Abstract: The present disclosure relates to non-surgical methods for delivery of an agent to the respiratory system of a subject, methods of treatment using delivery of agents to the respiratory system, devices for delivery of agents to the respiratory system, and use of such devices to deliver agents to the respiratory system. In certain embodiments, the present disclosure provides a provide a non-surgical method of delivering an agent to the respiratory system of a subject. The method comprises in vivo perturbation of the airway surface in one or more parts of the respiratory system in the subject and exposing the perturbed surface of the airway to the agent, thereby delivering the agent to the respiratory system of the subject.