Abstract: The present invention discloses Lactobacillus sakei for alleviating allergy and a preparation method thereof, and particularly Lactobacillus sakei for alleviating wheat-dependent, exercise-induced anaphylaxis (WDEIA). The Lactobacillus sakei is classified and named as Lactobacillus sakei. The Lactobacillus sakei has been preserved in China Center for Type Culture Collection since Apr. 12, 2003, with a preservation number of CCTCC DB 20082413. The Lactobacillus sakei can regulate the immune function of a human body to prevent/treat WDEIA by reducing the contents of specific IgE and histamine in serum of WDEIA patients.