Patents Examined by Bao-Thuy Nguyen
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Patent number: 9244065Abstract: Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.Type: GrantFiled: March 16, 2012Date of Patent: January 26, 2016Assignee: Sandia CorporationInventors: Ulrich Y. Schaff, Gregory J. Sommer, Anup K. Singh
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Patent number: 9229000Abstract: The present invention relates to a system and a method for the immunological detection of several substances or a substance having several features in one step, the detection reaction being based on an immunological sandwich type method and being in particular characterized in that it can easily be carry out and is designed as a “one-pot method”, which means that all detection steps are carried out in one reaction batch without any wash steps and with only one addition of reagents.Type: GrantFiled: November 3, 2008Date of Patent: January 5, 2016Assignee: ZENTERIS GMBHInventors: Friedrich Menges, Jenny Zahringer
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Patent number: 6303389Abstract: The invention includes assay cassettes that can be employed during rapid flow-through binding assays. The assay cassettes can be disposable units suitable for one-time use and readily assembled to include a filter membrane carried between an upper plate and a lower plate. A pattern of channels can extend through the top plate to allow a fluid sample to be applied through the top plate and onto the filter. The bottom plate can include a plurality of channels that are aligned with the channel of the top plate and which will allow a negative pressure to be applied to the underside of the filter membrane to draw the sample through the filter. In one embodiment, the cassette includes a frangible section that allows the cassette to be divided into a first and second component.Type: GrantFiled: June 29, 1998Date of Patent: October 16, 2001Assignee: ImmuneticsInventors: Andrew E. Levin, William Bowers
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Patent number: 6221655Abstract: This invention relates to a spin filter assembly and its usage to facilitate the isolation and analyses of molecules and compounds such as proteins present in various biological and non-biological solutions. The spin filter assembly of this invention comprise of a tube having an open top end, a bottom end opposite the open top end, a body of a first length, an inner diameter and a first outer diameter, a filter means at the bottom end, the tube insertable to an upper portion of a centrifuge holder having a closed lower end, an open upper end, a length longer than the first length of the tube, and a second inner diameter greater than the first outer diameter of the tube; and, a container closure means that fits on top of the open end of the tube. The spin filter assembly can be a component of a sample preparation or assay kit, for example, a kit for analyzing proteins by immunoprecipitation. A process for analyzing proteins by immunoprecipitation using the spin filter assembly is described.Type: GrantFiled: August 1, 1998Date of Patent: April 24, 2001Assignee: CytosignalInventors: Bernard Kwok-Keung Fung, Sohail Jahid
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Patent number: 6159745Abstract: A test device for detecting or quantifying determining an analyte in a test solution includes an absorbent material having contact, liposome lysing and electrochemical measurement portions. The contact portion is positioned for contact with and uptake of the test solution. The liposome lysing portion is segregated from the contact portion and has a liposome lysing agent bound thereto. The liposome lysing portion is further either positioned between the contact portion and the electrochemical measurement portion, or partially or completely coincides with the electrochemical measurement portion. The electrochemical measurement portion comprises a first conductor comprising a plurality of fingers disposed on the absorbent material, and a second conductor similarly comprising a plurality of fingers disposed on the absorbent material, where the fingers of the first and second conductors are interdigitated to form an array.Type: GrantFiled: July 15, 1999Date of Patent: December 12, 2000Assignees: Cornell Research Foundation, Inc., Innovative Biotechnologies, Inc.Inventors: Matthew A. Roberts, Richard Allen Durst, Richard A. Montagna, Geoffrey S. Rule
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Patent number: 6143576Abstract: The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include, flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.Type: GrantFiled: March 3, 1997Date of Patent: November 7, 2000Assignee: Biosite Diagnostics, Inc.Inventor: Kenneth F. Buechler
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Patent number: 6001659Abstract: Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for a enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.Type: GrantFiled: May 9, 1995Date of Patent: December 14, 1999Assignee: Abbott LaboratoriesInventors: Nai-Yi Wang, Roger C. Hu
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Patent number: 5965378Abstract: The invention concerns a composition composed of several different antibodies or/and antibody fragments which is suitable as a reagent to reduce interferences in an immunological method for the class-specific detection of antibodies from one or several of the immunoglobulin classes G, M, A, D and E.Type: GrantFiled: April 24, 1997Date of Patent: October 12, 1999Assignee: Roche Diagnostics GmbHInventors: Dittmar Schlieper, Helmut Lenz, Urban Schmitt, Volker Klemt
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Patent number: 5866432Abstract: The present invention is related to a rapid and reliable method for making chair-side diagnoses of periodontal diseases and especially for predicting the risk of progressing of the periodontal diseases. The diagnosis and prognosis are performed using an immunological test kit by which increased levels of neutrophil gelatinase-related lipocalin (NGAL) derived from polymorphonuclear neutrophilic leucocytes PMNs) is measured.Type: GrantFiled: December 11, 1996Date of Patent: February 2, 1999Assignee: Oy Medix Biochemica ABInventors: Timo Arto Sorsa, Sari Hannele Tikanoja, Leila Christina Lundqvist
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Patent number: 5702910Abstract: The present invention relates to a sandwich immunoassay for rapidly and readily measuring N-peptide using two kinds of monoclonal antibodies recognizing different portions of the N-peptide. The method for measuring N-peptide or a precursor thereof includes the steps of: incubating a mixture containing a sample and a first monoclonal antibody recognizing a portion of N-peptide; adding a labelled second monoclonal antibody recognizing a portion of N-peptide to the mixture, followed by further incubation; and detecting the resulting antigen-antibody complex in the mixture. Alternatively, the method includes the steps of: incubating a mixture containing a sample, a first monoclonal antibody recognizing a portion of N-peptide, and a labelled second monoclonal antibody recognizing another portion of N-peptide; and detecting the resulting antigen-antibody complex.Type: GrantFiled: December 8, 1995Date of Patent: December 30, 1997Assignee: Shionogi & Co., Ltd.Inventors: Yoshito Numata, Hidehisa Asada, Keiji Dohi, Takahiro Fukunaga, Yasushi Taniguchi