Abstract: The present invention discloses a ?-transaminase mutant obtained through DNA synthetic shuffling combined mutation. The ?-transaminase mutant is obtained through point mutation of a wild type ?-transaminase from Aspergillus terrus. The amino acid sequence of the wild type ?-transaminase is shown in SEQ ID NO: 1. The mutation site of the ?-transaminase mutant is any one of: (1) F115L-H210N-M150C-M280C; (2) F115L-H210N; (3) F115L-H210N-E253A-I295V; (4) I77L-F115L-E133A-H210N-N245D; (5) I77L-Q97E-F115L-L118T-E253A-G292D; (6) I77L-E133A-N245D-G292D; and (7) H210N-N245D-E253A-G292D. According to the present invention, forward mutations obtained in the previous stage are randomly combined through a DNA synthetic shuffling combined mutation method.

Abstract: The present disclosure discloses a Lactobacillus plantarum ZUST49 and a bacterial agent, and use thereof, and belongs to the technical field of microorganisms. An aspect of the present disclosure provides a Lactobacillus plantarum ZUST49 and a bacterial agent containing the Lactobacillus plantarum ZUST49, and another aspect provides use of the Lactobacillus plantarum ZUST49 and the bacterial agent containing the Lactobacillus plantarum ZUST49. The Lactobacillus plantarum ZUST49 of the present disclosure may convert glucoraphanin into sulforaphane, especially in a human or animal intestinal environment. After an enteric probiotic preparation prepared from the strain of the present disclosure is orally taken, the Lactobacillus plantarum ZUST49 may efficiently convert the glucoraphanin into the sulforaphane in an intestinal tract, which not only solves a problem that stability of the sulforaphane cannot be improved in the prior art, but also effectively exerts a probiotic effect.

Abstract: The method of the present invention specifically comprising following steps: adding a chitosan to an acetic acid solution, heating and stirring until clear, to obtain an acidic chitosan solution; then adding a palm wax and heating a resulting mixture to 80-100° C. with a constant temperature and a high-speed stirring for emulsification for 5-15 minutes; after the emulsification is completed, an obtained emulsion is quickly cooled to a room temperature. The present invention uses a single-layer coating method to prepare waterproof and oil-proof food cardboard, simplifying operation and achieving good waterproof, oil-proof, and antibacterial effects with a lower coating amount, thereby reducing production costs. When a coating amount is 5-10 g/m2, a Cobb60 value can reach 5-9 g/m2, an oil resistance level can reach 11-12 levels, a water contact angle can reach over 130°, and an antibacterial rate can reach over 90%.

Abstract: The present invention belongs to the technical field of electric automobiles and particularly relates to a fault tolerance decision-making method and system for sensor failure of a vehicular wheel hub driving system. The method comprises a current sensor failure diagnostic process, a position/velocity sensor failure diagnostic process and a selection process for a wheel hub motor fault tolerance control method. The system comprises a current sensor failure diagnostic module, a position/velocity sensor failure diagnostic module and a selection module for a wheel hub motor fault tolerance control method. The position/velocity sensor failure diagnostic module further comprises a fault tolerance control switching module.

Abstract: Disclosed is a preparation method of double-layer carbon-paper base paper with high uniformity and gradient pores, including adding carbon fibers to alkali lignin aqueous solution for treatment, controlling the alkali lignin aqueous solution to reach subcritical water conditions, and modifying the carbon fibers by sodium phenol reactive groups under the subcritical water conditions of the alkali lignin, for improving hydrophilic groups on a surface of the carbon fibers and a surface rough structure; further, modifying the carbon fibers to produce carbon nanotube clusters on the surface of the carbon fibers to obtain surface-thermally-modified carbon fibers, and adding the surface-thermally-modified carbon fibers to a dispersant for dispersion, stirring together with auxiliary materials to prepare a mixed slurry, and preparing the mixed slurry into the carbon-paper base paper by wet forming process.

