etherases or compositions comprising ?-etherases in the processing of lignocellulose and related polysaccharides.

Abstract: A method for diagnosing or giving a prognosis for anxious temperament or trait-like anxiety in a human or non-human primate subject comprising the steps of (a) obtaining DNA from a blood or saliva sample from the subject and (b) quantifying methylation in a set of differentially methylated regions (DMRs) selected from SEQ ID NOs: 1-32, 34-44, 46-53, and 55-74, GCTCA, CGCACCG, AGAGGCAG, and AGCTCG or DMR-associated genes selected from DIP2C, GRB10, INPP5A, C17ORF97, PDXK, CACNA2D4, TRAPPC9, CRTC1, MEGF6, HIVEP3, OPCML, PITPNM2, ZFPM1, RAP1GAP2, NFATC1, RNF126, FSTL3, GNAS, SH3BP2, NEURL1B, MAD1L1, HSPA12B, IGF2, PEG10, PEG3, SLC16A3, SYTL1, and ZIM2, wherein a significant change methylation indicates the present of anxious temperament or trait-like anxiety, wherein the change is relative to DNA from a second human or non-human primate who does not have anxious temperament or trait-like anxiety.

Abstract: Differentially methylated positions associated with hypersomnolence, idiopathic hypersomnia, and long sleep time are provided herein along with biomarker panels thereof and methods of diagnosing and providing a prognosis of hypersomnolence, idiopathic hypersomnia, and long sleep time using said differentially methylated positions.

Abstract: A system and method are provided for magnetic resonance imaging (MRI) and/or image reconstruction that includes acquiring multi-pass, chemical shift-encoded (CSE)-MRI imaging data of a subject. The method further includes performing a complex, joint estimation of phase terms in the imaging data for each pass of the multi-pass, CSE-MRI imaging data to account for concomitant gradient (CG)-induced phase errors of different passes. The method also includes generating at least one of a proton density fat fraction (PDFF) estimate or an R2* estimate that is unbiased by CG-induced phase errors using the phase terms and communicating a report that includes at least one of the PDFF estimate or the R2* estimate.

Abstract: Improved methods of oxidative dehydrogenation (ODH) of alkanes and alkylbenzenes to the corresponding olefins are disclosed. The disclosed methods use ozone (O3) to mediate the oxidative dehydrogenation reaction with high selectivity for the desired product, and no heterogeneous ODH catalyst is needed.

Abstract: Disclosed are arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods using SNPs on genes of the bovine interferon tau signaling pathway for improved bovine fertilization rate, or embryo survival, or both.

Abstract: Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant ?-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

Abstract: Provided herein are peptides comprising an amino acid sequence having at least about 85% sequence identity to RYRPRAPIIAVT (SEQ ID NO: 1). These cationic peptides inhibit PKM2 methylation and may be used in the treatment of breast cancer and other diseases or conditions in which PKM2 is overexpressed. Such PKM2 peptides may be delivered to cancer cells using pH sensitive unimolecular nanoparticles comprising anionic polymers.

Abstract: In accordance with some aspects, systems, methods and media for hierarchical progressive point cloud rendering are provided. In some aspects, a method for point cloud rendering is provided, the method comprising: rendering a first image based on point cloud data; requesting point cloud points, first synthetic point cloud points, and an octant of a second synthetic point cloud that intersects a new viewing frustum; reprojecting points used during rendering of the first image into frame buffer objects (FBOs) of different resolutions; replacing reprojected points if a received point corresponding to the same pixel is closer to the camera; determining that a pixel in the highest resolution FBO is unfilled; copying a point that originated in a lower resolution FBO to the gap in the highest resolution FBO; and when the highest resolution FBO is filled, rendering a second image based on the contents.

Abstract: In some embodiments, systems, methods, and media for automatically identifying entrepreneurial individuals in a population using individual and population level data are provided. In some embodiments, a system is provided, comprising: a database storing: grades and identifying information for classes; a hardware processor configured to: calculate, for each class, a difficulty value based on the grade for each individual; modify grades associated with the individual based on the difficulties; determine a variance using the modified grades; determine an average variance; determine that the variance for a first individual is larger average; determine that the first individual is more likely than average to be entrepreneurial; in response to determining that the first individual is more likely than average to be entrepreneurial, add identifying information of the first student to a second database of potential entrepreneurs.

Abstract: A single treatment catheter is provided for delivery of brachytherapy radiation in combination with intratumoral injection into tumor tissue with a single skin and tumor insertion site. The catheter also enables precise delivery of anti-cancer agents, such as immunotherapy or other medical substances, to specific locations within the tumor with radiographic confirmation of the injection delivery area and location.

Abstract: Methods for generating human arterial endothelial cells under defined conditions in the absence of insulin are described. In particular, provided herein are efficient, defined, and scalable methods for generating human arterial endothelial cells from human pluripotent stem cells. Also provided herein are uses of human arterial endothelial cells obtained according to these methods. For example, methods of treating peripheral arterial disease and methods of screening agents for that effect adhesion of leukocytes to arterial endothelial cells are also provided.

Abstract: One or more example embodiments provide systems and methods for using impedance separation and impedance shaping.

Abstract: A controller for robot arms and the like having mechanical singularities identities paths near the singularities and modifies those paths to avoid excessive joint movement according to a minimization of tool orientation deviation to produce alternative paths that minimize changes in the tool orientation such as can affect application such as welding, sealant application, coating and the like.