Abstract: A stabilized human monoclonal antibody preparation containing 1 to 20 mg of D-mannitol per 1 mg of a human monoclonal antibody. This preparation is excellent in stability in a solution state, a freeze drying state and a freezing state, particularly stability against aggregation and precipitation of the human monoclonal antibody at the time of redissolution after freeze drying.

Abstract: A vector plasmid containing(a) the OriA region of plasmid pBA1 derived from Anacystis nidulans,(b) the multicloning region and(c) the region of colicin E1 plasmid, which contains the OriE region thereof and wherein the gene defined by the rop region thereof is removed.This plasmid is replicable in the cells of both Escherichia coli and cyanobacteria and thus useful as a shuttle vector.

Abstract: A method for expressing a physiologically active polypeptide in cells of a blue-green alga by transforming the cells of a blue-green alga with a recombinant DNA containing a structural gene encoding the polypeptide, characterized in that as the recombinant DNA is used a recombinant DNA which contains a structural gene encoding the physiologically active polypeptide, the transcription initiation region of the RuBisCO gene of Anacystis nidulans located upstream of the structural gene, and the transcription termination region of the RuBisCO gene located downstream of the structural gene. According to this method, physiologically active polypeptide can efficiently be expressed using blue-green algae.

Abstract: Two penta-peptides, Asp-Asp-Asn-Asp-Asn and Asp-Asp-Ser-Gln-Gln, extracted from green barley leaves, have been found to inhibit the adenosine diphosphate (ADP)-induced aggregation of human blood platelets.

Abstract: A new cell line TriH8 is obtained by fusing a particular sub-clone, A431c, of the human epidermoid carcinoma cell line A431 with the TOS/H8 (human/human) hybridoma. Fused cell TriH8 has been deposited under FRI accession number FERM BP-4452. The fused cell is capable of proliferating in basal medium without addition of serum or growth factor. The cell line is more effective in producing antibody (IgM) than the donor cell TOS/H8.

Abstract: Amino acid sequences of the H chain and L chain variable regions of mouse monoclonal antibodies Idio 3, Idio 17, Idio 20, Idio 27 and Idio 33 against idiotypes of a cancer cell antigen-specific human immunoglobulin CLN/IgG produced by a human/human fused cell strain CLN/SUZ H11, and base sequences of the genes of the variable regions are disclosed. The above amino acid sequences and the base sequences are useful in medical and pharmaceutical fields such as prophylaxis, treatment and/or diagnosis of human diseases, and/or in pharmacological and/or biochemical fields, etc. such as biochemical reagents, and reagents for purification of biomacromolecules.

Abstract: An agent for preserving the freshness of plants containing 2"-O-glucosylisovitexin as an effective ingredient is disclosed. This agent is excellent in an effect of preserving the freshness of cut flowers and potted plants, and, moreover, is safe and has no problem of the pollution of the environment.

Abstract: A pressed juice of green leaves of true grasses or its dry powder wherein the total amount of chlorine (Cl) and nitrate group (NO.sub.3) is not more than 9% by weight based on the solids content, and a process for producing same using an ion exchange membrane or an ion exchange resin. The above pressed juice or its dry powder is substantially free from additives as a natural food, has less amounts of chlorine and nitrate group which are undesirous to a human body, is improved in palatability and preservability, and is useful in the field of food, food additives, drugs, or the like.

Abstract: An antioxidant substance which is a green leaf component in a green plant, comprising a component which is substantially insoluble in n-hexane but soluble in an aqueous ethanol solution having a water content of 0 to 80% by volume. The substance has an antioxidant activity as potent as or more potent than .alpha.-tocopherol, and is useful as an antioxidant for use in the field of foods, and medicines. Particularly, the antioxidant substance can be used for maintaining the freshness and quality of foods or storage thereof. The substance can be blended with cosmetics for skin and hair and are useful for the prevention of spots, freckles, chapping and sunburn.

Abstract: An antioxidant substance which is a green leaf component in a green plant, comprising a component which is substantially insoluble in n-hexane but soluble in an aqueous ethanol solution having a water content of 0 to 80% by volume. The substance has an antioxidant activity as potent as or more potent than .alpha.-tocopherol, and is useful as an antioxidant for use in the field of foods, and medicines. Particularly, the antioxidant substance can be used for maintaining the freshness and quality of foods or storage thereof. The substance can be blended with cosmetics for skin and hair and are useful for the prevention of spots, freckles, chapping and sunburning.

Abstract: A serum-free medium for cultivating a human monoclonal antibody-producing human/human hybridoma, said medium comprising a serum-free complete medium and at least 10.sup.-12 M but not more than 10.sup.-6 M of retinoic acid or its salt.

Abstract: A new human/human fused cell clone derived from human B cells having the ability to produce immunoglobulins and human B cells substantially lacking the ability to produce immunoglobulins, antigen-specific human immunoglobulins produced by the human/human fused cell clone, and a method of producing the human immunoglobulins. More specifically, a human/human hybridoma having the ability to produce antigen-specific human immunoglobulins, which is a human/human fused cell strain derived from human B cells of a human patient with liver cancer and a subclone of a human lymphoblast cell strain; and antigen-specific human immunoglobulins produced by the human/human fused cell strain.