Patents Examined by Ardin Marschel
  • Methods of assessing a risk of cancer progression
    Patent number: 8911940
    Abstract: Methods and kits that use miRNA expression to predict the development of brain metastases in non-small cell lung cancer patients are disclosed.
    Type: Grant
    Filed: July 29, 2010
    Date of Patent: December 16, 2014
    Assignees: The Translational Genomics Research Institute, Scottsdale Healthcare Corporation
    Inventors: Glen Weiss, Seungchan Kim, Sara Nasser
  • Fluid transfer control for real-time PCR
    Patent number: 8906306
    Abstract: The present invention provides a fluid transfer control method, the method based on measurements of intensities of dyes within the fluid to be transferred. In more detail, the present invention makes use of control dyes and quencher molecules for the fluid transfer controls.
    Type: Grant
    Filed: March 29, 2010
    Date of Patent: December 9, 2014
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Gregor Sagner, Martin Horat
  • Biosensors for biological or chemical analysis and systems and methods for same
    Patent number: 8906320
    Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: December 9, 2014
    Assignee: Illumina, Inc.
    Inventors: Helmy A. Eltoukhy, Robert C. Kain, Wenyi Feng, Mark Pratt, Bernard Hirschbein, Poorya Sabounchi
  • Methods for reducing the range in concentrations of analyte species in a sample
    Patent number: 8906608
    Abstract: The present invention relates to the fields of molecular biology, combinatorial chemistry and biochemistry. Particularly, the present invention describes methods and kits for dynamically reducing the variance between analyte taken from complex mixtures.
    Type: Grant
    Filed: July 28, 2006
    Date of Patent: December 9, 2014
    Assignees: Bio-Rad Laboratories, Inc., American National Red Cross
    Inventors: Egisto Boschetti, David Hammond
  • Preparation of glassified biological reagents
    Patent number: 8900525
    Abstract: The invention related to a method of making a dried reagent preparation, comprising the steps of: providing an aqueous solution of at least one buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form a mixture wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition; dispensing the mixture in the form of substantially uniform droplets into wells of a multi-well container, wherein a single droplet is dispensed into each well; drying the droplets in the container to form the reagent preparation. The reagent preparation is water soluble and has a Tg sufficient for room temperature stability.
    Type: Grant
    Filed: September 13, 2007
    Date of Patent: December 2, 2014
    Assignee: GE Healthcare Bio-Sciences Corp.
    Inventors: Reddy Ponaka, Joseph W. Farchaus, Michael D. Pierce
  • Use of biomarkers in methods for the treatment of hepatocellular carcinoma
    Patent number: 8901110
    Abstract: Provided herein are biomarkers for hepatocellular carcinoma and uses thereof.
    Type: Grant
    Filed: January 11, 2013
    Date of Patent: December 2, 2014
    Assignee: Celgene Corporation
    Inventors: Miran Kim, Jack R. Wands, Sarah Beseme
  • Sample preparation, processing and analysis systems
    Patent number: 8894946
    Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
    Type: Grant
    Filed: October 19, 2012
    Date of Patent: November 25, 2014
    Assignee: IntegenX Inc.
    Inventors: William D. Nielsen, Richard J. Belcinski, Gregory Bogdan, David Eberhart, Omar El-Sissi, Stevan B. Jovanovich, Michael Recknor, Ezra Van Gelder, David W. Wyrick
  • Methods and solutions for inhibiting undesired cleaving of labels
    Patent number: 8883999
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: March 17, 2009
    Date of Patent: November 11, 2014
    Assignee: Intelligent Bio Systems, Inc.
    Inventors: Jerzy Olejnik, Evan Guggenheim, Visalakshi Visalakshi
  • Sample preparation devices and systems
    Patent number: 8883088
    Abstract: Devices and system for preparing samples are described. Such devices can comprise fluidic chambers, reservoirs, and movable structures for controlling the movement of samples. The device can also comprise functional elements for performing specific operations.
    Type: Grant
    Filed: February 28, 2012
    Date of Patent: November 11, 2014
    Assignee: California Institute of Technology
    Inventors: Imran R. Malik, Axel Scherer
  • Method for producing and identifying soluble protein domains
    Patent number: 8852866
    Abstract: Methods for producing and identifying fragments of proteins, and more particularly to methods for generating and identifying soluble protein domains are disclosed based on a method for generating a library of nucleic acid fragments from nucleic acid encoding a desired polypeptide, and more especially a library of essentially, randomly sampled fragments of coding DNA sequence predominantly of defined size range and a method for selecting cloned gene fragments from the library that encode soluble protein domains.
