Patents Examined by Alexander Kim
  • Patent number: 9856461
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an ?-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the ?-olefin.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: January 2, 2018
    Assignee: The Regents of the University of California
    Inventors: Jeffrey L. Fortman, Leonard Katz, Eric J. Steen, Jay D Keasling
  • Patent number: 9850512
    Abstract: A method for processing a stream of cellulosic fines containing inorganic particles, to increase a hydrolysis yield of polysaccharide degradation enzymes, such fines in a waste stream from a recycled packaging paper mill to produce a stream of fermentable sugars, comprising treating the fines with a surfactant which selectively binds to the inorganic particles and which reduces binding to the inorganic particles by the polysaccharide degradation enzymes, and degrading polysaccharides in the waste stream with the polysaccharide degradation enzymes.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: December 26, 2017
    Assignee: The Research Foundation For The State University of New York
    Inventors: Byeong Cheol Min, Bhavin V. Bhayani, Bandaru V. Ramarao
  • Patent number: 9840699
    Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: December 12, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: David R. Liu, Alexis Christine Komor
  • Patent number: 9834801
    Abstract: Provided are methods for synthesizing compounds, including chiral kynurenine compounds. The methods are suitable for large-scale manufacture and produce the chiral kynurenines compounds in high chemical purity and high chiral purity.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: December 5, 2017
    Inventors: Stefan Abele, Klaus Laue, Roland A. Breitenmoser
  • Patent number: 9828588
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: November 28, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Darrel W. Stafford, Tao Li
  • Patent number: 9823245
    Abstract: The present invention describes the novel molecular entities, nanodisc clathrates, the method of preparation, and the use of these molecular entities for solution phase analysis or crystallization.
    Type: Grant
    Filed: December 20, 2014
    Date of Patent: November 21, 2017
    Inventor: Holly Soutter
  • Patent number: 9803221
    Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: October 31, 2017
    Assignees: Enchi Corporation, Dartmouth College
    Inventors: Yu Deng, Daniel G. Olson, Johannes Pieter van Dijken, Arthur J. Shaw, IV, Aaron Argyros, Trisha Barrett, Nicky Caiazza, Christopher D. Herring, Stephen R. Rogers, Frank Agbogbo
  • Patent number: 9803220
    Abstract: The application describes a method for producing alkenes (for example propylene, ethylene, 1-butylene, isobutylene, isoamylene, butadiene or isoprene) from 3-hydroxy-1-carboxylic acids via 3-sulfonyloxy-1-carboxylic acids.
    Type: Grant
    Filed: October 24, 2013
    Date of Patent: October 31, 2017
    Assignees: Global Bioenergies, Scientist of Fortune, S.A.
    Inventors: Philippe Marliere, Maria Anissimova, Mathieu Allard
  • Patent number: 9803181
    Abstract: Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a Bacillus amylase. In the hybrid, an N-terminal portion of a Termamyl-like ?-amylase is replaced with sequences from an archae ?-amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like ?-amylase with a B domain sequence of an archae ?-amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: October 31, 2017
    Assignee: Danisco US Inc.
    Inventors: Scott D. Power, Andrew Shaw
  • Patent number: 9796966
    Abstract: Provided are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of primer extension methods. Also provided are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: October 24, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith Bauer, Thomas W. Myers, Shawn Suko
  • Patent number: 9783838
    Abstract: The present invention provides novel methods for preparing glycosylated molecules such as oligosaccharides, glycolipids, and glycoproteins/peptides. Novel sialyltransferases are also disclosed. The method includes forming a reaction mixture containing an acceptor molecule, a donor substrate having a sugar moiety and a nucleotide, and a sialyltransferase selected from PmST3 (SEQ ID NO:7) and certain variants thereof. The reaction mixture is formed under conditions sufficient to transfer the sugar moiety from the donor substrate to the acceptor molecule, thereby forming the glycosylated molecule. In some embodiments, the acceptor molecule is selected from a natural product, an oligosaccharide, a glycoprotein, and a glycolipid. In some embodiments, the donor substrate is formed via conversion of a suitable hexosamine derivative to a cytidine 5?-monophosphate(CMP)-sialic acid in a one-pot reaction mixture containing asialic acid aldolase and a CMP-sialic acid synthetase.
