Abstract: The invention is directed to a sandwich hybridization assay wherein a nucleic acid capture probe is firstly immobilized on an assay plate via masked receptors on the plate. The capture probe is immobilized by the binding of receptor ligands on the capture probe during this first step. Subsequently, target nucleic acid is hybridized to the immobilized capture probe either before or after the hybridization of an indicator nucleic acid probe onto the target. The target is quantified via detection of the immobilized indicator signal.