Abstract: The present invention is directed to a method for cloning and producing the Spiroplasma sp. strain MQ1 DNA methylase by (1) introducing the Spiroplasma methylase gene into a host whereby the methylase gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Spiroplasma methylase and (3) purifying the Spiroplasma methylase from the fermented host which contains the vector encoding and expressing the Spiroplasma DNA methylase activity.
Type:
Grant
Filed:
October 4, 1991
Date of Patent:
March 22, 1994
Assignee:
Yissum Research Development Company of the Hebrew University of Jerusalem, an Israeli Corp.
Inventors:
Aharon Razin, Shlomo Rottem, Pinhas F. Renbaum