Abstract: The present invention relates to a method for moderately increasing the content of dimethyl disulfide in casein-containing processed cheese. The method includes the following steps: taking a cheese powder, adding chymosin for enzymolysis, and subjecting a material obtained after the enzymolysis to puffing and drying to obtain a modified cheese powder; preparing materials; taking the modified cheese powder, heating the modified cheese powder under stirring until the cheese powder is melt, adding sodium bicarbonate to adjust the pH to 6-7, and adding papain for enzymolysis; taking a material obtained after the enzymolysis, adding butter, a skimmed milk powder, fructo-oligosaccharide and water for mixing by stirring, and then adding an emulsifying salt, glutamine transaminase and salt for uniform heating and stirring to obtain a mixture; and subjecting the mixture to heat treatment, hot filling and cooling in sequence to obtain processed cheese.

Abstract: The present invention relates to a method for moderately increasing the content of dimethyl disulfide in casein-containing processed cheese. The method includes the following steps: taking a cheese powder, adding chymosin for enzymolysis, and subjecting a material obtained after the enzymolysis to puffing and drying to obtain a modified cheese powder; preparing materials; taking the modified cheese powder, heating the modified cheese powder under stirring until the cheese powder is melt, adding sodium bicarbonate to adjust the pH to 6-7, and adding papain for enzymolysis; taking a material obtained after the enzymolysis, adding butter, a skimmed milk powder, fructo-oligosaccharide and water for mixing by stirring, and then adding an emulsifying salt, glutamine transaminase and salt for uniform heating and stirring to obtain a mixture; and subjecting the mixture to heat treatment, hot filling and cooling in sequence to obtain processed cheese.

Abstract: The present application is related to a method for preparing a feeding instant Astragalus polysaccharide powder and an application thereof. The method includes the following steps: adding deionized water at 65° C. to a dreg of Astragalus membranaceus by water extraction and alcohol precipitation, controlling an alcohol volume fraction at 20%, stirring fully to dissolve the dreg, and after a dreg solution cools to room temperature, removing insoluble thermo-sensitive macromolecular proteins, polysaccharides and solid residues by centrifugation at 3500 revolutions per minute to obtain a clear supernatant. Ultrafiltration treatment is performed by hollow fiber membranes on the supernatant obtained after the centrifugation, and 10-100 kDa polysaccharide components are collected. A low-molecular-weight instant Astragalus polysaccharide powder with high immunocompetence is obtained through an interlayer cold air cooling spray drying process, and the content of active polysaccharide is more than 87%.

Abstract: A polishing device for a metal conveyor belt suitable for an automatic conveyor line includes a holding pillar with one end fixedly provided with a bearing head, a supporting carriage fixedly connected to the bearing head, assembly supporting frames, an assembly sliding column, a polishing supporting shaft and locking mechanism. A bottom end of the holding pillar is provided with a barrier protection assembly. Both ends of the supporting carriage are respectively provided with a reciprocating assembly and two assembly supporting frames. Opposite side surfaces of the two assembly supporting frames are respectively rotatably connected with a rotating seats. Both ends of the assembly sliding column are respectively fixedly installed and in transmission connection with two rotating seats. Both ends of the assembly sliding column are respectively in transmission connection with the reciprocating assembly. The polishing supporting shaft is in transmission connection with the assembly sliding column.

Abstract: Disclosed is an environmentally-friendly method for enhancing mechanical properties of a high antibacterial nano dialysis paper for medical protection, includes following steps: a, preparing pulp components, and taking 40-60 parts of hardwood pulp, 10-20 parts of polyvinyl alcohol fiber, 30-50 parts of softwood pulp, 10-20 parts of cellulose fiber by mass; b, putting the hardwood pulp, the polyvinyl alcohol fiber, the softwood pulp and the cellulose fiber into a beater for beating to make a pulp, and then adding modified chitosan accounting for 1-5% of a weight of the pulp into the pulp, and defibering for 10-20 minutes at a rotating speed of 1500-2000 revolutions per minute in a defibrator; and c, carrying out papermaking with the pulp after defibering to form a dialysis paper finished product.

