Abstract: A method of preparing a recombinant adeno-associated virus (rAAV) including: (1) preparing a shuttle plasmid and a corresponding recombinant bacmid including a baculovirus genome, where the shuttle plasmid includes at least an rAAV gene of interest flanked by inverted terminal repeats (ITR-GOI) integrated with a heterologous functional gene fragment, and the recombinant bacmid includes an expression cassette of functional protein components necessary for assembly of the rAAV; (2) integrating the rAAV ITR-GOI and the expression cassette of functional protein components by using the shuttle plasmid and the recombinant bacmid, to yield a recombinant bacmid including a recombinant baculovirus genome; and (3) transfecting, with the recombinant bacmid, a host cell line.

Abstract: A method for producing a recombinant adeno-associated virus (rAAV) and a recombinant baculovirus virus, the method including: (1) infecting an insect host packaging cell line with a corresponding recombinant baculovirus integrated with an rAAV genome ITR-GOI (gene of interest flanked by AAV inverted terminal repeats) and an AAV Cap gene or AAV Rep gene; (2) culturing the host packaging cell line infected with the recombinant baculovirus, so as to produce the recombinant adeno-associated virus; and (3) separating and purifying the recombinant adeno-associated virus obtained in (2).

Abstract: An atomic sensing method, the method including providing a polarization converter; emitting a linearly polarized polychromatic laser beam to the polarization converter; converting, by the polarization converter, the linearly polarized polychromatic laser beam into a circularly-polarized laser beam and a linearly-polarized laser beam; combining the circularly-polarized laser beam and the linearly-polarized laser beam thereby yielding a multi-polarization polychromatic laser beam; transmitting the multi-polarization polychromatic laser beam to an atomic vapor cell comprising alkali metal atoms, polarizing the multi-polarization polychromatic laser beam into two laser beams, and detecting the two laser beams by two photodetectors, respectively.

Abstract: A method for producing recombinant adeno-associated virus, the method including: (1) transforming a gene of interest (GOI) into a recombinant baculovirus, where the recombinant baculovirus has a genome integrated with AAV Rep gene, Cap gene, and rAAV genome ITR-GOI that are needed in the production of the rAAV; and where the ITR-GOI is linked to expression cassette of the Cap gene and the Rep gene by a 5? terminal nucleic acid segment or a 3? terminal nucleic acid segment; (2) infecting host insect larvae with the recombinant baculovirus prepared in (1), such that the rAAV is produced in vivo in the host insect larvae; and (3) lysing the host insect larvae obtained in (2), and extracting and purifying the rAAV.

Abstract: An optical-fiber atomic light-filtering apparatus comprising an optical-fiber coupling focusing collimating mirror, a first polarizing optical fiber, a first permanent magnetic ring, a pure iron frame shaped like the Chinese character “”, a heat preservation box, a first capillary atomic cell, an armored twisted-pair heating wire, a second permanent magnetic ring, a second polarizing optical fiber, a thermostat, a cable, a third permanent magnetic ring, a temperature sensor, a second capillary atomic cell, a fourth permanent magnetic ring, a third polarizing optical fiber and a photoelectric detector. The two pairs of permanent magnetic rings are matched with the pure iron frame shaped like the Chinese character “” to provide magnetic fields for the two capillary atomic cells working in the same temperature environment; a polarizing plane changes after interaction between a weak signal light and atoms.

Abstract: An inert gas nucleus channel device comprising a magnetic resonance control system, a radio frequency generator, an RF amplifier and AD converter, a frequency synthesizer, a first mixer, a second power amplifier, a second T/R switch, an inert gas nucleus coil, a second pre-amplifier and a second mixer. A magnetic resonance imaging method is also disclosed. The method controls an increase or decrease in a frequency of a pulse or echo signal excited by a radio-frequency signal, implementing magnetic resonance imaging using hyperpolarized inert gases (such as xenon, helium, and krypton), making it possible to apply MRI to lungs and brains using the hyperpolarized inert gases, and expanding the probing range of MRI. The inert gas nucleus channel device is simple in structure and easy to operate and upgrades a hydrogen nucleus magnetic resonance imaging instrument to become a multi-nuclei MRI system using the hyperpolarized inert gases.

Abstract: A recombinant baculovirus, including: an adeno-associated virus Rep gene, an adeno-associated virus Cap gene, and an recombinant adeno-associated virus genome ITR-GOI (gene of interest) flanked by rAAV inverted terminal repeats (ITR). The ITR-GOI includes a 5? terminal nucleic acid fragment and a 3? terminal nucleic acid fragment. The ITR-GOI is linked to an expression cassette of the Cap gene and an expression cassette of the Rep gene through the 5? terminal nucleic acid fragment and the 3? terminal nucleic acid fragment, respectively.

