Patents Assigned to Wisconsin Alumni Research Foundation
  • CELL-BASED DNA SENSORS AND METHODS OF USING SAME
    Publication number: 20240026466
    Abstract: Cell-based DNA sensors, compositions comprising the cell-based DNA sensors, and methods of detecting DNA and cells. The cell-based DNA sensors include competent cells that include genetic circuits. Each genetic circuit includes homology arms separated by an interstitial region that comprises at least one element of a reporter switch and/or a kill switch. The compositions include one or more cell-based DNA sensors. The cell-based DNA sensors can be used to detect DNA and cells by the genetic circuits undergoing homologous recombination with target regions of target DNA to activate or deactivate the reporter switch and/or kill switch.
    Type: Application
    Filed: December 16, 2022
    Publication date: January 25, 2024
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Ophelia Venturelli, Yu-Yu Cheng, Zhengyi Chen
  • Low-defect-density gamma phase aluminum oxide substrates for heteroepitaxial synthesis
    Patent number: 11879185
    Abstract: Aluminum oxide (Al2O3) thin films having a high ?-phase purity and low defect density and methods for making the aluminum oxide thin films are provided. Also provided are epitaxial heterostructures that incorporate the aluminum oxide thin films as growth substrates and methods of forming the heterostructures. The Al2O3 films are pure, or nearly pure, ?-Al2O3. As such, the films contain no, or only a very low concentration of, other Al2O3 polymorph phases. In particular, the Al2O3 films contain no, or only a very low concentration of, the ?-Al2O3 polymorph phase.
    Type: Grant
    Filed: December 7, 2021
    Date of Patent: January 23, 2024
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Chang-Beom Eom, Rui Liu, Paul Gregory Evans, Donald E. Savage, Thomas Francis Kuech
  • System and method for diffusion mapping using phasemodulated magnetic resonance imaging
    Patent number: 11874358
    Abstract: A system and method are provided for controlling a magnetic resonance imaging (MRI) system. The method includes performing a gradient echo pulse sequence that includes a phase increment of an RF pulse of the gradient echo pulse sequence selected to encode diffusion information into a phase of MR signals. The method also includes controlling the plurality of gradient coils and the RF system to acquire the MR signals as MR data, processing the MR data to determine MR signals corresponding to diffusion in the subject when acquiring the MR signals, and generating at least one of an image or a map of the subject indicating the diffusion in the subject from the MR data.
    Type: Grant
    Filed: March 15, 2022
    Date of Patent: January 16, 2024
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Daiki Tamada, Scott B. Reeder
  • Disposable speculum for digital otoscope
    Patent number: 11871908
    Abstract: A disposable speculum for an otoscope provides a sheath adapted to cover an elongate otoscope probe and providing inwardly extending tabs that are adapted to flex outward when the speculum is inserted over the otoscope to engage corresponding grooves of the otoscope. The inwardly extending tabs provide precise alignment of the speculum with the elongate otoscope probe along an insertion axis to protect a length of the probe.
    Type: Grant
    Filed: February 4, 2021
    Date of Patent: January 16, 2024
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James Berbee, Greg Rebella, Azita Hamedani, Patrick Ayerle, Thomas Hutcheson, IV, Ralph Paul
  • Alkali metal optical clock
    Patent number: 11868095
    Abstract: An atomic clock employs alkali metal atoms such as cesium normally used for microwave atomic clocks but with optical stimulation. While alkali metals provide light emissions having a spectral width being as much as 107 wider (and hence less precise) than alkali earth materials commonly targeted for optical atomic clocks, the present inventors have determined that this disadvantage is significantly reduced by improved signal-to-noise ratio in the obtained signal making practical an atomic clock with improved size, weight, and power consumption.
    Type: Grant
    Filed: March 15, 2022
    Date of Patent: January 9, 2024
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Mark Saffman, Shimon Kolkowitz, Arjav Sharma
  • Gene construct encoding mutant Thioesterase, mutant Thioesterase encoded thereby, transformed host cell containing the gene construct, and method of using them to produce medium-chain fatty acids
    Patent number: 11866744
    Abstract: Unnatural, mutated thioesterases having an amino acid sequence that is at least 80% identical to SEQ. ID. NO: 1 and having substitutions at one or more of amino acid positions I107, R108, L109, S122, M141, E142, Y145, and L146, gene constructs encoding and configured to express the mutated thioesterases in a transformed host cell and host cells transformed to contain the gene constructs.
