Patents Examined by Blondel Hall
  • Fused polypeptides and methods for their detection
    Patent number: 4880911
    Abstract: Fused-Polypeptides comprising a polypeptide product and a covalently bonded charged amino acid polymer are disclosed. Methods of detecting the presence of the charged amino acid polymer are used to quantitate the presence of the fused-polypeptides either in crude or purified solutions. The charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide product. Examples using polyarginine and carboxypeptidase B to produce urogastrone and gamma interferon are included.
    Type: Grant
    Filed: May 9, 1985
    Date of Patent: November 14, 1989
    Assignee: G. D. Searle & Co.
    Inventors: Stephen J. Brewer, Helmut M. Sassenfeld