Abstract: The present disclosure relates to a composition for increasing the biological activity of stem cells using mixture 4F. Stem cells treated with mixture 4F according to the present disclosure not only acquire undifferentiated characteristics (stemness), but also have the advantage of improving cell proliferative ability and mobility, and thus, after being transplanted into a body, the stem cells can improve cell survival and engraftment and further enhance the ability to regenerate blood vessels and tissues. Accordingly, the stem cells can have various applications in the fields of stem cell differentiation and ischemic disease prevention or treatment.

Abstract: The present invention relates to a method for transdifferentiating cells. The present invention provides a method for transdifferentiating fibroblasts into chondrocytes, comprising: forming a micromass of fibroblasts by culturing fibroblasts in a high density; and applying an electrical stimulation such as a current or magnetic field to the micromass of fibroblasts while culturing the micromass of fibroblasts in a culture medium not containing growth factors.