Abstract: The present disclosure describes a field flow fractionator including (a) a top plate assembly including a top electrically conductive electrode, (b) an o-ring, (c) an electrically insulating frit, (d) an electrically insulating spacer between a bottom surface of the top electrode and the o-ring and the frit, (e) a membrane between the spacer and the frit, and (f) a bottom plate assembly including a bottom electrically conductive electrode.

Abstract: The present disclosure describes a computer implemented method, a system, and a computer program product of determining intrinsic viscosity and Huggins constant of an unknown sample. In an embodiment, the method, system, and computer program product include, receiving specific viscosity values over time from a viscometer corresponding to a series of aliquots of an unknown sample injected into an instrument chain where the instrument chain includes the viscometer, calculating a total mass of each of the aliquots, calculating a first intermediate viscosity value of each of the aliquots, calculating a second intermediate viscosity value of each of the aliquots, and fitting the total mass, the first intermediate viscosity value, and the second intermediate viscosity value to a fitting, resulting in a calculated intrinsic viscosity of the unknown sample and a calculated Huggins constant of the unknown sample.

Abstract: The present disclosure describes a differential refractometer for gradient chromatography. In an exemplary embodiment, the differential refractometer includes a solvent delay volume, an eluent flow meter coupled to an eluent inlet of a sample cell, a solvent flow regulator coupled to an outlet of the solvent delay volume and coupled to a solvent inlet of a reference cell, an instrument controller configured to receive the eluent flow rate from the eluent flow meter, configured to receive the solvent flow rate from the solvent flow regulator, configured to receive a flow rate ratio from a flow rate ratio data source, wherein the flow rate ratio indicates a ratio of the eluent flow rate to the solvent flow rate, and an optical bench configured to measure a difference between a refractive index of the eluent present in the sample cell and a refractive index of the solvent present in the reference cell.

Abstract: The present disclosure describes an apparatus, method, system, and computer program product of identifying a cuvette for measuring light scattering of a sample. In an exemplary embodiment, the apparatus includes at least one sensor configured to detect at least one shape feature of a cuvette, and an insulating block configured to maintain a temperature of the at least one sensor within an operating temperature range of the at least one sensor. In an exemplary embodiment, the method, system, and computer program product include receiving a first set of signals from a first sensor directed to a cuvette, receiving a second set of signals from a second sensor directed to the cuvette, and executing a set of logical operations detecting a shape feature of the cuvette.

Abstract: The present disclosure describes a computer implemented method, a system, and a computer program product of measuring quality attributes of a virus sample. In an embodiment, the method, system, and computer program product include (1) receiving light scattering (LS) data from a light scattering detector analyzing separations of a virus sample, dRI data, and UV data, (2) receiving a molecular weight of a protein component of the sample, Mprotein (expected), a refractive index increment of the protein component, (dn/dc)protein, a refractive index increment of a DNA component of the sample, (dn/dc)DNA, and an extinction coefficient of the protein component, ?protein, (3) calculating a protein fraction of the sample, xprotein, with respect to the LS data, the dRI data, the Mprotein (expected), the (dn/dc)protein, the (dn/dc)DNA, and an optical constant, K, (4) calculating a DNA extinction coefficient of the sample, ?DNA, and (5) calculating quality attribute values of the sample.

Abstract: Described is a field flow fractionator including (1) a top plate assembly including a first non-corrosive material, at least three fluid fittings machined into the first non corrosive material, a top cavity machined into the first non-corrosive material, and at least one top plate o-ring configured to form a horizontal geometry of a separation channel, (2) a membrane, (3) a bottom plate assembly including a second non-corrosive material, a bottom cavity machined into the second non-corrosive material, a frit configured to be placed into the bottom cavity, and at least one bottom plate o-ring configured to seal the bottom plate assembly to the top plate assembly, such that a top surface of the second non-corrosive material and a top surface of the frit are machined to be coplanar, and (4) where the top plate assembly, the membrane, and the bottom assembly define the separation channel.

Abstract: The present disclosure describes an apparatus, method, and system of regulating a detector flow of a field flow fractionator. In an embodiment, the apparatus includes (1) a detector flow meter, where the detector flow meter is configured to measure a detector flow from the field flow fractionator, (2) a channel pressure meter, where the channel pressure meter is configured to measure a channel pressure of the field flow fractionator, (3) at least one control valve, where an inlet of the at least one control valve is connected to an outlet of the channel pressure meter, (4) where the detector flow meter is configured to set a channel pressure set point of the channel pressure meter, and (5) where the channel pressure meter is configured to actuate the at least one control valve to maintain a channel pressure of the field flow fractionator at the channel pressure set point.

Abstract: The present disclosure describes a computer implemented method, a system, and a computer program product of controlling the purification of a macromolecule solution via real-time multi-angle light scattering.

Abstract: Embodiments provided herein relate to methods and compositions for immunological analysis. Some embodiments relate to methods and compositions for characterizing a sample by identifying the TCR alpha chain or beta chain of a T cell.

Abstract: The present disclosure describes a computer implemented method, a system, and a computer program product of calculating molar mass values of components of and molar concentration values of conjugate molecules/particles.

