Patents Examined by Anand U Desai
  • Patent number: 10406156
    Abstract: This application discloses a composition comprising an amiloride and/or an amiloride analog which can be used for reducing nerve injury or nervous system injury in a subject. The formulation of such composition is also disclosed. The application further directs to methods for treating nerve injury or nervous system injury by administering to the subject a therapeutically effective amount of a pharmaceutical composition comprising amiloride, an amiloride analog or a pharmaceutically acceptable salt thereof.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: September 10, 2019
    Assignee: MOREHOUSE SCHOOL OF MEDICINE
    Inventors: Zhigang Xiong, Roger P. Simon
  • Patent number: 10400254
    Abstract: The present invention relates to terpene synthases capable of degrading precursors into biofuel compounds, such as terpenoid compounds. In one instance, a transformed organism can include such terpene synthases, as well as vectors encoding such synthases. Methods of employing such synthases and organisms are also described herein.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: September 3, 2019
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Weihua Wu, John Michael Gladden, Benjamin Chiau-Pin Wu, Ryan Wesley Davis
  • Patent number: 10400019
    Abstract: The present disclosure relates to keratin nanomaterials, methods for obtaining keratin nanomaterials, and biomaterials made from keratin nanomaterials. In particular, keratin nanomaterials comprising Type I and Type II monomer pairs are disclosed as well as a method for obtaining keratin nanomaterials comprising obtaining a solution of keratin and processing the solution by ultrafiltration with buffer solution containing phosphate.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: September 3, 2019
    Assignee: Virginia Tech Intellectual Properties, Inc.
    Inventors: Mark Van Dyke, Maria Rahmany
  • Patent number: 10392649
    Abstract: A polypeptide biosensor that detects free NAD+ is disclosed. The polypeptide comprises a first fragment from an NAD+ dependent DNA ligase acetylation domain, a second fragment from the NAD+ dependent DNA ligase acetylation domain, and a fluorescent protein, wherein the fluorescent protein is positioned between the two DNA ligase acetylation domain fragments. Also disclosed are expression vectors comprising the biosensor as well as methods of using the biosensor to detect NAD+.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: August 27, 2019
    Assignee: Oregon Health & Science University
    Inventors: Richard Goodman, Michael Cohen, Lulu Cambronne, Melissa Stewart
  • Patent number: 10385102
    Abstract: Aspects of the invention relate to the engineering of biological nanostructures and materials.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: August 20, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Timothy Kuan-Ta Lu, Allen Yuyin Chen
  • Patent number: 10385170
    Abstract: The invention relates to a method, a device and a system for producing particularly a hydrogel (200) and for controlling an enzymatically catalyzed formation of a covalent bond in a solution, wherein said covalent bond is formed between a first compound (20) comprising a first moiety (21) and a second compound (22) comprising a second moiety (23), wherein the first and the second moiety (21, 23) are a substrate of an enzyme wherein said enzyme catalyzes the formation of a covalent bond between the first and the second moiety (21, 23), and wherein a voltage is applied to the solution for spatially controlling said formation, wherein said voltage is adjusted such that it induces electrolysis of said solution.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: August 20, 2019
    Assignee: UNIVERSITAT ZURICH
    Inventors: Vincent Milleret, Benjamin R. Simona, Janos Voros, Martin Ehrbar
  • Patent number: 10377812
    Abstract: The present invention describes a simple purification process for recombinant human serum albumin. The process results in highly purified protein with limited number of purification steps. The broth containing human albumin is clarified by centrifugation and microfiltration, diafiltered and captured by cation exchange chromatography by a process that allows 140-230 mg of albumin to be captured per ml of resin. Product related impurities are removed by hydrophobic interaction chromatography, optimized to allow 87-97% recovery in flow through mode. The final series of processes are so combined that there is easy transition from one step to the next with minimal interventions and adjustments. The entire process of purification is completed within two days from harvest to final product. Thus a cost-effective process with improved recovery of protein at each step is developed. The purified human serum albumin is analyzed for purity and shows physicochemical characteristics that are similar to standard albumin.