Abstract: A one-step method for preparing a magnetic magnesium-iron layered double hydroxide (LDH)-biochar composite material and use thereof provided. Biomass, as a substrate, is placed in a ferric salt solution, magnesium hydroxide is added, the materials are fully stirred and aged for a certain time, the aged materials are dried to obtain a magnesium-iron LDH-biomass, and the magnetic magnesium-iron LDH-biochar composite material is obtained after pyrolysis. The method solves problems of uncontrollable reaction and low crystallinity of products in preparing LDH using a coprecipitation method, reduces the amount of drugs, omits a step of adjusting a pH with an alkaline solution, improves yield and reduces a cost. The obtained magnetic magnesium-iron LDH-biochar composite material exhibits an excellent performance in adsorbing phosphate in water and can be recovered by an external magnetic field. Thus, an important method is provided for preparing LDH and the composite thereof.

Abstract: A preparation method of charcoal-based fertilizer is provided. Particularly, a special pig manure charcoal modified by amino grafting, a preparation method thereof, and its application in the reuse of nitrogen from farmland drainage are provided. The preparation method includes the following steps: 1) drying raw pig manure to a moisture content of 80%-85% and carrying out pickling, drying, and crushing successively to obtain a dried pig manure powder; 2) conducting liquid nitrogen pretreatment and high-temperature charcoalization to obtain an expanded pig manure charcoal; 3) performing carboxylation treatment to obtain a carboxylated pig manure charcoal; 4) amino grafting: adding an ammonia liquor to the carboxylated pig manure charcoal obtained in step 3), stirring for 20-24 h in an oil bath at 200-240° C.; washing and filtering; and drying and grinding to obtain the special pig manure charcoal modified by amino grafting.

Abstract: A preparation method of charcoal-based fertilizer is provided. Particularly, a special pig manure charcoal modified by amino grafting, a preparation method thereof, and its application in the reuse of nitrogen from farmland drainage are provided. The preparation method includes the following steps: 1) drying raw pig manure to a moisture content of 80%-85% and carrying out pickling, drying, and crushing successively to obtain a dried pig manure powder; 2) conducting liquid nitrogen pretreatment and high-temperature charcoalization to obtain an expanded pig manure charcoal; 3) performing carboxylation treatment to obtain a carboxylated pig manure charcoal; 4) amino grafting: adding an ammonia liquor to the carboxylated pig manure charcoal obtained in step 3), stirring for 20-24 h in an oil bath at 200-240° C.; washing and filtering; and drying and grinding to obtain the special pig manure charcoal modified by amino grafting.

Abstract: A device and method for preparing a solidified cohesionless soil specimen for triaxial test. The device includes a first vessel for storing a grout, a first peristaltic pump, a grouting pipe, a first electrode rod, a direct-current power supply, a first glass stopper, a PMMA pipe, a circumferential grouting cylinder, a first hoop sleevedly provided on the circumferential grouting cylinder, a second hoop sleevedly provided on the PMMA pipe, a return pipe, a second glass stopper, a second electrode rod, a liquid outlet pipe, a first water-stop clamp, a second water-stop clamp, a second vessel for collecting an exudate, and a second peristaltic pump.

Abstract: An information interaction system and method for heterogeneous detection and recognition devices for a low-altitude unmanned aerial vehicle (UAV) are provided, where each edge data unit is connected to a uniquely corresponding low-altitude UAV detection and sensing device and an access and distribution unit. The access and distribution unit is connected to a storage service unit and at least one scheduling and calculation unit. Each scheduling and calculation unit is connected to a comprehensive calculation and display unit. The comprehensive calculation and display unit is connected to the storage service unit. The storage service unit sends historical data to the comprehensive calculation and display unit after being authorized. The method is applied to the information interaction system for heterogeneous detection and recognition devices for a low-altitude UAV. A problem of data collaborative application of different low-altitude UAV detection devices is resolved.

