Patents Examined by Alexander D. Kim
-
Patent number: 9951363Abstract: A significant fraction of short fibers (fines) is produced while recycling Old Corrugated Containerboards (OCC), which are usually rejected as solid waste stream, requiring landfilling and posing environmental problems. The major component of these fines rejects are primarily cellulose that can be hydrolyzed into sugars for possible fermentation into biofuels, bioplastics or other sugar based products. Use of fines also offers benefits such as negative costs and production of fermentable sugars without requiring complex pretreatment processes, now required to hydrolyze and eliminate inhibitors from hydrolyzate. Enzymatic hydrolysis of reject fines from a recycled OCC mill, employing different strains of cellulases, were investigated. Fillers (up to 30 mass %) in the fines increases the required dosage of enzymes and costs. Enzyme loading can be lowered by addition of surfactants to reduce their inhibitory activity. The nonionic surfactant Triton X-80 improved hydrolysis yields by up to 10 percent points.Type: GrantFiled: March 13, 2015Date of Patent: April 24, 2018Assignee: The Research Foundation for the State University of New York College of Environmental Science and ForestryInventors: Byeong Cheol Min, Bhavin V. Bhayani, Bandaru V. Ramarao
-
Patent number: 9897617Abstract: The present invention relates to a method for detecting at least one direct factor Xa inhibitor in a sample other than citrate plasma, comprising the step of mixing a sample containing a factor Xa inhibitor with a composition containing factor Xa under conditions which allow the factor Xa to release a detectable substance from a chromogenic substrate.Type: GrantFiled: August 11, 2015Date of Patent: February 20, 2018Assignee: Doasense GmbHInventors: Job Harenberg, Roland Krämer
-
Patent number: 9890406Abstract: A method for producing cathine ((1S,2S)-norpseudoephedrine), in which, in a first reaction step, benzaldehyde is reacted with an acetyl donor according to formula (1), where R?H or COOH, by way of an (S)-selective lease to yield an enantiomer mixture according to formulas (2) and (3) and, in a second step, the compound according to formula (3) is reacted with an amine donor by way of an (S)-selective transaminase to yield (1S,2S)-norpseudoephedrine.Type: GrantFiled: May 17, 2014Date of Patent: February 13, 2018Assignee: Forschungszentrum Juelich GmbHInventors: Doerte Rother, Martina Pohl, Torsten Sehl, Alvaro Gomez Baraibar
-
Patent number: 9890374Abstract: The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide.Type: GrantFiled: July 21, 2016Date of Patent: February 13, 2018Assignee: DANISCO US INCInventors: Jing Ge, Ling Hua, Ayrookaran J. Poulose
-
Patent number: 9885025Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: GrantFiled: July 14, 2016Date of Patent: February 6, 2018Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
-
Patent number: 9879249Abstract: The disclosure provides aldehyde-tagged protein carriers that can be covalently and site-specifically bound to drug to provide a drug-containing scaffold. The invention also encompasses methods of production of such drug-containing scaffolds and intermediates, as well as methods of use.Type: GrantFiled: December 4, 2015Date of Patent: January 30, 2018Assignee: Redwood Bioscience, Inc.Inventors: David Rabuka, Mark Alan Breidenbach
-
Patent number: 9868971Abstract: Disclosed are methods and kits for controlling the length of overhangs at the ends of double stranded DNA based on a competitive inhibition. Further provided are ligase-independent methods for joining the two ends of DNA strands using the same. The present methods efficiently control the overhangs of ds DNA. This user-controlled overhangs supply a tool for efficient ligation in a ligase independent way and can be advantageously used in DNA recombinant technology such as cloning gene or DNA fragments of interest or PCR products.Type: GrantFiled: October 28, 2013Date of Patent: January 16, 2018Assignee: ENZYNOMICS CO. LTD.Inventors: Yong Keol Shin, Yunil Suh, Min Young Lee, Ah Rong Jeong
-
Patent number: 9862978Abstract: The present invention relates to a method for easily preparing (2RS)-amino-(3S)-hydroxy-butyric acid, which is a chiral amino acid, in a high yield and a high purity using a chemical synthesis and enzymatic reduction reaction; an intermediate therefor; and a method for preparing the intermediate.Type: GrantFiled: February 24, 2014Date of Patent: January 9, 2018Assignee: Aminologics Co. Ltd.Inventors: Min Seok Kang, Hyun II Lee, Ki Nam Uhm, Jin Hyang Kim
-
