Patents Assigned to ZHEJIANG DSM ZHONGKEN BIOTECHNOLOGY CO., LTD.
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Patent number: 11149293Abstract: This invention provides a fermentation method of producing gellan gum, wherein a certain amount of fermentation broth is retained in the fermentor as a seed for next batch fermentation or transferred to another fermentor as a seed for next batch fermentation in that fermentor. The fermentation method of this invention reduces fermentation cost of gellan gum and lowers contamination risk during seed cultivation.Type: GrantFiled: November 1, 2018Date of Patent: October 19, 2021Assignees: Zhejiang DSM Zhongken Biotechnology Co. Ltd, DSM IP Assets B.V.Inventors: Jie Zhao, Wouter Adrianus Van Winden, Rogier Meulenberg, Zhihui Gao
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Patent number: 8864994Abstract: Disclosed is a method for extracting high acyl gellan from the fermentation broth containing gellan gum with a low production cost and high quality of products, comprising the following steps: (1) Treatment of the fermentation broth with an enzyme; (2) flocculation of the treated fermentation broth with acid; (3) wash of the fiber-like material; and (4) drying and milling.Type: GrantFiled: June 2, 2010Date of Patent: October 21, 2014Assignee: Zhejiang DSM Zhongken Biotechnology Co., Ltd.Inventors: Xuegang Wang, Rongming Wu, Zhiming Zhang, Jialiang Wang, Huaiyuan Xu, Yubin Shen, Liqiang Yang
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Patent number: 8685698Abstract: A yellow pigments generation deficient Sphingomonas strain (Sphingomonas sp. ZD001) and application thereof in preparing gellan gum by microbial fermentation are provided. The strain is preserved in China Center for Type Culture Collection (CCTCC) with the address of Wuhan University, Wuhan, 430072, China, the preservation date of the strain is 10 Sep. 2009, and the preservation serial number is CCTCC No: M 209298. The main beneficial effect of the present invention is that the fermented liquor contains no yellow pigments but is milky white, colorless superior gellan gum can be obtained by only depositing polysaccharide with a small quantity of ethanol or isopropanol, so that the post purification and de-coloration techniques of gellan gum production can be simplified, the yield can be enhanced, and the production cost can be reduced.Type: GrantFiled: August 13, 2010Date of Patent: April 1, 2014Assignees: Zhejiang University, Zhejiang DSM Zhongken Biotechnology Co., Ltd.Inventors: Xuechang Wu, Rongming Wu, Ou Li, Liang Zhu, Yamin Chen, Chaodong Qian, Mei Chen
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Patent number: 8652549Abstract: The invention relates to a post-extraction process for preparing low acyl gellan gum having high solubility property, the process comprising the following steps: (1) enzyme treatment of fermentation broth; (2) flocculation of fermentation broth; (3) deacylation treatment; (4) clarification treatment of deacylated solution; (5) dehydration treatment of clarified and deacylated gellan gum solution; (6) ion exchange and decoloration treatment; and (7) drying and milling. The process according to the invention produces a product, which has largely improved solubility, largely improved quality, good appearance, high transparence, and high gel strength.Type: GrantFiled: June 2, 2010Date of Patent: February 18, 2014Assignee: Zhejiang DSM Zhongken Biotechnology Co., Ltd.Inventors: Rongming Wu, Yanfei Zhong, Qi Guo, Yueqiang Shen, Liping Shen
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Patent number: 8609377Abstract: The present invention relates to a post extraction process for preparing low acyl gellan, comprising the steps: deacylation treatment of gellan gum-containing fermentation broth, enzyme treatment, flocculation of low acyl gellan gum with divalent or polyvalent metal cations, clarification treatment of gellan gum solution, dehydration treatment of gellan gum solution, removal of divalent or polyvalent cations and decoloration, and drying and milling. Preferably, the process comprises the step of formulating a proper amount of chelating agent/acid system before the drying and milling step to chelate the added additional divalent cations added during the use of gellan gum, and at the same time to keep the pH value in a relatively stable condition. The present invention also provides the various low acyl gellan gums prepared by the above methods. The product has the following characteristics: a good appearance, a high transmittancy, and high gel strength.Type: GrantFiled: June 2, 2010Date of Patent: December 17, 2013Assignee: Zhejiang DSM Zhongken Biotechnology Co., Ltd.Inventors: Baoyi Yang, Binghua Wu, Huaiyuan Xu, Zhengxue Zhu, Yubin Shen, Xuegang Wang, Liqiang Yang, Jialiang Wang, Wenhui Jiang, Shengqiang Bao, Xiaolin Sheng, Xiaoqin Shen