    Type: Grant
    Filed: January 1, 2016
    Date of Patent: August 13, 2019
    Assignee: NAVYA BIOLOGICALS PVT LTD
    Inventor: Rajyashri Ramakrishna Karur
  • Patent number: 10377998
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends the engineering of optimized modular CRISPR-Cas enzyme systems.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 13, 2019
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology
    Inventors: Feng Zhang, Bernd Zetsche
  • Patent number: 10364419
    Abstract: The present invention relates to a polypeptide binding to human serum albumin and comprising or consisting of an amino acid sequence selected from the group consisting of: (a) GVTLFVALYDY(X1)(X2)(X3)(X4)(X5)(X6)D(X7)SFHKGEKFQIL(X8)(X9)(X10)(X11)(X12)G(X13)(X14)W(X15)(X16)RSLTTG(X17)(X18)G(X19)IPSNYVAPVDSIQ (SEQ ID NO: 1), wherein (X1) is A, V, I, L, M, G, P, S, T, N, Q, C, R, H, K, D or E; (X2) is R, H, K, A, V, I, L, M, G, P, S, T, N, Q or C; (X3) is R, H, K, S, T, N, Q, C, F, Y, W, A, V, I, L, M, G or P; (X4) is S, T, N, Q, C, A, V, I, L, M, G, P, R, H, K, F, Y, W, D or E; (X5) is S, T, N, Q, C, D, E, F, Y, W, A, V, I, L, M, G, P, R, H or K; (X6) is F, Y, W, A, V, I, L, M, G, P, R, H, K, S, T, N, Q or C; (X7) is A, V, I, L, M, G, P, R, H or K; (X8) is S, T, N, Q, C, D or E; (X9) is S, T, N, Q, C, D, E, A, V, I, L, M, G, P, F, Y or W; (X10) is A, V, I, L, M, G or P; (X11) is F, Y, W, R, H or K; (X12) is S, T, N, Q, C, F, Y or W; (X13) is F, Y, W, R, H, K, S, T, N, Q, C, D, E, A, V, I, L, M, G or P; (X14) is
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: July 30, 2019
    Assignee: Covagen AG
    Inventors: Fabian Buller, Ulrich Wüllner, Irene Zbinden, Isabella Attinger-Toller, Ulrike Von Der Bey, Susann König-Friedrich, Julian Bertschinger, Dragan Grabulovski, Patricia Henne
  • Patent number: 10344316
    Abstract: Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: July 9, 2019
    Assignee: Syngenta Participations AG
    Inventors: Tricia C. Moser, Rene Quadt
  • Patent number: 10336796
    Abstract: A system, including methods and compositions, for treatment of ischemia.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: July 2, 2019
    Assignee: MOREHOUSE SCHOOL OF MEDICINE
    Inventors: Roger P. Simon, Zhigang Xiong
  • Patent number: 10314938
    Abstract: The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.
