Abstract: The present invention belongs to the field of biotechnology, and particularly relates to a Euglena culture medium and an application thereof. The Euglena culture medium includes the following components: NH4Cl, KH2PO4, MgSO4.7H2O, CaCl2.2H2O, Na2EDTA.2H2O, Fe2(SO4)3, CuSO4.5H2O, ZnSO4.7H2O, Co.(NH3).H2O, MnCl2.4H2O, vitamin B1 and vitamin B12. The pH is adjusted based on the Euglena culture medium of the present invention to achieve the purpose of preconcentrating Euglena cells. In the present invention, the Euglena cells are less ruptured, which improves the concentration efficiency of Euglena cells and reduces the treatment cost.