Abstract: Systems for identifying the composition of bioaerosol particles using a TOFMS MALDI matrix-less system. A continuous timing laser triggers an IR ionization laser to fire when each particle enters the beam of the continuous trigger laser and is used to determine optical properties of the aerosol particles in association with one or more laser scattering devices and generate optical data. Ionized fragments produced when each particle is struck by the pulse ionization laser are analyzed using a TOFMS detector. A data analysis system is configured to compile the optical data with unique mass spectral data associated with each particle using data fusion and compare the compiled optical data with a training data set comprising of a knowledge base of known aerosol particles to predict composition.

Abstract: Systems and methods to provide rapid and autonomous detection of biological and chemical analyte particles in gas and liquid samples. Systems and methods for capturing and identifying biological and chemical aerosol analyte particles using matrix assisted laser desorption mass spectrometry (MALDI-MS) and using time-of-flight mass spectrometry (TOFMS) are disclosed. High specificity for capture and detection of aerosolized fentanyl was demonstrated using a portable sample capture and analysis system.

Abstract: Disclosed are methods and devices for analyzing aerosol particles in exhaled breath using diagnostic tools that enable rapid, low cost and autonomous point of care assays for several diseases including respiratory tract diseases. Disclosed are methods and devices for capturing exhaled breath aerosols in a packed bed column and analyzing exhaled captured breath aerosols for tuberculosis diagnosis.

Abstract: Systems and methods to provide rapid and autonomous detection of biological and chemical analyte particles in gas and liquid samples. Systems and methods for capturing and identifying biological and chemical aerosol analyte particles using matrix assisted laser desorption mass spectrometry (MALDI-MS) and using time-of-flight mass spectrometry (TOFMS) are disclosed. High specificity for capture and detection of aerosolized fentanyl was demonstrated using a portable sample capture and analysis system.

Abstract: Systems for identifying the composition of aerosol particles, particularly that of non-biological aerosol particles or biological aerosol particles including surface-bound water. A continuous timing laser triggers an IR ionization laser to fire when each particle enters the beam of the continuous trigger laser and determine optical properties of the aerosol particles in association with one or more laser scattering devices and generate optical data. The continuous laser beam and the pulse ionization laser beam are disposed as overlapping beams. Ionized fragments produced when each particle is struck by the ionization laser are analyzed using a TOFMS detector. A data analysis system is configured to compile the optical data with unique mass spectral data associated with each particle using data fusion and compare the compiled optical data with a training data set comprising of a knowledge base of known aerosol particles to predict composition.

Abstract: Systems are methods for identifying the composition of non-biological aerosol particles or biological aerosol particles including water bound to the surface of the particles, without pre-treatment using complex organic MALDI matrices. A continuous timing laser may be used to index the aerosol particles, determine optical particle properties, and trigger an IR pulse ionization laser. Ionized fragments, and optionally photons, associated with each particle produced by ionization of the particles the IR ionization laser is analyzed using one or more detectors including a TOFMS detector and an optical detector. Unique mass spectral data and optical data associated with each indexed particle is compiled using data fusion to generate a compiled data set. The compiled optical data is compared with a training data set comprising a knowledge base of known aerosol particles to predict aerosol particle composition.

Abstract: In-vehicle occupant detection system and methods capable of monitoring the interior of a passenger vehicle and responding to potentially dangerous situations are disclosed. The detection systems may comprise a front camera to focus on driver and passenger behavior in the front cabin, and a rear camera and an array of environmental sensors to detect unattended occupants including children, adults or pets in the rear seats. Data may be processed by an intelligent algorithm to assesses anomalies and trigger one or more alarms or countermeasures.

Abstract: Disclosed are methods and devices for analyzing aerosol particles in exhaled breath using diagnostic tools that enable rapid, low cost and autonomous point of care assays for several diseases including respiratory tract diseases. Disclosed are methods and devices for capturing exhaled breath aerosols in a packed bed column and analyzing exhaled captured breath aerosols for tuberculosis diagnosis.

Abstract: Disclosed are systems are methods for identifying the composition of single aerosol particles, particularly that of bioaerosol particles. A continuous timing laser tightly coupled with a pulse ionization laser is used to index aerosol particles, measure particle properties, and trigger the ionization laser to fire when each particle enters the beam of the trigger laser. Ionized fragments and optionally photons produced when each particle is struck by the ionization laser are analyzed using one or more detectors including a TOF-MS detector and an optical detector. Individual single particle spectra are aligned and denoised prior to averaging.

