Abstract: A method for producing a transgenic plant having increased content of 20-hydroxyecdysone according to an embodiment of the present invention may use insect-derived gene A method according to an embodiment of the present invention includes transforming a plant cell with a recombinant vector including at least one of a gene encoding short-chain dehydrogenase/reductase (SDR) protein and a gene encoding C-14 hydroxylase protein, a gene encoding C-25 hydroxylase protein, a gene encoding C-22 hydroxylase protein, a gene encoding C-2 hydroxylase protein, and a gene encoding C-20 hydroxylase protein derived from insect, and regenerating a plant from the transformed plant cell.

Abstract: A hematophagous arthropod repellent composition includes 3,4-dimethoxybenzaldehyde or vanillin acetate as an effective ingredient. The repellent composition is against hematophagous arthropods which transmit diseases to animals including humans or directly cause discomfort or stress by bloodsucking. The hematophagous arthropod repellent composition not only has excellent repellency against hematophagous arthropods such as mosquitoes and ticks but also has long-lasting repellency, and thus it may be used as a hematophagous arthropod repellent for protecting animals including humans.

Abstract: A method for producing a transgenic plant which has increased content of 20-hydroxyecdysone compared to a wild type plant includes transforming a plant cell with a recombinant vector containing a gene encoding CYP85 (cytochrome P450, 85 family) protein derived from spinach (Spinacia oleracea). A method for producing a transgenic plant with enhanced insect resistance includes transformation of a plant cell with a recombinant vector containing a gene encoding CYP85 derived from Spinacia oleracea.