Abstract: The present disclosure describes the engineering of microbial cells for fermentative production of histamine and provides novel engineered microbial cells and cultures, as well as related histamine production methods.

Abstract: The present disclosure describes the engineering of microbial cells for fermentative production of 4-APEA and related products and provides novel engineered microbial cells and cultures, as well as related 4-APEA production methods.

Abstract: The disclosure discusses chiral dopants for liquid-crystalline materials. Chiral dopants can be bioreachable compounds, i.e. compounds produced from microbes through fermentation. Chiral dopants can also include bioreachable materials that are further modified by chemical synthetic steps. Chiral dopants as discussed herein can include biomolecules such as glycyrrhetinic acid (1), S-naringenin (2), shikimic acid (3), alpha-phellandrene (4), betulin (5), malic acid (6), valencene (7), or nootkatone (8), and any stereoisomers or chemically modified derivatives thereof. The disclosure further shows optical properties of such compounds in a liquid-crystalline material.

Abstract: The present invention relates to an optical film comprising a polyimide-based resin having a constitutional unit derived from an aliphatic diamine, wherein the optical film has a glass transition temperature of 165° C. or higher and an optical transmittance at 350 nm of 10% or less.

Abstract: The present disclosure describes the engineering of microbial cells for fermentative production of histamine and provides novel engineered microbial cells and cultures, as well as related histamine production methods.

Abstract: The present invention relates to a method for producing an optical film, the method comprising: step (I) for dissolving a polyimide-based resin in a solvent to prepare a varnish; step (II) for applying the varnish onto a substrate to form a coating film; and step (III) for drying the coating film to form a film, wherein the polyimide-based resin contains a constitutional unit derived from an aliphatic diamine, the solvent in step (I) has a moisture absorption speed per unit area of 25% by mass/h m2 or more as measured by a Karl Fischer method, and a time T from the completion of the formation of the coating film in step (II) to the start of the drying of the coating film in step (III) satisfies the following equation (A): T < 0.0018 Vs ( A ) wherein Vs represents a moisture absorption speed per minute (% by mass/min) of the solvent as determined by a Karl Fischer method.

Abstract: The present invention relates to a method for producing a long optical film, the method comprising a step for preparing a varnish by dissolving a polyimide-based resin in a solvent, wherein the polyimide-based resin contains a constitutional unit derived from an aliphatic diamine, and a moisture absorption speed per unit area of the solvent is 25% by mass/h·m2 or less as determined by a Karl Fischer method.

Abstract: The present disclosure relates to compositions derived from bioreachable molecules, such as amino acids or hydroxy acids. In particular, the composition can be a monomer, a polymer, or a copolymer derived from an amino acid dimer or a hydroxy acid dimer.

Abstract: The present invention relates to an optical film having a glass transition temperature of 165° C. or higher, an optical transmittance at 350 nm of 10% or less, an in-plane retardation of 30 nm or less, and a retardation in a thickness direction of 100 nm or less.

Abstract: A process for the production of dihydronepetalactone including hydrogenating nepetalactone in the presence of a catalytic metal is provided. The catalytic metal may include nickel. The process may be performed in an aqueous medium.

Abstract: A microorganism engineering project employs a vector representation of a biological network. The vector representation allows researchers to input elements known to have a direct impact on a goal of the project (e.g., an element in the metabolic pathway for generating a compound of interest). Within the vector representation, other biological elements are positioned with respect to an input element based on biological relatedness. In this manner, based on positions within the vector representation, candidate elements may be identified for editing. For example, if the elements are associated with a gene, the gene sequence may be knocked out and/or a different promoter may be used for regulating the gene.

Abstract: The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/or Streptomyces Antibiotic Regulatory Protein (SARP) transcription factor.

Abstract: Systems, methods, and non-transitory computer-readable media are described for identifying candidate biological sequences for screening to determine whether the candidate biological sequences enable a biological function. Identification may be based upon (a) degrees of similarity between test sequences and reference sequences that are known to enable the function, and (b) comparisons of regions in the test sequences to reference regions that are known to bind to a molecule. Identification may also be based upon determining sequence similarities between reference sequences, grouping the reference sequences into clusters that each correspond to a molecule that is indicated as capable of being bound by a reference sequence in the cluster, and identifying matching test sequences based upon a comparison of the test sequences with reference sequences in the clusters.

Abstract: High-throughput production of modified microbes is achieved through optimization of directed build graph data structures representing biological workflows. Portions of otherwise unrelated workflows may be combined where they share common biological reaction steps, and processed by a genetic manufacturing facility to take advantage of operational efficiencies. Workflows may be mapped to physical laboratory equipment in a manner that optimizes material transfers. Different automated platforms running different machines in different languages are coordinated in a device-agnostic and language-agnostic manner.

Abstract: Systems, methods and computer-readable media are provided for identifying organisms for production. The identification is based upon determining one or more outlier detection parameters for identifying outliers (e.g., outlier wells, strains, plates holding organisms) from a data set of organism performance metrics. A prediction engine may identify one or more candidate outliers based upon a first set of outlier detection parameters (e.g., outlier detection threshold), and determine probability metrics that represent likelihoods that candidate outliers belong to an outlier class. Based on those metrics, some of the outliers may be excluded from consideration in predicting organism performance for the purpose of selecting organisms for production.

Abstract: The present disclosure provides novel bacterial strains with altered expression or start codon modification of one or more RNA degradation/processing genes. The RNA degradation genes of the present disclosure are controlled by heterologous promoters. The present disclosure further describes methods for generating microbial strains comprising heterologous promoter sequences operably linked to RNA degradation/processing genes.

Abstract: The disclosure relates to host cells having altered NADPH availability, allowing for increased production of compounds produced using NADPH, and methods of use thereof. NADPH availability is altered by one or more of: expressing an altered GAPDH, expressing a variant glutamate dehydrogenase (gdh), aspartate semialdehyde dehydrogenase (asd), dihydropicolinate reductase (dapB), and meso-diaminopimelate dehydrogenase (ddh), expressing a novel nicotinamide nucleotide transhydrogenase, expressing a novel threonine aldolase, and expressing or modulating the expression of a pyruvate carboxylase in the host cells.

Abstract: The present disclosure provides methods and systems for identifying natural product-encoding multi-gene clusters (MGCs). In some embodiments, the present disclosure also teaches methods for producing sequenced and assembled metagenomic libraries that are amenable to MGC search bioinformatic tools and techniques.

Abstract: Embodiments disclosed include systems and methods to generate and/or improve non-sporulating filamentous fungal cells using high-throughput techniques. Embodiments disclosed also include systems and methods for evaluation and iterative adjustment of methods to generate and/or isolate and select desired strains of non-sporulating filamentous fungal cells using high-throughput techniques.

Abstract: Systems, methods and computer-readable media are provided for designing experiments for organisms at a first scale to generate first-scale performance data used in predicting performance of the organisms at a second, larger scale. The design includes determining first-scale screening conditions based at least in part upon the contribution of second-scale conditions to performance parameters of an organism at the second scale. The first-scale screening conditions include one or more proxies for second-scale conditions that cannot be replicated at first scale. The design determines first-scale screening parameters based at least in part upon computer modeling of the metabolism of the organism at the second scale.