Abstract: An improved apparatus and method for evaluation of focal length and transparency of vertebrate eye lenses is provided. Preferably with the aid of an alignment camera, a lens horizontally mounted in a transparent container is positioned with the axis of the lens aligned with scanning direction of a laser projected vertically through the lens. The laser is scanned across the lens, and an analysis camera at ninety degrees to the scanning path captures images of the path of the laser beam through the lens. The images are then analysed for determination of focal length, spherical aberration, and the like. Changes over time in response to a stimulus may be evaluated.