Abstract: The present application discloses a primer set, a reagent, a kit for detecting methylation of a specific region of a human colorectal cancer-related gene from feces, blood, or tissue by using specific PCR, and the application thereof. The primer set includes specific amplified primer probe sequences for the methylated regions of specific regions of the gene markers PRDM12, FOXE1, and B3GAT2 genes found in this study; and the primer probe group also includes the specific amplification primer probe sequence of the methylated region of the specific region of the Vimentin, SFRP2-1, and SFRP2-2 genes and the specific amplification primer probe group of the internal reference gene ACTB, thus having high detection efficiency and strong pertinence.