    Type: Grant
    Filed: February 16, 2017
    Date of Patent: June 11, 2019
    Assignee: TRUSTEES OF TUFTS COLLEGE
    Inventors: David L. Kaplan, Ung-Jin Kim, Jaehyung Park, Hyoung-Joon Jin
  • Patent number: 10316058
    Abstract: A method of extracting biomolecules from live cells comprising: introducing a plurality of magnetized carbon nanotubes (MCNTs) into a live cell, wherein the MCNTs penetrate the cell membrane under a magnetic force; spearing the MCNTs through the cell under the magnetic force, wherein a biomolecule attaches to at least a portion of the MCNTs to form a biomolecule loaded MCNT; removing at least a portion of the biomolecule loaded MCNTs from the cell under the magnetic force; and collecting at least a portion of the biomolecule loaded MCNTs.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: June 11, 2019
    Assignee: UNIVERSITY OF HOUSTON SYSTEM
    Inventors: Zhifeng Ren, Dong Cai, Zhen Yang
  • Patent number: 10316313
    Abstract: A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: June 11, 2019
    Assignee: Cornell University
    Inventors: Stephane C. Corgie, Patarawan Kahawong, Emmanuel P. Giannelis, Larry P. Walker
  • Patent number: 10300152
    Abstract: Disclosed are methods, systems, and devices for implementing nanoparticles to encapsulate biomolecules such as enzymes. In one aspect, a nanoparticle device includes a shell structure including an internal layer structured to enclose a hollow interior region and include one or more holes penetrating through the internal layer, and an external layer formed of a porous material around the internal layer; and an enzyme contained within the interior region of the shell structure, the enzyme having entered the shell structure through the one or more holes and incapable of passing through the external layer, in which the pores are of a size that prevents the enzyme to pass through the pores while permitting substances smaller than the pore size to pass through the pores.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: May 28, 2019
    Assignees: The Regents of the University of California, Devacell, Inc.
    Inventors: Inanc Ortac, Sadik C. Esener, Ibrahim Gokce Yayla, Bradley Messmer
  • Patent number: 10287332
    Abstract: The present invention provides amyloid fibrils comprising a plurality of modified ? solenoid protein (mBSP) monomers. The mBSP monomers are modified to enhance self-assembly and are useful in a variety of applications.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: May 14, 2019
    Assignee: The Regents of the University of California
    Inventors: Daniel Cox, Gang-Yu Liu, Michael Toney, Xi Chen, Josh Hihath, Gergely Zimanyi, Natha Robert Hayre, Maria Peralta
  • Patent number: 10285945
    Abstract: The present invention includes compositions and methods for preparing micron-sized or submicron-sized particles by dissolving a water soluble effective ingredient in one or more solvents; spraying or dripping droplets solvent such that the effective ingredient is exposed to a vapor-liquid interface of less than 50, 100, 150, 200, 250, 200, 400 or 500 cm?1 area/volume to, e.g., increase protein stability; and contacting the droplet with a freezing surface that has a temperature differential of at least 30° C. between the droplet and the surface, wherein the surface freezes the droplet into a thin film with a thickness of less than 500 micrometers and a surface area to volume between 25 to 500 cm?1.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: May 14, 2019
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Keith P. Johnston, Joshua Engstrom, Robert O. Williams, III
  • Patent number: 10286004
    Abstract: Targeting-enhanced anticancer nanoparticles and preparation methods for the nanoparticles are provided. In particular, this invention provides targeting-enhanced anticancer nanoparticles comprising non-covalently bound anticancer drugs, serum albumins as the nanoparticle base material, and porphyrin compounds as tumor-targeting moieties. The targeting-enhanced anticancer nanoparticles described in the present invention are characterized by non-covalently bound constituents: anticancer drugs, serum albumin as the nanoparticle base material, and porphyrin compounds as tumor-targeting moieties, thus preventing common toxic side effects, enhancing the structural stability of nanoparticles, and enhancing tumor targeting by treatment with electromagnetic waves due to the activity of porphyrin, without structural and functional changes.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: May 14, 2019
    Assignee: JINIS CO., LTD.
    Inventors: Hyeon Jin Kim, Seong Tshool Hong, Hea Jong Chung, Min Kyu Joo, Hea Guk Cho, Jinny Hong
  • Patent number: 10280426
    Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: May 7, 2019
    Assignee: Novozymes A/S
    Inventors: Carsten Olsen, Michael Dolberg Rasmussen
  • Patent number: 10259851
    Abstract: Methods are provided for extracting DNA-compaction proteins from biological samples.
    Type: Grant
    Filed: May 29, 2014
    Date of Patent: April 16, 2019
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Peter Stanley Gagnon, Hui Theng Gan