Abstract: The present invention discloses a method for synthesizing 25-hydroxy cholesterol. The method is as below: subjecting 24-dehydrocholesterol derivative as a raw material, which undergoes an addition reaction with a hydroxyl containing reagent in an organic solvent under catalysis, and then hydrolyzing the reaction product and separating to obtain 25-hydroxy cholesterol. The present invention adopts hydroxyl containing reagents such as water, formic acid, acetic acid, propionic acid, butyric acid, benzoic acid, p-methyl benzoic acid to replace the commonly used extremely toxic reagents such as Cr reagent, Hg reagent and polyfluorinated acetone in the prior art. The raw materials of the present invention are easily available, and have low effect on environment. The operation and post treatment are convenient.
Type:
Grant
Filed:
May 22, 2015
Date of Patent:
October 31, 2017
Assignees:
ZHEJIANG UNIVERSITY OF TECHNOLOGY, ZHEJIANG GARDEN BIOCHEMICAL HIGH-TECH CO., LTD, HANGZHOU XIASHA BIOCHEMICAL TECH CO., LTD
Inventors:
Can Jin, Weike Su, Ziqiang Wang, Jiangang Liu, Bin Sun, Wenhao Xu
Abstract: The present invention discloses a method for synthesizing 25-hydroxy cholesterol. The method is as below: subjecting 24-dehydrocholesterol derivative as a raw material, which undergoes an addition reaction with a hydroxyl containing reagent in an organic solvent under catalysis, and then hydrolyzing the reaction product and separating to obtain 25-hydroxy cholesterol. The present invention adopts hydroxyl containing reagents such as water, formic acid, acetic acid, propionic acid, butyric acid, benzoic acid, p-methyl benzoic acid to replace the commonly used extremely toxic reagents such as Cr reagent, Hg reagent and polyfluorinated acetone in the prior art. The raw materials of the present invention are easily available, and have low effect on environment. The operation and post treatment are convenient.
Type:
Application
Filed:
May 22, 2015
Publication date:
June 8, 2017
Applicants:
Zhejiang University of Technology, Zhejiang Garden Biochemical High-Tech Co., Ltd, Hangzhou Xiasha Biochemical Tech Co., Ltd
Inventors:
Can JIN, Weike SU, Ziqiang WANG, Jiangang LIU, Bin SUN, Wenhao XU