Abstract: The present invention relates to the field of biomaterials, more particularly to the field of poly(alkyl cyanoacrylate) based nano- and microfibers. The present invention provides a novel method for producing ready-to-use poly(alkyl cyanoacrylate) based nano/microfibers, wherein the method comprises electrospinning of poly(alkyl cyanoacrylate) homopolymers or copolymers generated by anionic polymerization of alkyl cyanoacrylate monomers or oligomers and characterized by a specific polydispersity index. Accordingly, the present invention also provides novel ready-to-use nano/microfibers obtainable by the method as well as uses thereof, including (therapeutic) biomedical applications such as wound healing, drug delivery and tissue regeneration and engineering.