Abstract: The present invention relates to an inert carrier Salmonella and potential use thereof, which is expected to be developed into a new inert carrier bacteria, and can be applied to the development of an indirect agglutination test method for simple and rapid detection of antigens or infected antibodies. The inert carrier Salmonella has been deposited in CGMCC in Beijing on Mar. 18, 2019 with the accession number of CGMCC No.17340, and is classified as Salmonella sp.with a strain code of S9. The Salmonella has no visible agglutination reaction with various chicken sera derived from different genetic backgrounds, i.e., it has no non-specific agglutination reaction with chicken sera derived from broad range of genetic backgrounds.

Abstract: Disclosed are a method and a system for bug localization based on a code knowledge graph, including the steps of: extracting source codes from a Git version control system, parsing in the source codes to generate an abstract syntax tree (AST), constructing a code knowledge graph, preprocessing the summary and description of a bug report crawled from a Bugzilla bug tracking system, and performing the named entity recognition to identify bug-related entity sequence, converting the code knowledge graph and the bug-related entity sequence into vector representation through an embedding algorithm, calculating cosine similarities of vector representations between the code knowledge graph and the bug entity sequence, ranking the similarities from high to low to generate a list of suspicious methods, filtering redundant information in the source codes, identifying bug-related entity elements in the bug report, and reserving the bug-related information.

Abstract: An indirect selective breeding method of high-collagen meat geese includes : (1) obtaining a curve equation y=0.017x+0.026; (2) rearing goslings to young geese; (3) measuring the length, width, and height values of the knobs of the geese, and calculating to give the collagen content according to the curve equation obtained in step (1); (4) sorting the geese in descending order according to the collagen content and keeping the geese with collagen content ranked top 50% as breeding geese; (5) after the geese lay eggs, hatching the goose eggs into goslings; and (6) repeating steps (2)-(5) for selective breeding over generations. The indirect selective breeding method is simple and easy to implement. A new strain of meat geese with high collagen content can be obtained from selective breeding simply by measuring the length, width, and height of the knobs of geese of each generation.

Abstract: Disclosed is an intelligent illumination seedling incubator for sorghum culture. The incubator comprises an incubator body and a culture component. At least one installation opening is formed in the forward side of the incubator body. The culture component comprises culture trays and a vertically arranged connecting shaft which is connected to the incubator body. A plurality of non-circular support pillars are arranged on the connecting shaft at intervals in the height direction. The culture trays are installed in the incubator body through the installation opening. A clamping opening is formed in the culture tray. A connecting sink with the same shape as the support pillar is formed in the downward end of the culture tray inside the clamping opening. After the culture tray is clamped into the connecting shaft through the clamping opening, the culture tray is exactly inserted on the support pillar through the connecting sink.

Abstract: The present disclosure relates to a PCR detection kit for rapidly identifying Salmonella of specific serotypes. The kit includes primers for detecting tcpS gene, the primers for detecting the tcpS gene including a forward primer having a nucleotide sequence as set forth in SEQ ID NO. 1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO. 2. The kit can identify Salmonella of enteritidis, pullorum/gallinarum, and dublin serotypes rapidly and in a high throughput, which can be used as an auxiliary method for the conventional serotyping of Salmonella.

Abstract: A generic inert bio-vector Salmonella sp. S9H and potential uses thereof are provided. The generic inert bio-vector Salmonella sp. S9H is derived from a continuous in-vitro culture of an inert bio-vector bacterium Salmonella sp. S9 by using LB solid and liquid culture media for passage to the fortieth generation. With a quantity of bacteria at a working concentration, the S9H does not cause non-specific agglutination reactions in sera or whole blood derived from humans, mice, cattle, pigs and poultry (including chickens, ducks, geese, turkeys, pigeons and quails); moreover, S9H has a property of carrying, and expressing and displaying different antigen factors derived from humans, mice, cattle, pigs and poultry (including chickens, ducks, geese, turkeys, pigeons and quails) on the surface thereof .

Abstract: A method for optimizing the blade axis position of a water pump under all operating conditions which includes determination of multiple calculation conditions within the range of all the operating conditions of the water pump, three-dimensional modeling and mesh generation of the calculation area of the flow field of the water pump at multiple blade angles, numerical simulation of the flow field and calculation and determination of blade hydraulic torques under multiple conditions, determination of the range of the position of the blade resultant hydraulic pressure action line and an optimal blade axis position under all the operating conditions, determination of the small region of the optimal blade axis position under all the operating conditions, determination of the optimal blade axis position, and comparison of the blade hydraulic torques before and after optimization of the blade axis position.

Abstract: Provided is a recombinant H7N9 subtype avian influenza virus, a marked vaccine and a preparation method thereof. For the recombinant H7N9 subtype avian influenza virus, a strain JD/17 of H7N9 subtype avian influenza virus is used as parent virus and a peptide sequence in HA protein of the strain JD/17 is replaced with a peptide sequence in HA protein of H3 subtype; the strain JD/17 of H7N9 subtype avian influenza virus has a preservation number of CCTCC No. V201862. The results of HA titers, EID50, TCID50 show that the rescued virus maintains similar biological characteristics of parent virus, such as high HA titers and EID50, and chickens immunized with the marked inactivated and emulsified vaccine produce a high level of antibody, and this antibody can be distinguished from antibodies produced by chickens naturally infected with H7N9 subtype avian influenza virus.

Abstract: The present invention discloses a method for confined impinging jets (CIJ) mixing with imbalanced momenta. The method includes the following steps: connecting each inlet of a CIJ mixer with a to-be-mixed fluid by using an inlet conduit; connecting an outlet of the mixer with an inlet of a suction device by using an outlet conduit; and starting the suction device, enabling the to-be-mixed fluids to enter the mixer sequentially through the conduits and the inlets of the mixer and to mix in a mixer chamber, and the mixture is then sucked out from the outlet of the mixer and flows sequentially through the conduit, the inlet of the suction device, and the outlet of the suction device.

Abstract: A prestress-free self-centering energy-dissipative tension-only brace, including a self-centering mechanism, an energy dissipation mechanism, a force-limiting energy-dissipative mechanism, a high strength steel cable, and a sleeve. The self-centering mechanism includes a sliding end plate, a spring, and a connection rod. One end of the spring is connected and fixed to the inner wall of the sleeve, and the other end of the spring is connected with the sliding end plate. One end of the connection rod is anchored at the center of the sliding end plate, and the other end of the connection rod passes through a fixed end of the spring. The energy dissipation mechanism includes rotating shafts, rotating wheels, friction plates, chains, and a cross-shaped connecting piece.

Abstract: The present disclosure relates to a PCR detection kit for rapidly identifying Salmonella of specific serotypes. The kit includes primers for detecting tcpS gene, the primers for detecting the tcpS gene including a forward primer having a nucleotide sequence as set forth in SEQ ID NO. 1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO. 2. The kit can identify Salmonella of enteritidis, pullorum/gallinarum, and dublin serotypes rapidly and in a high throughput, which can be used as an auxiliary method for the conventional serotyping of Salmonella.