Abstract: Provided are two plant cDNA clones that are homologs of the bacterial CelA genes that encode the catalytic subunit of cellulose synthase, derived from cotton (Gossypium hirsutum).
Type:
Grant
Filed:
October 29, 1997
Date of Patent:
August 7, 2001
Assignee:
Calgene LLC
Inventors:
David M. Stalker, Julie R. Pear, Deborah Delmer
Abstract: Process for the inhibition of the production of a gene product in a plant cell which comprises generating in the cell while the gene product is being expressed mRNA from recombinant DNA coding for part only of the gene product: also constructs for use in the process, and cells and plants that carry out the process. Specifically applicable to control of fruit ripening, in particular in tomatoes.
Type:
Grant
Filed:
December 7, 1993
Date of Patent:
August 7, 2001
Assignee:
Zeneca Limited
Inventors:
Ian G. Bridges, Donald Grierson, Wolfgang W. Schuch
Abstract: The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells.
Type:
Grant
Filed:
August 12, 1996
Date of Patent:
July 31, 2001
Assignees:
Regents of the University of Minnesota, The United States of America
Inventors:
Burle G. Gengenbach, David A. Somers, Donald L. Wyse, John W. Gronwald, Margaret A. Egli, Sheila M. Lutz
Abstract: The present invention provides methods and DNA constructs for the genetic engineering of plant cells to produce plants which produce substantially seedless fruit in the absence of exogenous growth factors (auxins or cytokinins) and in the absence of pollination. The substantially seedless fruits produced by the methods described herein are about the size of wildtype seeded fruit (or somewhat larger) and these fruits are equal to or superior to the wildtype seeded fruit with respect to solid content and flavor. The seedless fruits of the present invention are produced in transgenic plants which contain and express auxin or cytokinin biosynthetic genes, e.g., tryptophan oxygenase or isopentenyl transferase coding sequences expressed under the regulatory control of GH3 or AGL promoter sequences directing preferential or tissue specific expression of a downstream gene in the ovaries or developing fruit.
Type:
Grant
Filed:
May 6, 1998
Date of Patent:
July 31, 2001
Assignee:
Kansas State University Research Foundation
Abstract: cDNAs encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith.
Type:
Grant
Filed:
January 20, 1999
Date of Patent:
July 24, 2001
Assignee:
Washington State University Foundation
Inventors:
Rodney Bruce Croteau, Jörg Bohlmann, Reinhard Jetter, John E. Crock, Christopher L. Steele
Abstract: A method of modifying ethylene biosynthesis in a plant comprises inserting into the genome of the said plant a DNA sequence such as SEQ-ID-NO-1 (encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)) and/or sequence SEQ-ID-NO-2 (encoding an ethylene-forming enzyme (EFE)) which modifies the activity of at least one of ACS or EFE. The method may be used to modify fruit ripening characteristics, especially in bananas.
Type:
Grant
Filed:
January 15, 1999
Date of Patent:
July 17, 2001
Assignee:
Zeneca Limited
Inventors:
Colin Roger Bird, Jonathon David Fletcher
Abstract: Novel plant promoters isolated from cotton fiber tissues are provided. The plant promoters are located upstream cotton plant genes KC03, KC18, KC22, and Gh3, all of which are expressed in cotton fibers, and contain (a) DNA having the nucleotide sequence of SEQ ID NO:1, 6, 11, or 16. Also provided are plant expression vectors prepared by introducing the plant promoters into appropriate vectors; transformed plant cells prepared by introducing the plant expression vectors into host plant cells; transformed plants regenerated from the transformed plant cells; transformed plant seeds obtained from the transformed plants; and processes for producing transformed plants from the plant seeds.
Type:
Grant
Filed:
January 20, 1998
Date of Patent:
July 10, 2001
Assignees:
Toyo Boseki Kabushiki Kaisha, Texas Tech University of Lubbock
Inventors:
Koichi Fujisawa, Yoshihisa Kasukabe, Susumu Nishiguchi, Yoshihiko Maekawa, Randy Dale Allen, Song Ping
Abstract: There are described DNA sequences from Arabidopsis that contain the coding region of amino acid transporters, as well as plasmids, bacteria, yeasts and plants containing these DNA sequences.
Abstract: The present invention relates to the isolation of polyphenol oxidase (PPO) gene fragments from plants. In a first aspect of the present invention there is provided a method for preparing PPO gene fragments, which method includes providing a sample of plant tissue, a first primer in sense orientation having a sequence corresponding to a conserved region of a PPO gene, a second primer in antisense orientation having a sequence corresponding to a conserved region of a PPO gene; isolating genomic DNA from the plant tissue; and amplifying the genomic DNA using the first and second primers. Surprisingly, the applicant has found that PPO genes in plants lack introns and, therefore, fragments of PPO genes may be amplified directly from genomic DNA of a range of plants. The lack of introns means that the size of the fragments can be predicted and bands of the appropriate size can be selected for cloning.
