Abstract: The present invention discloses an endophytic fungal strain Pseudophialophora sp. P-B313 and an application thereof, and belongs to the technical filed of microbial applications. The deposit number of the endophytic fungal strain P-B313 is CCTCC M 2021504, and the scientific name thereof is Pseudophialophora sp. The endophytic fungal strain P-B313 can enhance the resistance of rice against seedling leaf blast with a control efficiency of 87.56% and a disease index reduced by 62.59. The biological control efficiency of the endophytic fungus P-B313 against seedling leaf blast in rice has great value by promotion and applications thereof in the field of agriculture.

Abstract: The present invention discloses an application of an endophytic fungi FO-R20 in prevention and treatment of rice neck or panicle blast, and belongs to the technical filed of microbial applications. The Falciphora oryzae FO-R20 with a deposit number of CCTCC M 2021505 is prepared into a seed coating agent or solid fungal fertilizer and applied to rice seeds; and in the culture process, the strain is colonized at the roots of rice, thus the disease resistance of rice to neck or panicle blast is remarkably enhanced, the prevention and treatment efficiency reaches 86.91%-90.85%, and the disease index is reduced by 64.92-72.07. The biological prevention and treatment effect of the endophytic fungi Falciphora oryzae FO-R20 against rice neck or panicle blast has great value by promotion and applications thereof in the field of agriculture.

Abstract: A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.

Abstract: A spatial prediction method of rice stable isotope based on environmental similarity is provided. The method comprises: describing environmental characteristics of the rice stable isotope; measuring an environmental similarity between a site to be predicted and a sample site; measuring a reliability of the sample site; and carrying out a spatial prediction on the rice stable isotope according to the environmental similarity between the site to be predicted and the sample site and the reliability of the sample site. This method can improve the accuracy of the prediction result.

Abstract: A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.

Abstract: The present invention relates to a rice seedling substrate and a preparation method thereof, which is of the technical field of rice planting. The rice seedling substrate according to the invention was prepared from the raw materials includes the following by volume: 40-60% of dredging sediment, 30-50% of vegetable straw, and 10-30% of hickory shell. The rice seedling substrate according to the invention can not only turn waste into treasure to improve the recycling rate of sediment and agricultural and forestry waste resources, but also save the limited cultivated soils, reduce the production cost of rice seedling substrate, and promote the healthy and sustainable development of rice planting. Therefore, The inventive substrate has significant benefits in terms of economics, sociology and ecology.

Abstract: The present invention provides a set of large-scale and high-efficiency fly maggot culture equipment and a large-scale and high-efficiency fly maggot culture process and pertains to fly maggot culture. The equipment comprises at least one culturing workshop with a multi-functional ceiling. At least one feeder traveling along double-row burden distributing rails is arranged in the culturing workshop. A pair of main rails is perpendicularly arranged on the front of one ends of the burden distributing rails. A rail car used for transferring the feeder among different burden distributing rails is arranged on the main rails. Transferring rails horizontally perpendicular to the main rails are arranged on the rails car. The height of the transferring rails is consistent with the height of the distributing rails. The workshop ground between the two rails of the distributing rails form a culturing bed for fly maggot culture.

Abstract: The present invention discloses a method of preparing a functional peptide from a germinated bean, including the steps of: germinating a bean until a sprout length is about 0.5-1.0 cm; adding water to the obtained germinated bean, and then blending and crushing the germinated bean; adjusting the obtained slurry to a pH value between 6.5-8.0, and then adding neutral protease, papain and compound flavor protease to perform enzymatic reaction under stirring condition, wherein an enzymatic reaction solution is obtained including the functional peptide; subjecting the enzymatic reaction solution to ultrafiltration using an ultrafiltration membrane; keeping the ultrafiltrated enzymatic reaction solution at a temperature between 70-80° C. or using ozonated water at a concentration between 20-25 mg/L thereby avoiding decomposition by microorganisms; and performing spray drying to obtain the functional peptide.

Abstract: A method for altering the ratio of proteins/lipids in crop seeds utilizing the antisense gene, comprising the steps of: (1) cloning the gene for the phosphoenopyruvate carboxylase or acetyl-CoA carboxylase, or the fragment thereof; (2) constructing the corresponding antigene; (3) introducing the said antigene into the cells of the rapeseeds, so that the activity of the phosphoenopyruvate carboxylase or the acetyl-CoA carboxylase being important for the protein synthesis is changed, the flow direction of the common substrate, PEP, can be controlled, so that the proteins/lipids in the seeds is altered. The application can be used to alter the proteins/lipids in the crop seeds.