Abstract: The present invention relates to the technical field of porcine pseudorabies virus, and more particularly to a porcine pseudorabies virus (PRV)-YF strain, with the preservation No. of CCTCC No. V201502. The present invention also provides an application of the porcine pseudorabies virus (PRV)-YF strain in preparing an inactivated vaccine of the porcine pseudorabies virus, the inactivated vaccine of the porcine pseudorabies virus and a preparation method thereof. It is proved through an immune efficacy evaluating test and a safety test that the inactivated vaccine of the porcine pseudorabies virus has good immune protective efficacy and is safe for weaned piglets, replacement gilts, pregnant sows and the like.

Abstract: The invention discloses an avian infectious bronchitis virus purification method. The method uses ordinary avian embryo which was affected by avian bronchitis virus. Using the combination of centrifugation, ultrafiltration, concentration, molecular sieve chromatography and ultrafiltration method can effectively remove miscellaneous protein in embryo-derived infectious bronchitis virus antigen, high virus recovery, and have no effect on the activity of avian infectious bronchitis virus. The avian infectious bronchitis virus purified by this method can be directly used in the preparation of avian infectious bronchitis virus vaccine.