Type:
Grant
Filed:
August 4, 1998
Date of Patent:
June 5, 2001
Assignee:
Commonwealth Scientific and Industrial Research
Organization
Abstract: A method for enhancing the expression of a tomato phytoene synthese gene in a plant while avoiding or reducing co-suppression involves the synthesis of a DNA which is altered in nucleotide sequence (SEQ ID NO:1) and is capable of expression of a protein, ideally identical to that of a protein already expressed by a DNA already present in the plant. This method ensures that sequence similarity between the two genes is reduced enough to eliminate the phenomenon of co-suppression, allowing the over-expression of a specific phytoene synthese protein.
Type:
Grant
Filed:
November 5, 1998
Date of Patent:
May 29, 2001
Assignee:
Zeneca Limited
Inventors:
Caroline Rachel Drake, Colin Roger Bird, Wolfgang Walter Schuch
Abstract: The present invention provides nucleic acid sequences from LEAFY COTYLEDON1 (LEC1) genes encoding LEC1 polypeptides which comprise a CCAAT binding factor domain and which modulate embryo development in plants. The nucleic acids are useful in inducing ectopic development of embryonic tissue or in modulating embryo development in transgenic plants.
Type:
Grant
Filed:
June 24, 1998
Date of Patent:
May 22, 2001
Assignee:
The Regents of the University of California
Inventors:
John J. Harada, Tamar Lotan, Masa-aki Ohto, Robert B. Goldberg, Robert L. Fischer
Abstract: The present invention describes the gene encoding beta-cyclase from marigolds. In particular, the present invention provides the DNA sequence for the gene encoding marigold beta-cyclase as well as vectors containing the same and bacteria and plants transformed with the vectors.
Type:
Grant
Filed:
November 30, 1998
Date of Patent:
May 15, 2001
Assignees:
University of Nevada, University of Maryland
Inventors:
Dean DellaPenna, Francis X. Cunningham, Jr.
Abstract: A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.
Type:
Grant
Filed:
November 19, 1999
Date of Patent:
May 8, 2001
Assignee:
Nara Institute of Science and Technology
Inventors:
Atsuhiko Shinmyo, Kazuya Yoshida, Ko Kato, Koji Akasaka, Takaaki Kusumi, Yoshikazu Tanaka
Abstract: The tomato Cf-5 gene has been cloned and its sequence provided, along with the encoded amino acid sequence. DNA encoding the polypeptide, may be introduced into plant cells and expressed, conferring pathogen resistance on plants comprising such cells and descendants thereof.
Type:
Grant
Filed:
December 16, 1998
Date of Patent:
May 1, 2001
Assignee:
Plant Bioscience Limited
Inventors:
Mark S. Dixon, Kostas Hatzixanthis, David A. Jones, Jonathan D. Jones
Abstract: A novel soybean cultivar, designated 952125710238, is disclosed. The invention relates to the seeds of soybean cultivar 952125710238, to the plants of soybean 952125710238 and to methods for producing a soybean plant produced by crossing the cultivar 952125710238 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 952125710238 with another soybean cultivar.
Abstract: The present invention provides pathogen-resistant transgenic plants and methods of making the plants. The transgenic plants display enhanced resistance to a variety of fungal, bacterial and viral plant pathogens due to expression of a gene that increases the unsaturated fatty acid content of the plant's cells, as compared with an equivalent, but non-transformed plant. The preferred embodiment of the invention is a plant expressing a heterologous &Dgr;-9 desaturase gene from yeast, which particularly increases cytosolic quantities of 16:1, 16:2 and 18:1 fatty acids.
Type:
Grant
Filed:
August 28, 1998
Date of Patent:
May 1, 2001
Assignee:
Rutgers, the State University of New Jersey
Abstract: A novel soybean cultivar, designated 9521427112273, is disclosed. The invention relates to the seeds of soybean cultivar 9521427112273, to the plants of soybean 9521427112273 and to methods for producing a soybean plant produced by crossing the cultivar 9521427112273 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 9521427112273 with another soybean cultivar.
Abstract: DNA encoding glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, plant genes encoding the glyphosate-tolerant enzymes, and plant transformation vectors containing the genes are disclosed. The DNA encodes glyphosate-tolerant EPSP synthases modified by substitution of an alanine residue for a glycine residue in a first conserved sequence found between positions 80 and 120, and a threonine residue for an alanine residue in a second conserved sequence found between positions 170 and 210 in the mature wild type EPSP synthase.
Type:
Grant
Filed:
February 1, 1999
Date of Patent:
May 1, 2001
Assignee:
Monsanto Company
Inventors:
David Alan Eichholtz, Charles Scott Gasser, Ganesh Murthy Kishore
Abstract: The preparation and use of nucleic acid fragments encoding acyl-acyl carrier protein thioesterase enzymes to modify plant lipid composition are disclosed. Also disclosed are chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences may be used to create transgenic plants with altered levels of saturated fatty acids.