    Type: Grant
    Filed: May 27, 2021
    Date of Patent: January 9, 2024
    Assignees: Wisconsin Alumni Research Foundation, The Penn State Research Foundation
    Inventors: Brian F. Pfleger, Nestor Jose Hernandez-Lozada, Costas Maranas, Matthew Grisewood
  • POLYPEPTIDES FOR DETECTION AND TREATMENT OF CORONAVIRUS INFECTION
    Publication number: 20240002477
    Abstract: To address the need in the art, the inventors have comprehensively characterized the SARS-CoV-2-specific B cell repertoire in convalescent COVID-19 patients and generated mAbs against the spike, ORF8, and NP proteins. Together, the inventors' data reveal key insight into antigen specificity and B cell subset distribution upon SARS-CoV-2 infection in the context of age, sex, and disease severity. Aspects of the disclosure relate to novel antibody and antigen binding fragments. Further aspects relate to polypeptides comprising the antigen binding fragment(s) of the disclosure, and compositions comprising the polypeptides, antibodies, and/or antigen binding fragments of the disclosure. Also described are nucleic acids encoding an antibody or antigen binding fragment of the disclosure.
    Type: Application
    Filed: December 3, 2021
    Publication date: January 4, 2024
    Applicants: The University of Chicago, Wisconsin Alumni Research Foundation
    Inventors: Patrick WILSON, Haley DUGAN, Christopher STAMPER, Yoshihiro KAWAOKA, Peter HALFMANN
  • Economical methods for performing oxidative catalytic pretreatment of plant biomass using a single-stage two oxidant process
    Patent number: 11859145
    Abstract: An improved alkaline pretreatment of biomass is provided that is a single-stage, two oxidant alkaline oxidative pretreatment process. The process uses a homogenous catalyst with at least two oxidants (Hydrogen peroxide and enhanced levels of oxygen) in an alkaline environment to catalytically pretreat lignocellulosic biomass in a single-stage oxidation reaction. The provided single-stage alkaline-oxidative pretreatment improves biomass pretreatment and increase enzymatic digestibility to improve the economic feasibility of production of lignocellulose derived sugars.
    Type: Grant
    Filed: August 29, 2022
    Date of Patent: January 2, 2024
    Assignees: Board of Trustees of Michigan State University, Montana State University, Wisconsin Alumni Research Foundation
    Inventors: Eric L. Hegg, Zhaoyang Yuan, David B. Hodge, Shannon S. Stahl, Bryan D. Bals
  • Multi-detector systems and methods for x-ray imaging
    Patent number: 11857355
    Abstract: A system and method for detection of x-rays is provided. An x-ray detector system may include an energy-integrating x-ray detector having an array of x-ray sensing elements that are configured to sense x-rays emitted from an x-ray source and generate energy-integrating x-ray data. The system may also include a photon-counting detector having another array of x-ray sensing elements configured to determine an interaction between individual x-ray photons with individual sensing elements of the another array of x-ray sensing elements to generate photon-counting x-ray data. The system may further include electronics configured to receive the energy-integrating x-ray data and the photon-counting x-ray data simultaneously.
    Type: Grant
    Filed: October 29, 2021
    Date of Patent: January 2, 2024
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventor: Ke Li
  • Biomarkers for early diagnosis and differentiation of mycobacterial infection
    Patent number: 11860168
    Abstract: Mycobacterial-specific biomarkers and methods of using such biomarkers for diagnosis of mycobacterial infection in a mammal are disclosed.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: January 2, 2024
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Adel Mohamed Talaat, Chia-wei Wu
  • Integration of semiconductor membranes with piezoelectric substrates
    Patent number: 11864465
    Abstract: Piezoelectrically actuated devices constructed from thin semiconductor membranes bonded directly to piezoelectric substrates are provided. Methods for fabricating these devices are also provided. The bonding of the semiconductor to the piezoelectric material does not require the use of any intermediate layers, such as bonding agents.
    Type: Grant
    Filed: May 22, 2020
    Date of Patent: January 2, 2024
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Max G. Lagally, Abhishek Bhat, Frank Steele Flack, Shelley Ann Scott, Robert H. Blick
  • Reconfigurable computer accelerator providing stream processor and dataflow processor
    Patent number: 11853244
    Abstract: A reconfigurable hardware accelerator for computers combines a high-speed dataflow processor, having programmable functional units rapidly reconfigured in a network of programmable switches, with a stream processor that may autonomously access memory in predefined access patterns after receiving simple stream instructions. The result is a compact, high-speed processor that may exploit parallelism associated with many application-specific programs susceptible to acceleration.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: December 26, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Karthikeyan Sankaralingam, Anthony Nowatzki, Vinay Gangadhar
  • Method and apparatus for improved security in trigger action platforms
    Patent number: 11856000
    Abstract: An apparatus and method for improving the security of trigger action platforms of a type providing interoperability between computer services send the trigger service additional information about an interoperability rule for the computer services so that the trigger service may implement a minimizer reducing the data communicated when the interoperability is implemented. Implementation of the minimizer may be done in a way that is transparent to the trigger action platform eliminating the need for disruption of existing interoperability services.
    Type: Grant
    Filed: June 4, 2021
    Date of Patent: December 26, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Yunang Chen, Mohannad Alhanahnah, Andrei Sabelfeld, Rahul Chatterjee, Earlence Fernandes
  • Humanized cell line
    Patent number: 11851648
    Abstract: A mammalian or avian cell line that expresses high levels of human influenza virus receptors is provided. In one embodiment, the cell line supports human influenza virus, e.g., human A/H3 influenza virus, isolation and growth much more effectively than corresponding conventional (unmodified) cells or in corresponding human virus receptor-overexpressing cells, and the propagated viruses may maintain higher genetic stability than in the corresponding cells.