Patent number: 9862974Abstract: A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyanoI, is disclosed. The enhanced Cyanobacterium sp. ABICyanoI produces a compound or compounds of interest.Type: GrantFiled: December 23, 2013Date of Patent: January 9, 2018Assignee: Algenol Biotech LLCInventors: Kui Wang, Tuo Shi, Irina Piven, Masami Inaba, Frank Uliczka, Dan Kramer, Heike Enke, Kerstin Baier, Alexandra Friedrich, Ulf Duehring
-
Patent number: 9850192Abstract: Processes and methods for making biobased acrylic acid products including acrylic acid, acrylic acid oligomers, acrylic acid esters, acrylic acid polymers and articles from renewable carbon resources are described herein.Type: GrantFiled: June 7, 2013Date of Patent: December 26, 2017Assignee: CJ Cheiljedang CorporationInventors: Stephen Harris, Kevin A. Sparks, Oliver P. Peoples, Yossef Shabtai, Christopher W. J. McChalicher, Johan van Walsem, Christopher Mirley, Dirk Schweitzer
-
Patent number: 9845475Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: GrantFiled: October 12, 2015Date of Patent: December 19, 2017Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
-
Patent number: 9834758Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.Type: GrantFiled: June 30, 2015Date of Patent: December 5, 2017Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Steven J. Collier, Hans-Georg Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
-
Patent number: 9810701Abstract: The present invention relates to a method for detecting at least one direct factor Xa inhibitor in a sample other than citrate plasma, comprising the step of mixing a sample containing a factor Xa inhibitor with a composition containing factor Xa under conditions which allow the factor Xa to release a detectable substance from a chromogenic substrate.Type: GrantFiled: August 11, 2015Date of Patent: November 7, 2017Assignee: Doasense GmbHInventors: Job Harenberg, Roland Krämer
-
Patent number: 8415457Abstract: The invention provides vitamin K-dependent polypeptides with enhanced membrane binding affinity. These polypeptides can be used to modulate clot formation in mammals. Methods of modulating clot formation in mammals are also described.Type: GrantFiled: October 30, 2007Date of Patent: April 9, 2013Assignee: Regents of the University of MinnesotaInventor: Gary L. Nelsestuen
-
Patent number: 8030024Abstract: The present invention is directed to a method of producing spider dragline and/or flagelliform proteins. The invention is further directed to a method of producing spider threads and to a dragline/flagelliform protein or dragline/flagelliform protein thread produced by these methods. The invention further provides the use of these proteins/threads in the field of biotechnology and/or medicine, in particular in the manufacture of wound closure or coverage systems, suture materials and in the manufacture of replacement materials, preferably artificial cartilage or tendon materials.Type: GrantFiled: December 21, 2006Date of Patent: October 4, 2011Assignee: Amsilk GmbHInventors: Thomas Scheibel, Daniel Huemmerich, Uri Gat
-
Patent number: 7985783Abstract: The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.Type: GrantFiled: September 18, 2007Date of Patent: July 26, 2011Assignee: The Regents of the University of CaliforniaInventors: Isaac S. Carrico, Brian L. Carlson, Peng Wu, Carolyn Bertozzi
-
Patent number: 7973149Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.Type: GrantFiled: July 12, 2007Date of Patent: July 5, 2011Assignee: Martek Biosciences CorporationInventors: James G. Metz, James H. Flatt, Jerry M. Kuner, William R. Barclay
-
Patent number: 7964709Abstract: A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.Type: GrantFiled: September 14, 2007Date of Patent: June 21, 2011Assignee: Ajinomoto Co., Inc.Inventors: Hidehiko Kumagai, Hideyuki Suzuki, Takane Katayama, Miyoko Nawata, Hidetsugu Nakazawa
-
Patent number: 7960524Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.Type: GrantFiled: March 22, 2007Date of Patent: June 14, 2011Assignee: Martek Biosciences CorporationInventors: James G. Metz, James H. Flatt, Jerry M. Kuner, William R. Barclay
-
Patent number: 7947471Abstract: The invention provides a method for production of polypeptides in eukaryote microcarrier cell culture, the method comprising the steps of (i) Culturing cells expressing said polypeptide on microcarriers under conditions and at a setpoint temperature appropriate for expression of said polypeptide; (ii) Cooling the culture to a predetermined temperature below said setpoint; (iii) Sedimenting the microcarriers; and (iv) Harvesting all or part of the culture medium.Type: GrantFiled: September 7, 2007Date of Patent: May 24, 2011Assignee: Novo Nordisk Health Care A/GInventor: Ida Mølgaard Knudsen