Abstract: An electron-nuclear double resonance resonator, having a loop-gap resonator and an elongated lead; the loop-gap resonator comprises a plurality of arc-shaped conductive plates, and the elongated lead connects the arc-shaped conductive plates into a radio-frequency coil; the loop-gap resonator resonates at an electron resonance frequency, and the radio-frequency coil resonates at a nuclear resonance frequency; with the structure of the loop-gap resonator, the separation between an electric field and a magnetic field can be accelerated to ensure the maximization of the ratio of the magnetic field to the electric field inside a resonant resonator; and with the elongated lead, the impact of the lead to a resonance frequency and the mode of the loop-gap resonator is prevented as much as possible, and meanwhile the conductive plates of the loop-gap resonator can be connected into the radio-frequency coil.

Abstract: A physical unit of a chip-scale nuclear magnetic resonance (NMR) gyroscope, the physical unit including: a vertical cavity surface emitting laser (VCSEL), a silicon sheet including a recess, a glass sheet, an atomic vapor chamber, a first right angle prism, a quarter-wave plate, a polarizing beam splitter, and photodetectors. The recess includes sides including reflecting mirrors. The glass sheet is disposed on the silicon sheet. The recess of the silicon sheet is in a structure of an inverted square frustum, and the reflecting mirrors are disposed on sides of the recess. The atomic vapor chamber is an enclosed region formed between the recess and the glass sheet. The atomic vapor chamber is filled with alkali metal atoms, one or a plurality of inert gas atoms, and one or a plurality of buffer gases.

Abstract: A physical system for a chip-scale coherent population trapping (CPT) atomic clock. The physical system includes: a vertical-cavity surface-emitting laser (VCSEL) device, a first polarizing beam splitter, a first ?/4 wave plate, a chip of an atomic vapor cell, a second ?/4 wave plate, a reflection device, a lens, a second polarizing beam splitter, and a photo detector. The first polarizing beam splitter, the first ?/4 wave plate, the chip of the atomic vapor cell, the second ?/4 wave plate, and the reflection device are disposed in sequence. The lens, the second polarizing beam splitter, and the photo detector are disposed in sequence.

Abstract: A device for producing a coherent bi-color light source, including: an array substrate, a first laser tube driven by a first direct current signal, a second laser tube driven by a modulation signal coupled by a microwave signal and a second DC signal, a half wave plate, a birefringent crystal, a first quarter wave plate, a partially reflecting plane mirror, and a second quarter wave plate. The first laser tube and the second laser tube are fixed on the array substrate. The half wave plate, the birefringent crystal, the first quarter wave plate, the partially reflecting plane mirror, and the second quarter wave plate are disposed in sequence in an emission direction of a laser beam emitted by the first laser tube. The second laser tube is disposed opposite to the birefringent crystal.

Abstract: A method for forming an atomic clock, including: a) connecting an output terminal of a current source to a DC input terminal of a DC bias element, and connecting an output terminal of a microwave source to a high-frequency RF input terminal of the DC bias element through a microwave switch to generate a circular polarization laser; b) feeding the circular polarization laser into an atom sample bubble to interact with an alkali-metal atom, and controlling the current source through control equipment; c) modulating output current, and demodulating detection light intensity; d) controlling the microwave switch to produce a Ramsey-CPT interference fringe; and e) modulating the microwave frequency, demodulating light intensity, employing a central fringe as a frequency discrimination signal, and locking the microwave frequency at maximum peak position of the central fringe to output stable frequency of the atomic clock.

Abstract: The present invention discloses a free-space quantum communication device with atomic filters which are used in the field of quantum cryptography. The free-space quantum communication device of the present invention includes a beam splitter (2), two ?/2 wave plates (3,10), a ?/4 wave plate (4), two polarization beam splitters (5,11), two atomic filters (6,12), two light couplers (7,13), two optical fibers (8,14) and two single-photon detectors (9,15). In the present invention, the interference filter used in the conventional quantum communication device is replaced by ultra-narrow line-width atomic filters. As the pass-band width of atomic filter is narrower than that of the interference filter in magnitude order of 3 and a light noise rejection ratio of 10?5 can be obtained, error rate generated by background light noise can be reduced by magnitude order of 2-3. Further, the distance for carrying out free-space quantum communication can be increased.

Abstract: The present invention relates to a kind of microwave cavity for atomic frequency standard, which consists of slotted tube, cylindrical cavity body, dielectric ring, heating filament, screw bolt for adjustment of cavity frequency. Its characteristics are listed as follows: 1. There are slots and electrodes at the upside of slotted tube. 2. The slotted tube and the bottom cap of the cavity are made as a whole. 3. The Rb absorption cell is inserted in the slotted tube. 4. The dielectric ring is inserted between the slotted tube and the cylindrical cavity body. 5. There is one or more screw hole in the wall of cylindrical cavity body. 6. One or more screw bolt for adjustment of cavity frequency is installed into the screw hole.