Abstract: The present disclosure describes a flow cell, a read head, and a skid attachment for measuring real-time molecular weight for downstream process control. In an embodiment, the flow cell comprises a hollow cylindrical tube, an inlet flange connected to an inlet of the tube, and an outlet flange connected to an outlet of the tube. In an embodiment, the read head comprises at least one push rod, at least two line contacts, where the at least one push rod is configured to push an outer side wall of a flow cell against the at least two line contacts. In an embodiment, the skid attachment comprises a plurality of arms connected to an enclosure configured to house at least a multi-angle light scattering instrument comprising a read head.

Abstract: The present disclosure describes a computer implemented method, a system, and a computer program product of purifying a sample solution via real-time multi-angle light scattering. In an embodiment, the method, system, and computer program product include receiving from a MALS instrument baseline scattering intensity values of a pure buffer, receiving from the MALS instrument scattering intensity values of a sample solution, and characterizing at least one component of the sample solution, resulting in a time series of values of a dimension, D, of the at least one component and a time series of values of excess Rayleigh ratio, R0, of the at least one component, and determining that the values of the dimension, D, fall within a dimension, D, value range and that the values of excess Rayleigh ratio, R0, fall within an excess Rayleigh ratio value range, and transmitting a collect sample solution command to collect the sample solution.

Abstract: The present disclosure describes a field flow fractionator (FFF). In an embodiment, the FFF includes a spacer including a core, and a coating covering the core. In an embodiment, the FFF includes a spacer including a core, and a coating covering the core, a bottom block including pins, and a top block including mating holes to receive the pins, where the spacer is configured to be positioned between the top block and the bottom block. In an embodiment, the FFF includes a spacer including a core including an opening, a top side, and a bottom side, a first coating covering the top side, and a second coating covering the bottom side, a top block configured to press on the first coating along a first periphery of the opening, and a bottom block configured to press on the second coating along a second periphery of the opening.

Abstract: The present disclosure describes a method, a system, and a computer program product of indicating a status of an analytical instrument on a screen of the analytical instrument. In an embodiment, the method, the system, and the computer program product include receiving data from an analytical instrument monitoring a liquid sample, segmenting the received data into data segments for at least two characteristics of at least one of the instrument, the sample, and an operating environment of the instrument, analyzing each of the data segments for the at least two characteristics, retrieving threshold values for the at least two characteristics from a computer data source, calculating at least one status of at least one of the instrument, the sample, and the operating environment, with respect to the analyzed data segments and the threshold values, and displaying the at least one status on a display of the instrument.

Abstract: An apparatus for the high throughput measurement of optical properties of liquid samples placed into the wells of a multiwell plate is disclosed. An optical fiber within a fiber bundle containing no corrective optics between the fiber ends and the well plate bottom illuminates the sample in order to induce fluorescence, and multiple fibers collect emission radiation and transmit it to a fluorescence detector such as a spectrometer. Other embodiments involve a light scattering illumination source with detection fibers located in either the same bundle containing the fluorescence monitoring fibers or an independent light scattering detection bundle for the measurement of static and/or dynamic light scattering. Some embodiments of the invention permit the measurement of phase analysis light scattering. Thus the measurement of multiple optical properties of a liquid sample may be made simultaneously or in succession. A method for these measurements is also disclosed.

Abstract: The present disclosure describes a field flow fractionator including (1) a top plate assembly including (a) a first non-corrosive material, and (b) at least three fluid fittings machined (simpler) into the material, (2) a spacer, (3) a membrane, (4) a bottom plate assembly including (a) a second non-corrosive material, (b) a cavity machined into the second non-corrosive material, (c) a frit configured to be placed into the cavity, and (d) at least one bottom plate o-ring configured to seal the bottom plate assembly to the spacer, and (5) where the top plate assembly, the spacer, the membrane, and the bottom assembly define a separation channel. In an embodiment, the at least three fluid fittings including a fitting for an in-flow, a fitting for an out-flow, and a fitting for a cross-flow.

Abstract: The present disclosure describes an apparatus of measuring light scattering of a sample. In an embodiment, the apparatus includes (1) at least one display logically coupled to an enclosure housing a light scattering measurement instrument, where the at least one display is configured to allow for operating the instrument to acquire light scattering data from the sample and for accessing the data, (2) an indicator connected to an outside surface of the enclosure, where the indicator is configured to indicate at least one status of the instrument, (3) a sample chamber configured to accommodate at least one sample cell, where each of the at least one sample cell has a unique size and a unique shape, and (4) and a sample door connected to the enclosure, where the sample door is configured to seal the sample chamber, thereby providing thermal insulation to the sample chamber.

Abstract: The present disclosure describes an apparatus, method, system, and computer program product of detecting bubbles in images of a sample in wells of a well plate. In an exemplary embodiment, In an exemplary embodiment, the computer implemented method, the system, and the computer program product include receiving, by a computer system, at least one image of at least one well of a well plate containing a liquid sample, executing, by the computer system, a set of logical operations cropping the image to include a well of interest, and executing, by the computer system, a set of logical operations processing, by a trained convolutional neural network, the cropped image, resulting in a probability that the image depicts at least one bubble.