Abstract: A device for measuring a scalar magnetic field based on pulsed optical pumping, including: a first laser, a first isolator, a first ?/2 waveplate, a first polarizing beam splitter, a first coupler, a first spectrum analyzer, a reflector, an atomic sensor probe, a second ?/2 waveplate, a second polarizing beam splitter, a first photoelectric converter, a second photoelectric converter, a data acquisition system, a second laser, a second isolator, an acousto-optic modulator, a third ?/2 waveplate, a third polarizing beam splitter, a second fiber coupler, a second spectrum analyzer, a ?/4 waveplate and a beam expander. Two planar reflectors are respectively arranged at two sides of the atomic sensor probe and face towards the atomic sensor probe, and are arranged parallel to the pump beam input to the atomic sensor probe.

Abstract: Disclosed is a method of preparing a carbon quantum dot from soybean dregs by hydrothermal synthesis, including: mixing the soybean dregs with water, and subjecting the reaction mixture under heating at 100-500° C. in an autoclave for reaction; cooling the reaction mixture when the reaction is completed, and removing insoluble substances from the reaction mixture; and dialyzing the reaction mixture, and lyophilizing the dialyzed product to produce the carbon quantum dot. The invention further provides the carbon quantum dot prepared by the above method and a use thereof in the detection of Fe3+ and Hg2+ in water, where the lowest detection limit can reach 30 nmol/L and the detection range is 0.1-50 ?mol/L.

Abstract: A preparation method of a cross-linked sodium hyaluronate gel is disclosed, which including: preparing an alkaline aqueous solution of hyaluronic acid: formulating a sodium hyaluronate alkali liquor with the concentration of 10-30% g/ml; and carrying out a cross-linking reaction: the cross-linking agent used in the cross-linking reaction being divinyl sulfone or 1,4-butanediol diglycidyl ether, the cross-linking reaction being carried out in an alkaline aqueous solution of hyaluronic acid, the reaction temperature of the cross-linking reaction being 20-40° C., the time of the cross-linking reaction being 4-8 h, and the like. The method of this invention has many advantages, such as easily available raw materials, mild reaction conditions, high cross-linking efficiency, simple process and post-treatment, and easy operation.

Abstract: A high-concentration anaerobic fermentation apparatus includes a liquid accumulation tank, a fermentation vessel, a feeding assembly, and a biogas residue collecting bin. The feeding assembly includes a hopper and a return conveyor belt including a first portion extending downward from the hopper till below a liquid level, a second portion extending upward from a bottom end of the first portion till above the liquid level, a third portion extending downward from a top end of the second portion till the liquid level, and a fourth portion extending upward from a bottom end of the third portion till out of the liquid level and above the biogas residue collecting bin. A pressure-feed flange is disposed at an outer side of the fermentation vessel, a feed channel is formed between the pressure-feed flange and the first portion, and a discharge channel is formed between the pressure-feed flange and the fourth portion.

Abstract: A method of preparing an oxidized starch haemostatic material, including adding a starch raw material to an external circulation pulsatile reactor; switching on a pump to allow inflow of dry air for drying the raw material; switching on a heat exchanger; vacuumizing the reactor; opening a nitrogen dioxide pipeline and a dry air pipeline; pumping in a mixed gas; controlling temperature at 0-120° C. by the heat exchanger; wherein nitrogen dioxide is contacted with the raw material to trigger a selective oxidation reaction and is continuously recycled; an airflow circulation switch is switched on and off at a pulsating ratio of 1:1-10 to carry out airflow pulsation; stopping inflow of nitrogen dioxide and continuing airflow circulation for 5-30 minutes; after completion of the reaction, adjusting to room temperature; vacuumizing the reactor and treating exhaust gas; allowing nitrogen gas to flow in for rinsing the replaced reactor until completion.