Abstract: Disclosed are persimmon vinegar and a preparation method therefor. The persimmon vinegar, which is good for health, is prepared from astringent persimmon fruits, which are disadvantageous in taste, by alcohol fermentation and acetic acid fermentation, in sequence. For the alcohol fermentation, the novel strain, Saccharomyces kluyveri DJ97 (KCTC 0591BP) is used. In combination with various additives, such as polydextrose, liquid fructose, concentrated pear extract, honey, citric acid, sodium citrate, etc., the persimmon vinegar can provide a beverage.