Abstract: Methods of genetically manipulating biologically active 4?-O-methylated isoflavonoids have been found based upon the regiospecifity of isoflavone 7-OMT in vivo. Upon transformation and expression of an isoflavonoid O-methyltransferase gene, up-regulation of IOMT in the transgenic plants can be used to increase the accumulation of 4?-O-methylated isoflavonoid phytolalexins, providing for increased disease resistance to the plant. Similar methods can be used to increase accumulation of 4?-O-methylated isoflavonoid nutraceuticals in plants. For down-regulation of IOMT in plants that naturally make 4?-O-isoflavonoid phytoalexins and 4?-O-methylated isoflavonoid nutraceuticals, IOMT gene sequences can be transformed in the antisense orientation.

Abstract: A modified ubiquitin regulatory system which lacks heatshock elements and so is not heat inducible can be used to regulate expression of a structural gene under the control thereof, e.g. by introducing an appropriate DNA construct into plant tissue.

Abstract: Protein Fragment Complementation Assays (PCA) are done in plant material using enzyme fragment constructs, for example, dihydrofolate reductase (DHFR) fragment constructs. Plant material is transformed with at least two different constructs that form different products capable of interacting to reconstitute enzymatic activity in the plant material. Detection of the activity can be done using a substrate for the enzyme in the culture medium which, when reacted with the enzyme, is converted to a detectable product. One embodiment uses a substrate that can be enzymatically converted to a detectable fluorescent product. An inducer such as rapamycin or salicylic acid can be added to the culture medium to increase the level of detectable product.

Abstract: An inbred corn line designated N10018, to the plants of the inbred corn line N10018 and to the methods for producing corn plants by crossing the inbred corn line with itself or another corn line. The invention further relates to methods for producing corn plants containing in its genetic material one or more transgenes from the inbred N10018, and to the transgenic plants produced by that method.

Abstract: The invention concerns a microspore-specific promoter and its use for obtaining plants with gametophytic male sterility with inducible fertility. It also concerns a method for obtaining hybrid plants.

Abstract: The present invention is directed to a composition for altering the levels of seed proteins in cereal grain. The invention is directed to the alteration of seed protein levels in plant grain, resulting in grain with increased digestibility/nutrient availability, improved amino acid composition/nutritional value, increased response to feed processing, improved silage quality, and increased efficiency of wet or dry-milling. The composition provided comprises a nucleotide sequence encoding a maize 18 kD alpha-globulin cDNA as well as expression cassettes comprising nucleotide sequences of the invention. Also provided are transformed plant tissue including plants, cells and seeds thereof.

Abstract: An inbred maize line, designated NP2174, the plants and seeds of inbred maize line NP2174 and descendants thereof, methods for producing a maize plant produced by crossing the inbred line NP2174 with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line NP2174 with another maize line or plant.

Abstract: DNA sequences encoding plant vde enzymes are provided herein. The sequences may be joined to heterologous DNA sequences for use as probes and in DNA constructs to modify the genotype of a host organism. DNA constructs and methods are provided to modify a host cell phenotype by altering the amount of photoprotection enzyme present in the host cell. In plastid containing host cells, zeaxanthin levels and sensitivity to light can be modified through alterations in the level of vde enzymes.

Abstract: The present invention provides and includes nucleic acids, proteins and antibodies associated with novel genes in the MEP pathway. The invention further encompasses methods utilizing such molecules, for example in gene isolation, gene analysis and the production of transgenic plants. The present invention also includes transgenic plants modified to express proteins associated with the MEP pathway.

Abstract: The invention provides isolated auxin-responsive transcriptional regulatory elements and polynucleotides associated therewith. The present invention provides methods and compositions related to altering gene expression in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

Abstract: The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.

Abstract: An inbred corn line designated HX894, to the plants of the inbred corn line HX894 and to the methods for producing corn plants by crossing the inbred corn line with itself or another corn line. The invention further relates to methods for producing corn plants containing in its genetic material one or more transgenes from the inbred HX894, and to the transgenic plants produced by that method.

Abstract: The present invention provides a Populus 4-coumarate Co-enzyme A ligase gene promoter that directs expression in the xylem of plants. The promoter is used in methods designed to alter lignin content, lignin structure, cellulose content and combinations thereof. The methods comprise operably linking said promoter to heterologous nucleic acid molecules.

Abstract: There is provided an aldehyde oxidase gene which is a 4.4 kbp gene obtainable from a plant and which encodes an amino acid sequence of an enzyme capable of oxidizing an aldehyde compound to a carboxylic acid and utilization thereof.

Abstract: The present invention relates to corn plant PV-ZMGT32 (nk603 ) having a transformation event that produces glyphosate tolerance. The invention also relates to progeny corn plants having this event.

Abstract: Disclosed are transgenic plants having edible portions that produce methional during processing. The plants contain increased methionine levels such that upon processing of the edible portion(s), methional levels are increased and lead to food products that possess improved flavor stability and/or quality. Plants of the Solanaceous family e.g., potato, tomato and eggplant, and other methional-producing plants including maize and soybean, are preferred plants. Several ways of genetically engineering plants to produce increased free Met levels are disclosed, with introduction of a non-native nucleic acid encoding cystathionine gamma synthase (CGS) and tissue-specific expression of an anti-sense S-adenosyl-methionine synthetase being preferred. Also disclosed are methods for selecting transformed plant cells using ethionine and CGS as the selection agent and marker gene respectively.

Abstract: The present invention is drawn to a maize lls1 promoter that can be used in DNA constructs to express polynucleotides in plants, plant cells, tissues and seeds.

Abstract: Broadly this invention provides an invention which is inbred corn line G2202. The methods for producing a corn plant by crossing the inbred line G2202 are also encompassed by the invention. Additionally, the invention relates to the various parts of inbred G2202 including culturable cells.

Abstract: A novel potato cultivar of the genus and species Solanum tuberosum, designated FL1909, is disclosed. The invention relates to the tubers of potato variety FL1909, to the plants of potato variety FL1909, to the seeds of the potato variety and to methods for producing a hybrid potato variety. The invention relates to methods of producing potato tubers, seeds and plants by crossing the potato variety FL1909 with another potato plant. The invention further relates to methods of using potato variety FL1909 to produce genetically transformed potato plants.

Abstract: The invention provides isolated polynucleotides encoding MRE11 binding polypeptides, recombinant expression cassettes, host cells, and transgenic plants comprising said polynucleotides.