Patents Assigned to Wyeth Research Ireland Limited
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Patent number: 7906024Abstract: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration.Type: GrantFiled: January 24, 2006Date of Patent: March 15, 2011Assignee: Wyeth Research Ireland LimitedInventors: James Pierce, Enda Moran
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Patent number: 7490508Abstract: An apparatus for testing a cleaning procedure for a material. The apparatus includes a rack having a seat configured to retain a plurality of test coupons at a predetermined angle, an upper tray that distributes a solution along the lines of the rack, a lower tray for receiving solution passed over coupons disposed on the rack, a meter that gauges a flow rate of the solution, a thermostatic heater adapted to bring the solution to a predetermined temperature, and a variable speed pump that directs the solution from a reservoir to the upper tray.Type: GrantFiled: October 12, 2005Date of Patent: February 17, 2009Assignee: Wyeth Research Ireland LimitedInventors: Alfredo J. Canhoto, Rod J. Azadan, John Putnam, Michael Kreuze, Brian Williams, Kristen Nobles, Jeff Chapman, Kelli Barrett
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Patent number: 7335491Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: February 26, 2008Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Patent number: 7300773Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: November 27, 2007Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Patent number: 7294484Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: November 13, 2007Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Publication number: 20060194313Abstract: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration.Type: ApplicationFiled: January 24, 2006Publication date: August 31, 2006Applicant: Wyeth Research Ireland LimitedInventors: James Pierce, Enda Moran
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Publication number: 20060160180Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: ApplicationFiled: August 25, 2005Publication date: July 20, 2006Applicant: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James Mercer, Wenge Wang, Daniel Lasko
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Publication number: 20060121569Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: ApplicationFiled: August 25, 2005Publication date: June 8, 2006Applicant: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James Mercer, Wenge Wang, Daniel Lasko
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Publication number: 20060121568Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: ApplicationFiled: August 25, 2005Publication date: June 8, 2006Applicant: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James Mercer, Wenge Wang, Daniel Lasko