    Type: Grant
    Filed: November 7, 2012
    Date of Patent: October 10, 2017
    Assignee: The Regents of the University of California
    Inventors: Xi Chen, Vireak Thon, Hai Yu
  • Patent number: 9777299
    Abstract: The present invention is intended to prove a technique useful for controlling the reaction of oxidoreductase, and to provide a reaction system allowing efficient conversion from carbon dioxide to formic acid, and an efficient methanol production system including the reaction system. The reverse redox reaction is selectively promoted by carrying out the reaction catalyzed by an oxidoreductase using an artificial electron carrier. The reaction system is used for the production of methanol.
    Type: Grant
    Filed: June 13, 2013
    Date of Patent: October 3, 2017
    Assignee: AMANO ENZYME INC.
    Inventors: Yutaka Amao, Shusaku Ikeyama, Yuji Nakanishi, Tetsuya Takahashi, Satoshi Koikeda
  • Patent number: 9777301
    Abstract: Disclosed are methods for producing optically active amino acids and amines. According to the methods, ?-keto acids are generated as reaction intermediates, and as a result, ?-transaminase-catalyzed kinetic resolution of racemic amino acids or amines as racemic amine compounds enables the production of optically active amine compounds without the need to use expensive ?-keto acids as starting materials. Therefore, the optically active amine compounds are produced at greatly reduced costs. In addition, the optically active amine compounds have high enantiomeric excess.
    Type: Grant
    Filed: December 13, 2013
    Date of Patent: October 3, 2017
    Inventors: Jong-Shik Shin, Eul-Soo Park, M. Shaheer Malik
  • Patent number: 9777281
    Abstract: Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: October 3, 2017
    Assignee: Sangamo Therapeutics, Inc.
    Inventor: Edward J. Rebar
  • Patent number: 9777296
    Abstract: Provided is a method for producing bioproducts, including the steps of: culturing a first microorganism to produce bioalcohol; hydrolyzing the first microorganism; separating the bioalcohol; obtaining wastes from the hydrolyzed bioalcohol fermentation; and inoculating a second microorganism into the wastes from the hydrolyzed bioalcohol fermentation.
    Type: Grant
    Filed: May 14, 2013
    Date of Patent: October 3, 2017
    Inventors: Hyo-Hak Song, Julia Lee, Gi-Wook Choi, Se-Kwon Moon
  • Patent number: 9765344
    Abstract: The present invention concerns a method for enhancing the growth rate, the biomass production and/or the fermentative potential of an anaerobic microorganism, comprising the steps consisting in: (a) modifying said anaerobic microorganism by inactivating the ohrR gene, the ohrR gene product or homolog thereof; (b) culturing the modified anaerobic microorganism obtained in step (a) under low-ORP anaerobic conditions.
    Type: Grant
    Filed: June 5, 2012
    Date of Patent: September 19, 2017
    Assignee: Commissariat à l'énergie atomique et aux énergies alternatives
    Inventors: Jean Armengaud, Geremy Clair, Catherine Duport
  • Patent number: 9765362
    Abstract: Processes, systems, and methods for producing combustible gas from wet biomass are provided. In one aspect, for example, a process for generating a combustible gas from a wet biomass in a closed system is provided. Such a process may include growing a wet biomass in a growth chamber, moving at least a portion of the wet biomass to a reactor, heating the portion of the wet biomass under high pressure in the reactor to gasify the wet biomass into a total gas component, separating the gasified component into a liquid component, a non-combustible gas component, and a combustible gas component, and introducing the liquid component and non-combustible gas component containing carbon dioxide into the growth chamber to stimulate new wet biomass growth.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: September 19, 2017
    Assignee: Genifuel Corporation
    Inventor: James R. Oyler
  • Patent number: 9765370
    Abstract: A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: September 19, 2017
    Assignee: Evonik Degussa GmbH
    Inventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
  • Patent number: 9758773
    Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.
    Type: Grant
    Filed: December 12, 2014
    Date of Patent: September 12, 2017
    Assignee: Agilent Technologies, Inc.
    Inventors: Nancy Mckinney, Holly H. Hogrefe, Jeffrey Fox, Connie J. Hansen, Bahram Arezi
  • Patent number: 9758787
    Abstract: The present disclosure provides, inter alia, Thraustochytrium and relevant methods and reagents, including engineered regulatory sequences from and/or operative in Thraustochytrid or Thraustochytrium, selectable markers useful for engineering microorganisms such as Thraustochytrids, means for mutagenizing microorganisms, novel strains produced by mutagenesis, and methods and compositions related to production of particular compounds in microorganisms.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: September 12, 2017
    Inventors: ShuoCheng Zhang, Roberto E. Armenta