Abstract: Embodiments provide methods to produce an assembly that mimics in vitro the human microenvironment of a primary tumor site, a secondary distant organ, and circulation. Methods and apparatuses that provide a platform for the isolation of primary tumor, invasive, and metastatic cells to be used for evaluation of therapeutic efficacy and diagnostics as applied to metastatic cancers are also provided.
Abstract: Embodiments provide methods to produce an assembly that mimics in vitro the human microenvironment of a primary tumor site, a secondary distant organ, and circulation. Methods and apparatuses that provide a platform for the isolation of primary tumor, invasive, and metastatic cells to be used for evaluation of therapeutic efficacy and diagnostics as applied to metastatic cancers are also provided.
Abstract: Embodiments provide methods of preparing a platform, which entails, among others, contacting a) a culture comprising a medium or growth matrix comprising an immune cell, with b) a biological matrix mimetic comprising a target cell such a cancer cell, and allowing, under suitable conditions, the immune cell to migrate to the biological matrix mimetic.