Abstract: Disclosed are systems are methods for identifying the composition of single aerosol particles, particularly that of bioaerosol particles, without pre-treatment using complex organic MALDI matrices. A continuous timing laser may be used to index aerosol particles, measure particle properties, and trigger a pulse ionization laser. Ionized fragments and optionally photons associated with each particle producing by the ionization laser may be analyzed using one or more detectors including a TOF-MS detector and an optical detector. The laser pulse may comprise a simultaneous IR and UV laser pulse when fragments comprise predominantly of UV chromophores. Unique spectral data associated with each indexed particle from each detector may be compiled using data fusion to generate compiled spectral data. Machine learning methods may be used to improve the prediction of composition over time.

Abstract: Methods and devices for capturing and analyzing aerosolized particles in exhaled breath characteristic of a respiratory disease to enable rapid, low-cost point of care assays for several diseases including respiratory tract diseases such as COVID-19 are disclosed. The disclosed methods and systems selectively capture aerosolized particles using a packed bed column. The captured particles are then eluted using solvents and analyzed using analytical devices including MALDI-TOFMS.

Abstract: Methods and devices for analyzing non-volatile organics in exhaled breath and other aerosols using various diagnostic tools that enable rapid, low-cost point of care assays for several diseases including respiratory tract diseases such as COVID-19. The disclosed methods and systems selectively capture non-volatile organics in exhaled breath and other aerosols in a packed bed column. The non-volatile organics are eluted and samples are analysis using diagnostic devices including MALDI-TOFMS. The disclosed systems and methods provide for a diagnostic test result in less than about 20 minutes and provides for autonomous operation with minimal human intervention.

Abstract: Mobile decontamination systems and microwave assisted decontamination methods for decontaminating a variety of contaminated surfaces disposed external to the system. The systems and methods include treating the surfaces with benign chemical formulations followed by exposing to microwave irradiation for short periods of time to achieve at least 6-log reduction in biological contaminants including viruses and spores of B. anthracis, B. thuringiensis, and P. roqueforti. The formulations include a percarbonate and surfactant in water.

Abstract: Mobile decontamination systems and microwave assisted decontamination methods for decontaminating a variety of contaminated surfaces disposed external to the system are disclosed. The systems and methods comprise treating the surfaces with benign chemical formulations followed by exposing to microwave irradiation for short periods of time to achieve at least 6-log reduction in biological contaminants including viruses and spores of B. anthracis, B. thuringiensis, and P. roqueforti. The formulations comprise a percarbonate and surfactant in water.

Abstract: Methods and devices to capture and analyze aerosolized particles in exhaled air including protein biomarkers and their truncated proteoforms characteristic of tuberculosis to enable rapid detection of diseases. Methods and systems to selectively capture aerosolized particles using a packed bed column. The captured particles are eluted using one or more solvents and analyzed using devices including MALDI-TOFMS.

Abstract: Disclosed are microwave assisted methods and systems for decontaminating a variety of contaminated surfaces. The systems and methods comprise treating the surfaces with benign chemical formulations followed by exposing to microwave irradiation for short periods of time to achieve at least 6-log reduction in biological contaminants including spores of B. anthracis, B. thuringiensis, and P. roqueforti. Chemical formulations may comprise copper (II) chloride in water. The formulations may include a surfactant such as a polyethylene sorbitol ester surfactant.

Abstract: Disclosed are methods and devices for analyzing non-volatile organics in exhaled breath and other aerosols using various diagnostic tools that enable rapid, low cost point of care assays for several diseases including respiratory tract diseases such as COVID-19. The disclosed methods and systems selectively capture non-volatile organics in exhaled breath and other aerosols in a packed bed column. The non-volatile organics are eluted and samples are analysis using diagnostic devices including MALDI-TOFMS. The disclosed systems and methods provide for a diagnostic test result in less than about 20 minutes and provides for autonomous operation with minimal human intervention.

Abstract: Systems and methods to provide rapid and autonomous detection of biological and chemical analyte particles in gas and liquid samples. Systems and methods for capturing and identifying biological and chemical aerosol analyte particles using matrix assisted laser desorption mass spectrometry (MALDI-MS) and using time-of-flight mass spectrometry (TOFMS) are disclosed. High specificity for capture and detection of aerosolized fentanyl was demonstrated using a portable sample capture and analysis system.

Abstract: Disclosed are methods and devices for analyzing non-volatile organics in exhaled breath and other aerosols using various diagnostic tools that enable rapid, low cost point of care assays for several diseases including respiratory tract diseases such as COVID-19. The disclosed methods and systems selectively capture non-volatile organics in exhaled breath and other aerosols in a packed bed column. The non-volatile organics are eluted and samples are analysis using diagnostic devices including MALDI-TOFMS. The disclosed systems and methods provide for a diagnostic test result in less than about 20 minutes and provides for autonomous operation with minimal human intervention.

Abstract: Disclosed are systems and methods to provide rapid and autonomous detection of analyte particles in gas and liquid samples. Disclosed are methods and devices for identifying biological aerosol analytes using MALDI-MS and chemical aerosol analytes using LDI and MALDI-MS using time-of-flight mass spectrometry (TOFMS).