    Type: Grant
    Filed: February 7, 2020
    Date of Patent: December 26, 2023
    Assignees: Wisconsin Alumni Research Foundation (WARF), The University of Tokyo
    Inventors: Yoshihiro Kawaoka, Kosuke Takada, Masaki Imai
  • Genetically modified genes and cells, and methods of using same for silencing virus gene expression
    Patent number: 11845958
    Abstract: Genetically modified CCNT1 and XPO1 genes encoding proteins that inhibit virus infection in cells. The genetically modified CCNT1 gene encodes a protein with a C261Y substitution with respect to the human CCNT1 protein. The genetically modified XPO1 gene encodes a protein with P411T, M412V, and/or F414S substitutions with respect to the human XPO1 protein. The genetically modified CCNT1 and XPO1 genes can be introduced in cells. The cells comprising the genetically modified CCNT1 and XPO1 genes can be introduced in a subject with a virus infection to treat the infection.
    Type: Grant
    Filed: September 5, 2019
    Date of Patent: December 19, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Nathan Mark Sherer, Ryan Thomas Behrens
  • Organelle-targeted genetically-encoded voltage indicators and methods of use thereof
    Patent number: 11846628
    Abstract: As described herein, a hybrid voltage sensor genetically-encoded voltage indicator (GEVI) for mitochondria or endoplasmic reticulum includes a transmembrane domain, and a fluorescent protein, wherein a terminus of the transmembrane domain and a terminus of the fluorescent protein are covalently linked directly or by a linker comprising 1 to 20 amino acids, and wherein the transmembrane domain comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or a peptide with greater than 85%, 90%, 95% or 98% identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. Also described are expression vectors, expression cassettes, and organelle membranes, as well as methods of determining the voltage across an organelle using the GEVIs.
    Type: Grant
    Filed: August 23, 2022
    Date of Patent: December 19, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Meyer Jackson, Masoud Sepehri Rad
  • 3D printed and in-situ poled flexible piezoelectric pressure sensor
    Patent number: 11849642
    Abstract: A piezoelectric artificial artery can be 3D printed to provide the real-time precise sensing of blood pressure and vessel motion patterns enabling early detection of partial occlusions. An electric-field assisted 3D printing method allows for rapid printing and simultaneously poled complex ferroelectric structures with high fidelity and good piezoelectric performance. The print material consists of ferroelectric potassium sodium niobate (KNN) particles embedded within a ferroelectric polyvinylidene fluoride (PVDF) polymer matrix.
    Type: Grant
    Filed: April 17, 2020
    Date of Patent: December 19, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Xudong Wang, Jun Li
  • COMPOUNDS AND METHODS FOR FORMING ION CHANNELS IN BIOLOGICAL MEMBRANES
    Publication number: 20230398091
    Abstract: Self-assembling compounds for the formation of ion channels in biological membranes include monoacylated benzo(crown-ether) (MAcBCE) compounds and monoalkylated benzo(crown-ether) (MAkBCE) compounds. Methods of preparing the MAcBCE and MAkBCE compounds and methods of forming an ion channel in a biological membrane are also disclosed.
    Type: Application
    Filed: August 4, 2022
    Publication date: December 14, 2023
    Applicants: Washington University, Wisconsin Alumni Research Foundation
    Inventors: Baron Chanda, Willy Carrasquel Ursulaez, Jennifer Schomaker, Corey Jones, Mahzad Dehghany
  • Isogenic blood-brain barrier model
    Patent number: 11840708
    Abstract: A method of creating an isogenic multicellular blood-brain barrier model from iPSCs is disclosed.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: December 12, 2023
    Assignees: WISCONSIN ALUMNI RESEARCH FOUNDATION, CEDARS-SINAI MEDICAL CENTER
    Inventors: Eric V. Shusta, Scott G. Canfield, Clive N. Svendsen, Sean P. Palecek, Gad D. Vatine
  • In vivo therapeutic delivery procedure and device
    Patent number: 11839761
    Abstract: A “localizable” systemic gene therapy system is provided substantially increasing the transfection efficiency of the gene vectors into targeted tissue cells and substantially reducing the escape of the gene vectors from the targeted tissue volume, such as would waste the vectors, promote undesired immune reactions, and/or incur prohibitive costs for the required dose of gene-containing virus vectors. In this regard, the invention provides a means to simultaneously achieve local electroporation and gene-containing vector injection in a portion of a vascularized organ. It includes two double-balloon catheters that create contained volumes in parallel blood vessels for the introduction of vectors with reduced loss along with electrodes providing electroporation of the cells in the same location where the vectors are injected.
    Type: Grant
    Filed: January 28, 2021
    Date of Patent: December 12, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Susan Hagness, Erik Aiken, John Booske, Hans Sollinger, Paul Laeseke, Tausif Alam