    Type: Grant
    Filed: November 8, 2002
    Date of Patent: October 7, 2014
    Assignee: Domainex Limited
    Inventors: Mark McAlister, Renos Savva, Laurence Pearl, Chrisostomos Prodromou, Paul C Driscoll
  • Luminescence detection method
    Patent number: 8852894
    Abstract: A method of detecting an analyte is provided. The method includes providing a sample, a container 110 with a wall 115, and a catalyst for a luminescent reaction. The wall includes a colored portion 115b. The method further comprises forming a reaction in the container and detecting the presence or absence of light emitted from the reaction mixture in the container. Detecting light emitted from the container can comprise detecting light passing through the colored portion. The colored portion can be detected visually and the color can be associated with the identity of an analyte—specific reagent disposed in the container. Kits comprising the container and a catalyst for a luminescent reaction are also provided.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: October 7, 2014
    Assignee: 3M Innovative Properties Company
    Inventors: Neil Percy, Gregory W. Sitton
  • Detection of multiple nucleic acid sequences in a reaction cartridge
    Patent number: 8852863
    Abstract: The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
    Type: Grant
    Filed: May 4, 2010
    Date of Patent: October 7, 2014
    Assignee: Qiagen GmbH
    Inventors: Thomas Rothmann, Holger Engel, Ralf Himmelreich, Andy Wende, Rainer Dahlke
  • Sequences and their use for detection and characterization of O157:H7
    Patent number: 8846349
    Abstract: This invention relates to a rapid method for detection and characterization of Escherichia coli bacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect E. coli O157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: September 30, 2014
    Assignee: E.I. du Pont de Nemours and Company
    Inventor: Frank R. Burns
  • System and method including analytical units
    Patent number: 8840848
    Abstract: Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: September 23, 2014
    Assignee: Beckman Coulter, Inc.
    Inventor: Charles S. Kraihanzel
  • DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
    Patent number: 8835110
    Abstract: The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.
    Type: Grant
    Filed: November 4, 2009
    Date of Patent: September 16, 2014
    Assignee: The Johns Hopkins University
    Inventors: Tza-Huei Wang, Kelvin J. Liu, Ie-Ming Shih
  • Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier
    Patent number: 8822670
    Abstract: A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
    Type: Grant
    Filed: August 9, 2006
    Date of Patent: September 2, 2014
    Assignees: Sumitomo Bakelite Company, Ltd., Kenji Kinoshita
    Inventors: Kenji Kinoshita, Toru Yakabe, Kentaro Fujimoto, Kanehisa Yokoyama, Kazuhiko Fujiwara
  • Method for identifying olfactory receptor included in one olfactory cell
    Patent number: 8808987
    Abstract: The present invention provides a novel method for identifying an olfactory receptor included in one olfactory cell. In the present invention, amplified is the cDNA derived from the mRNA of the one olfactory cell by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02. Subsequently, determined is whether or not a gene sequence of the amplified cDNA is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A. Finally, determined is that, if the gene sequence of the cDNA is identical to the one gene sequence in the previous step, the olfactory receptor included in the one olfactory cell is the olfactory receptor corresponding to the one gene sequence which is identical to the gene sequence of the cDNA in the previous step.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: August 19, 2014
    Assignee: Panasonic Corporation
    Inventor: Masato Suzuki
  • Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers
    Patent number: 8809518
    Abstract: The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides of the invention may be constituted by said linker or population of linkers and a target first strand polynucleotide bound to said linker. The invention also encompasses a method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. The linkers or polynucleotide-linkers of the invention can be used in a method of preparing a cDNA library.
    Type: Grant
    Filed: October 4, 2010
    Date of Patent: August 19, 2014
    Assignee: Riken
    Inventor: Yoshihide Hayashizaki
  • Sequences and their use for detection and characterization of STEC bacteria
    Patent number: 8802371
    Abstract: This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: August 12, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Stephen Varkey, Daniel R. DeMarco, Mark A. Jensen
  • Method of elongating DNA through immobilizing primer DNA chains on a substrate, a method of amplifying a DNA chain
    Patent number: 8765417
    Abstract: Primers for DNA elongation are immobilized onto a substrate having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphoester composing the hydrophilic section of a phospholipid, and a second unit having an activated ester group, and DNA elongation reaction is allowed to proceed in a reaction system having introduced therein a sample which contains DNA fragments having desired sequences and nucleotide monomers, by heating the reaction system up to a temperature causing thermal unfolding of the DNA chains, and then by cooling the reaction system down to a temperature for annealing.
    Type: Grant
    Filed: November 29, 2005
    Date of Patent: July 1, 2014
    Assignees: Sumitomo Bakelite Co., Ltd., DNA Chip Research Inc.
    Inventor: Kenji Kinoshita