Abstract: Provided is a new method for telomerase amplification—washing-free anchored-extension and telomeric-binding amplification (WATA). The method comprises: utilizing an anchored telomerase primer to conduct telomere TTAGGGG sequence (“G sequence”) extension; hybridizing with a template probe having a universal PCR primer sequence and six units of telomere CCCTAA sequence (“C sequence”) on the extended G sequence; removing the uncombined template probe via an enzyme digestion reaction; and the template probe combined with the G sequence conducts PCR reaction, the amplification product being a peculiar DNA fragment of a fixed length.

Abstract: Provided in the present invention are an affinity mediated amplification method of telomeric C-ssDNA with a template probe and a detection kit using this method for performing alternative lengthening detection of telomeres.

Abstract: Provided in the present invention is a washing-free template-ready PCR detection method for RNA. On the basis of retaining the advantages of the original template-ready PCR method, i.e., there being no need to purify and extract the RNA, no need for a reverse transcription reaction, etc., the method of the present invention designs a probe for the restriction enzymes to thereby integrate the enzyme digestion reaction, so as to eliminate the interference of various pollution sources of double-stranded DNA with no need for a washing step.