Patents Examined by Andrew Wang
  • Patent number: 7713721
    Abstract: This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
    Type: Grant
    Filed: December 1, 2004
    Date of Patent: May 11, 2010
    Assignees: The Scripps Research Institute, The Regents of the University of California
    Inventors: Peter G. Schultz, Lei Wang, John Christopher Anderson, Jason Chin, David R. Liu, Thomas J. Magliery, Eric L. Meggers, Ryan Aaron Mehl, Miro Pastrnak, Stephen William Santoro, Zhiwen Zhang
  • Patent number: 7704949
    Abstract: Methods are described for preventing or reducing ischemia, e.g., cerebral ischemia, and/or reperfusion injury, e.g., reperfusion injury associated with cerebral ischemia, in a patient.
    Type: Grant
    Filed: August 24, 2006
    Date of Patent: April 27, 2010
    Assignee: Dyax Corp.
    Inventors: Robert C. Ladner, Arthur C. Ley, Shirish Hirani, Anthony Williams, Maria Grazia Simoni, Luigi Bergamaschini
  • Patent number: 7700320
    Abstract: Disclosed are a fatty acid synthase (FAS) from Schizochytrium, biologically active fragments and homologues thereof, a nucleic acid sequence encoding such FAS, fragments and homologues thereof, the gene encoding Schizochytrium FAS, host cells and organisms that recombinantly express the FAS, host cells and organisms in which the expression and/or activity of the endogenous FAS has been attenuated, and various methods for making and using any of these proteins, nucleic acid molecules, genes, host cells or organisms.
    Type: Grant
    Filed: February 14, 2005
    Date of Patent: April 20, 2010
    Assignee: Martek Biosciences Corporation
    Inventors: James G. Metz, Craig A. Weaver, Jerry Kuner
  • Patent number: 7700103
    Abstract: Synthetic DNA molecules encoding the HPV 52 L1 protein are provided. Specifically, the present invention provides polynucleotides encoding HPV 52 L1 protein, wherein said polynucleotides are codon-optimized for high level expression in a yeast cell. In alternative embodiments of the invention, the nucleotide sequence of the synthetic molecule is altered to eliminate transcription termination signals that are recognized by yeast. The synthetic molecules may be used to produce HPV 52 virus-like particles (VLPs), and to produce vaccines and pharmaceutical compositions comprising the HPV 52 VLPs. The vaccines of the present invention provide effective immunoprophylaxis against papillomavirus infection through neutralizing antibody and cell-mediated immunity and may also be useful for treatment of existing HPV infections.
    Type: Grant
    Filed: March 18, 2005
    Date of Patent: April 20, 2010
    Assignee: Merck & Co., Inc.
    Inventors: Janine T. Bryan, Michelle K. Brownlow, Loren D. Schultz, Kathrin U. Jansen
  • Patent number: 7691963
    Abstract: Modified exendins and exendin agonists having an exendin or exendin agonist linked to one or more molecular weight increasing compounds, for example, polyamino acids, polyethylene glycol polymers, and related formulations and dosages and methods of administration thereof are provided. These modified exendins and exendin agonists, compositions comprising the modified exendin or the agonist thereof are useful in treating diabetes and conditions that would be benefited by lowering plasma glucose or delaying and/or slowing gastric emptying or inhibiting food intake.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: April 6, 2010
    Assignee: Amylin Pharmaceuticals, Inc.
    Inventors: Kathryn S. Prickett, Andrew A. Young
  • Patent number: 7674458
    Abstract: Described are methods of treating and preventing conditions associated with a loss of elastic fibers. Also provided herein are methods of screening for agents useful in treating such conditions, and animal models of conditions associated with a loss of elastic fibers.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: March 9, 2010
    Assignee: Massachusetts Eye & Ear Infirmary
    Inventors: Tiansen Li, Xiaoqing Liu
  • Patent number: 7674606
    Abstract: A fusion protein containing a polyhedrin of a virus and a foreign polypeptide sequence. Also disclosed are a recombinant occlusion body containing the fusion protein, an isolated nucleic acid containing a sequence encoding the fusion protein, and a recombinant virus containing the nucleic acid. Within the scope of this invention are related expression vectors, host cells, compositions, and preparation methods.
    Type: Grant
    Filed: October 16, 2007
    Date of Patent: March 9, 2010
    Assignee: Academia Sinica
    Inventors: Yu-Chan Chao, Tzu-Ching Lee
  • Patent number: 7670803
    Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
    Type: Grant
    Filed: April 3, 2009
    Date of Patent: March 2, 2010
    Assignee: Meji Seika Kaisha, Ltd.
    Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi
  • Patent number: 7666640
    Abstract: The present invention discloses an expression method for non-naturally-occurring amino acid-containing protein comprising: expressing in animal cells: (A) a mutant tyrosyl-tRNA synthetase that is a mutation of tyrosyl-tRNA synthetase originating in E. coli with an enhanced specificity for a non-naturally-occurring tyrosine derivative as compared with the specificity for tyrosine; (B) suppressor tRNA originating in Bacillus species, Mycoplasma species or Staphylococcus species of eubacteria and capable of binding with the tyrosine derivative in the presence of the mutant tyrosyl tRNA synthetase; and, (C) a desired protein gene that has undergone a nonsense mutation at a desired site; wherein, the tyrosine derivative is incorporated at the location of this nonsense mutation.
    Type: Grant
    Filed: June 19, 2009
    Date of Patent: February 23, 2010
    Assignee: Riken Japan Science and Technology Agency of 1-8
    Inventors: Shigeyuki Yokoyama, Mikako Shirouzu, Ayako Sakamoto, Kensaku Sakamoto
  • Patent number: 7662587
    Abstract: Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production relative to control Escherichia host cells. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
    Type: Grant
    Filed: March 5, 2009
    Date of Patent: February 16, 2010
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Qiong Cheng, Kevin Michael Croker, Kristin Ruebling-Jass, Hong Wang
  • Patent number: 7638300
    Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.
    Type: Grant
    Filed: October 18, 2006
    Date of Patent: December 29, 2009
    Assignees: The Scripps Research Institute, The Regents of the University of California
    Inventors: Peter Schultz, Lei Wang, John Christopher Anderson, Jason W. Chin, David R. Liu, Thomas J. Magliery, Eric Meggers, Ryan Aaron Mehl, Miro Pastrnak
  • Patent number: 7638299
    Abstract: Modified biosynthetic polypeptide molecules, methods for manufacturing, and uses thereof are provided.
    Type: Grant
    Filed: July 21, 2005
    Date of Patent: December 29, 2009
    Assignee: Ambrx, Inc.
    Inventors: Ho Sung Cho, Thomas O. Daniel, Anna-Maria Hays, Troy E. Wilson, David C. Litzinger, Roberto Mariani, Bruce E. Kimmel, William M. Keefe
  • Patent number: 7635579
    Abstract: The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be quantified by the formula: [(g amino acid produced/g dextrose consumed)*100]=% Yield and expressed as yield from dextrose. The invention provides microorganisms that are made auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids by mutagenesis and selected for their ability to produce higher percent yields of the desired amino acid than the parental strain. Preferred microorganisms are Corynebacterium, Brevibacterium or Escherichia coli producing L-lysine. Mutagenesis is performed by classical techniques or through rDNA methodology.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: December 22, 2009
    Assignee: Archer-Daniels-Midland Company
    Inventors: P. John Rayapati, Corey M. Crafton
  • Patent number: 7632823
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: December 1, 2005
    Date of Patent: December 15, 2009
    Assignee: Ambrx, Inc.
    Inventor: Feng Tian
  • Patent number: 7625717
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Grant
    Filed: February 22, 2007
    Date of Patent: December 1, 2009
    Assignee: The Scripps Research Institute
    Inventors: Jason W. Chin, T. Ashton Cropp, J. Christopher Anderson, Peter G. Schultz
  • Patent number: 7618775
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Grant
    Filed: April 16, 2004
    Date of Patent: November 17, 2009
    Assignee: The Scripps Research Institute
    Inventors: Jason W. Chin, T. Ashton Cropp, J. Christopher Anderson, Peter G. Schultz
  • Patent number: 7615360
    Abstract: The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.
    Type: Grant
    Filed: July 10, 2007
    Date of Patent: November 10, 2009
    Assignee: Sanofi-Aventis Deutschland GmbH
    Inventors: Guenter Mueller, Silke Dlugai, Doerthe Voss, Eckhard Boles
  • Patent number: 7611872
    Abstract: The invention relates to an improved method for the production of D-pantothenic acid and/or salts thereof and use thereof as adjunct for animal feedstuffs.
    Type: Grant
    Filed: February 20, 2002
    Date of Patent: November 3, 2009
    Assignee: BASF Aktiengesellschaft
    Inventors: Christine Beck, Hans-Peter Harz, Daniela Klein, Martin Leemann, Markus Lohscheidt, Stefan Bitterlich, Hartwig Vo╬▓
  • Patent number: 7608423
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Grant
    Filed: February 22, 2007
    Date of Patent: October 27, 2009
    Assignee: The Scripps Research Institute
    Inventors: Jason W. Chin, T. Ashton Cropp, J. Christopher Anderson, Peter G. Schultz
  • Patent number: 7608421
    Abstract: The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
    Type: Grant
    Filed: January 22, 2003
    Date of Patent: October 27, 2009
    Assignee: Organobalance GmbH
    Inventors: Christine Lang, Markus Veen