Abstract: Novel photolabile photochemical reagents are disclosed. The reagents are useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites into oligonucleotide or polynucleotide chains, i.e., sites which are cleavable upon photolysis. The reagents are also useful in both 5'- and 3'-phosphorylation of oligonucleotide or polynucleotide chains.

Abstract: The invention provides a rapid method for lysing Mycobacteria. In one embodiment is provided a method for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of the enzyme achromopeptidase.The method of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.

Abstract: This invention relates to T7-type DNA polymerases and methods for using them.

Abstract: An aqueous, foam-retarding, printing ink composition consists essentially of:(A) an aqueous thermoplastic or thermosetting printing ink;(B) benzotriazole, fumaric acid, malic acid, hydroquinone, dodecanethiol, succinic anhydride, or adipic acid as foaming or blowing agent modifiers or inhibitors;(C) optionally a C.sub.1 to C.sub.6 water soluble or water dispersible alcohol; and(D) water.The printing ink composition has a pH of about 3 to about 5. The composition is useful for chemically embossing heat expandable or heat foamable resinous materials. The embossed materials are useful, for example, as floor coverings.

Abstract: A method for determining the amino acid sequence of a polypeptide complementary to at least a portion of an original peptide or protein. In one aspect the method involves: (a) determining a first nucleotide sequence of a first nucleic acid coding for the biosynthesis of at least a portion of the original peptide or protein; (b) ascertaining a second nucleotide sequence of a second nucleic acid which base-pairs with the first nucleotide sequence of the first nucleic acid, the first and second nucleic acids pairing in antiparallel directions; and (c) determining the amino acid sequence of the complementary polypeptide by the second nucleotide sequence when read in the same reading frame as the first nucleotide sequence.

Abstract: A skin preparation such as skin lotion or cream which is resistant to being removed when the skin is washed includes a skin-preparation base and a plurality of wash-off-resistance substantivity agents. The wash-off-resistance substantivity agents include a polymeric skin-feel-modifying compound, a quanternary ammonium compound, a hydrophobic modified starch, a substantive silicone and a substantive emollient.

Abstract: The invention relates to a kit for detecting the presence of a nucleic acid sequence, such as a gene or a gene fragment, in a composition or a specimen supposed to contain it. The kit comprises a probe containing a nucleic acid complementary with the nucleic acid sequence or gene which is sought. The probe bears at least one 7-iodo-N-2-acetylamino-fluorene group covalently fixed at one at least of the bases of this probe.

Abstract: The present invention relates to systems and methods used to assay for particular complement component fragments. The invention can be used to determine the amount of a particular complement component fragment in a sample. The fragment can be fluid phase or bound to an immune complex. Generally, specific binding agents, such as antibodies, directed to the complement component fragments and immune complexes are used in the assay.

Abstract: This invention relates to DNA having at least 60% homology relative to .gamma.-RNA or a portion thereof and the capacity of selective hybridization with .delta.-RNA. Such DNA, if provided with a detectable label, such as .sup.32 P can be used as a diagnostic agent to diagnose .delta. infection.

Abstract: The method of this invention is applicable to rapid separation, isolation, and purification of DNA or RNA from biological samples. The DNA/RNA may be in double-stranded or single-stranded form. The method is particularly advantageous for resolving genetic DNA or RNA found in bacteria, virus, and mammalian cells, and for use with samples of human bodily fluids and tissues, including stool, sputum, urine, and blood samples. DNA or RNA can be separated effectively from interfering components, particularly proteins, biological pigments and mucopolysaccharides. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution.

Abstract: Water soluble coloring compositions are provided that contain a stable and uniform suspension of color and sparkle or glitter components. The process for producing the coloring compositions employs a lower temperature range than the prior art; this reduces the need for equipment heating and enables the equipment to be more readily cleaned.

Abstract: Water soluble coloring compositions are provided that contain a stable and uniform suspension of color and sparkle or glitter components. The process for producing the coloring compositions employs a lower temperature range than the prior art; this reduces the need for equipment heating and enables the equipment to be more readily cleaned.

Abstract: Water soluble coloring compositions are provided that contain a stable and uniform suspension of color and sparkle or glitter components. The process for producing the coloring compositions employs a lower temperature range than the prior art; this reduces the need for equipment heating and enables the equipment to be more readily cleaned.

Abstract: A dye used in ink compositions for ink-jet printing comprises an aromatic dye molecule having attached to the backbone thereof from one to four polyhydroxyl-substituted groups having the formula selected from the group consisting of:(a) CH.sub.2 OH-(CHOH).sub.n CH.sub.2 O-, where n=2 to 10;(b) O.sub.n-1 C.sub.n H.sub.2n-1 -O-, where n=5 to 7; and(c) C.sub.6n H.sub.5n+1 O.sub.5n -O-, where n=1 to about 100. These dyes have improved water solubility and improved interaction with paper.

Abstract: The ink compositions of this invention comprise four aqueous component ink compositions including four different water-soluble dyes, namely, water-soluble cyan, magenta, yellow and black dyes. These component ink compositions, when used together, produce a hard copy image having a color gamut that visually more closely corresponds to the color gamut of an image displayed on the color video screen than prior art ink compositions. This specific color gamut is represented by the hereinafter described specific ranges of hue angles in a CIELAB standard chromaticity diagram for each of such component ink compositions. The nature and identity of the component ink compositions are also further described by providing the preferred ranges for each component of the wavelength of maximum absorbance (Lambda max, measured in nm) and for the spectral strength in ml/absorbance unit at that maximum point of absorbance.

Abstract: Ink compositions consisting of small unilamellar or multilamellar vesicles formed from surfactants of anionic, cationic, zwitterionic and nonionic molecules having an oil soluble dye, inclusive of a lipid-soluble dye associated therewith are described. The dye to surfactant ratio is preferably from about 1:1 to about 1:10. The compositions are useful in traditional printing techniques such as flexography and rotogravure and in electronic printing systems such as with an ink-jet printer.

Abstract: Pigments are disclosed which consist essentially of rutile TiO.sub.2 particles bearing coatings comprising SiO.sub.2 and B.sub.2 O.sub.3.

Abstract: A parting composition useful for continuously casting aluminum and aluminum alloys and comprising glycerol trioleate and a vegetable oil. Mixtures of glycerol trioleate with castor oil have superior properties compared with parting compositions previously used for continuous ingot casting.

Abstract: An erasable ball-point pen ink is disclosed. The ink is comprised of a rubber component, a volatile solvent, an organic pigment and a nonvolatile solvent, which additionally contains fine powders of silicon dioxide formed by the hydrolysis of silicon tetrachloride, the hydrolysis being carried out by a high temperature gas phase reaction; a semisolid substance; a soft solid substance having a melting point of not more than 130.degree. C.; a combination of fine powders of silicon dioxide formed by the hydrolysis of silicon tetrachloride, the hydrolysis being carried out by a high temperature gas phase reaction, and a semisolid substance; or a combination of fine powders of silicon dioxide formed by the hydrolysis of silicon tetrachloride, the hydrolysis being carried out by a high temperature gas phase reaction, and a soft solid substance having a melting point not higher than 130.degree. C.