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Publication number: 20110281307Abstract: The present invention relates to a post extraction process for preparing low acyl gellan, comprising the steps: deacylation treatment of gellan gum-containing fermentation broth, enzyme treatment, flocculation of low acyl gellan gum with divalent or polyvalent metal cations, clarification treatment of gellan gum solution, dehydration treatment of gellan gum solution, removal of divalent or polyvalent cations and decoloration, and drying and milling. Preferably, the process comprises the step of formulating a proper amount of chelating agent/acid system before the drying and milling step to chelate the added additional divalent cations added during the use of gellan gum, and at the same time to keep the pH value in a relatively stable condition. The present invention also provides the various low acyl gellan gums prepared by the above methods. The product has the following characteristics: a good appearance, a high transmittancy, and high gel strength.Type: ApplicationFiled: June 2, 2010Publication date: November 17, 2011Applicant: Zhejiang DSM Zhongken Biotechnology Co., Ltd.Inventors: Baoyi Yang, Binghua Wu, Huaiyuan Xu, Zhengxue Zhu, Yubin Shen, Xuegang Wang, Liqiang Yang, Jialiang Wang, Wenhui Jiang, Shengqiang Bao, Xiaolin Sheng, Xiaoqin Shen
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Publication number: 20110281308Abstract: A yellow pigments generation deficient Sphingomonas strain (Sphingomonas sp. ZD001) and application thereof in preparing gellan gum by microbial fermentation are provided. The strain is preserved in China Center for Type Culture Collection (CCTCC) with the address of Wuhan University, Wuhan, 430072, China, the preservation date of the strain is 10 Sep. 2009, and the preservation serial number is CCTCC No: M 209298. The main beneficial effect of the present invention is that the fermented liquor contains no yellow pigments but is milky white, colorless superior gellan gum can be obtained by only depositing polysaccharide with a small quantity of ethanol or isopropanol, so that the post purification and de-coloration techniques of gellan gum production can be simplified, the yield can be enhanced, and the production cost can be reduced.Type: ApplicationFiled: August 13, 2010Publication date: November 17, 2011Applicants: Zhejiang DSM Zhongken Biotechnology Co., Ltd., Zhejiang UniversityInventors: Xuechang Wu, Rongming Wu, Ou Li, Liang Zhu, Yamin Chen, Chaodong Qian, Mei Chen
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Publication number: 20110269189Abstract: Disclosed is a method for extracting high acyl gellan from the fermentation broth containing gellan gum with a low production cost and high quality of products, comprising the following steps: (1) Treatment of the fermentation broth with an enzyme; (2) flocculation of the treated fermentation broth with acid; (3) wash of the fiber-like material; and (4) drying and milling.Type: ApplicationFiled: June 2, 2010Publication date: November 3, 2011Applicant: Zhejiang DSM Zhongken Biotechnology Co Ltd.Inventors: Xuegang Wang, Rongming Wu, Zhiming Zhang, Jialiang Wang, Huaiyuan Xu, Yubin Shen, Liqiang Yang
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Publication number: 20110268843Abstract: The invention relates to a post-extraction process for preparing low acyl gellan gum having high solubility property, the process comprising the following steps: (1) enzyme treatment of fermentation broth; (2) flocculation of fermentation broth; (3) deacylation treatment; (4) clarification treatment of deacylated solution; (5) dehydration treatment of clarified and deacylated gellan gum solution; (6) ion exchange and decoloration treatment; and (7) drying and milling. The process according to the invention produces a product, which has largely improved solubility, largely improved quality, good appearance, high transparence, and high gel strength.Type: ApplicationFiled: June 2, 2010Publication date: November 3, 2011Applicant: ZHEJIANG DSM ZHONGKEN BIOTECHNOLOGY CO., LTD.Inventors: Rongming Wu, Yanfei Zhong, Qi Guo, Yueqiang Shen, Liping Shen