Abstract: The present invention relates to novel inducible genes from alfalfa. These genes are highly induced following mechanical harvesting or wounding of alfalfa. The genes are useful for inducible production of heterologous proteins following harvesting.

Abstract: The abundance of the 96 most abundant EST cluster sequences in a wheat lemma/palea cDNA library was investigated in a range of cDNA libraries made from various wheat tissues. 30 cDNA sequences showing highly enhanced abundance in lemma, palea and glume tissues over leaf, stem, embryo, endosperm and root tissue were selected for further analysis. These wheat EST cluster sequences were used to identify rice cDNA homologs. The abundance of the rice cDNA homologs was compared in rice leaf and panicle (includes lemma and palea) cDNA libraries. Rice cDNAs showing preferential expression in the panicle were then used to identify homologous rice genomic DNA clones, the putative promoter sequences have been identified and cloned.

Abstract: The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.

Abstract: The invention provides methods and compositions for the production of transgenic plants, plant cells, embryos, and seeds that produce plants with floral organs that have increased mass and/or size as compared to wild-type plants. The transgenic plants can be transformed with a genetic construct comprising an ipt gene under the control of an APETAL3 promoter.

Abstract: The present invention relates to promoters related to the CEP1 promoter from Zea mays that are useful for regulating gene expression of heterologous polynucleotide molecules in plants. The invention also relates to expression constructs and transgenic plants containing the heterologous polynucleotide molecules.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The invention provides a method of producing a recombinant polypeptide having ?-glucosidase enzymatic activity, a polynucleotide encoding the polypeptide, nucleic acid constructs carrying the polynucleotide, transformed cells, such as yeast cells, and transgenic organisms expressing the polynucleotide. The invention provides various uses of the polypeptide, the polynucleotide, cells and/or organisms, including producing a recombinant polypeptide having ?-glucosidase enzymatic activity, increasing the level of aromatic compounds in alcoholic beverages, as well as other fermentation products of plant material, hydrolyzing cellobiose and thus increasing the level of fermentable glucose, increasing the production of alcohol; such as ethanol from plant material, increasing the aroma released from a plant, or a plant, product, and hydrolysis or transglycosylation of glycosides.

Abstract: The present invention provides recombinant promoters that drive tissue-specific expression, and transgenes comprising such recombinant promoters. Specifically, the invention provides transgenes comprising a recombinant promoter that drives tissue-specific expression of a heterologous nucleic acid molecule in a floral organ. The invention also provides methods for using such transgenes to produce a protein in a plant host cell or transgenic plant. The invention further provides methods for producing a transgenic plant that produces, for example, longer-lasting flowers, better fragrance, or better or longer-lasting color as compared to a wild type plant.

Abstract: The present invention pertains to novel cocoa polypeptides having a molecular weight of about 10 and 14 kDa and being derived from a 69 kDa precursor. In particular, the present invention relates to the production of the polypeptides via recombinant means and the use of the polypeptides or fragments thereof for the production of cocoa/chocolate flavor.

Abstract: Disclosed herein are novel DNA constructs encoding dihydrodipicolinic acid synthase that are useful for generating transgenic events. Also provided are transgenic plants harboring such DNA constructs, the expression of which results in increased lysine in the plant or plant product. Also provided are maize plants having LY038 Event and progeny thereof having this event. Additionally disclosed are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site.

Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.

Abstract: An isolated solely pollen-specific promoter derived from Arabidopsis comprise a nucleotide sequence of Arabidopsis PLA2-? gene. The novel promoter has specific roles in particular organs and at particular developmental stages, such as in mature flowers, and can be used in the production of hybrid seeds using male sterility techniques.

Abstract: The current invention provides the promoter of the Zea mays nuclear gene encoding glutamine synthetase. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the cytoplasmic glutamine synthetase promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The present invention provides novel anther-specific genes, their promoters, and uses of the same. Rice seedlings were treated with gibberellin, and then genes and proteins with up-regulated expression were screened using DNA microarray analysis and proteome analysis. As a result, eight types of ?-tubulins were identified. Among them, OsTUB8 was expressed specifically in the anther. Thus OsTUB8 was suggested to be involved in male sterility in rice, and it was thought that regulating OsTUB8 expression in plants could alter plant fertility. In addition, OsTUB8 promoters were thought to comprise anther-specific activity. Thus, the present invention can be said to be highly valuable when used as a tool for anther-specific gene expression.

Abstract: The inventions is drawn towards vectors and methods useful for preparing genetically transformed plant cells that express immunogens from pathogenic organisms which are used to produce immunoprotective particles useful in vaccine preparations. The invention includes plant optimized genes that encode the HN protein of Newcastle Disease Virus. The invention also relates to methods of producing an antigen in a transgenic plant.

Abstract: The invention includes a method of transposing a non-autonomous transposon gene of rice (Oryza sativa). The method comprises culturing anthers of rice or treating roots, seeds, leaves, and stems of axillary buds of rice, or a callus derived from them, with 5-azacytidine or 5-azadeoxycytidine. Also included in the invention is a plant or plant seeds produced by the method.

Abstract: Provided is a nucleotide sequence of a promoter from a gene encoding a 55 kDa maize prolamin family protein. Also provided are methods that utilize the 55 kDa maize prolamin family gene promoter to express heterologous sequences in plants, expression cassettes that include the 55 kDa maize prolamin family gene promoter, and plants transformed with expression cassettes that include the 55 kDa maize prolamin family gene promoter.

Abstract: The present invention is in the field of plant genetics and biochemistry. More specifically, the present invention relates to genes and polypeptides associated with the tocopherol biosynthesis pathway, namely those encoding homogentisate prenyl transferase activity, and uses thereof. In particular, the sequence of the HPT2 gene from Arabidopsis thaliana is disclosed for expression in any plant species to increase the levels of tocopherol.

Abstract: The invention provides isolated polynucleotides and their encoded proteins that are involved in splicing or modulating splicing activity. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering splicing protein content and/or composition of plants.

Abstract: This invention relates to plants and plant cells which express heterologous D-amino acid metabolizing enzymes and may therefore employ D-amino acids as a source of nitrogen. Methods and means are provided for selectively modulating the growth and stress tolerance of such plants using D-amino acids. The methods can be used either for detoxification of phytotoxic D-amino acids such as D-alanine and D-serine, thereby allowing for selection of plants comprising said D-amino acid metabolizing enzymes, or for enhancing toxicity of lesser phytotoxic D-amino acids such as D-isoleucine, thereby allowing for selection of plants not comprising said D-amino acid metabolizing enzymes.