Abstract: An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.

Abstract: A WCH-1 cDNA probe specific to mRNA expressing a water channel localized in the kidney collecting tubule and complementary to said mRNA, and the sequence has been identified. The same is obtained bya) subjecting a single-chain cDNA prepared from kidney medullary mRNA of a mammal (rat) to PCR using, as degenerate primers, 5'- (T/C)T(T/C/A/G)AA(T/C)CC(T/C/A/G)GC(T/C/A/G)GT (T/C/A/G)AC- 3' (SEQ ID NO:1) and 5'- AA(T/C/A/G)(G/C)(T/A)(T/C/A/G)C(G/T)(T/C/A/G)GC(T/C/A/G) GG(A/G)TT-3' (SEQ ID NO:2), andb) screening a kidney cDNA library of said mammal using a product of said PCR as a probe. WCH-1 protein molecules constituting said water channel can be produced by Escherichia coli producing protein molecules expressed by WCH-1 gene.

Abstract: The present invention relates to the PUR protein, nucleotide sequences and expression vectors encoding PUR, and to methods for inhibiting PUR activity. Inhibitors of PUR activity may be used to treat hyperproliferative diseases such as cancer.

Abstract: A vector and a prokaryote transformed therewith which includes nucleic acid sequences which make possible the autocatalytic deletion of nucleotide sequences encoding an antibiotic resistance phenotype. The prokaryote can be a bacterium, and in particular a mycobacterium. Such transformed mycobacteria may be employed in vaccines, thereby eliminating the attendant risk of vaccines including antibiotic resistance markers.

Abstract: A multifunctional RNA having self-processing activity, the preparation thereof and the use thereof.Host cells can be transformed so that they express ribozyme RNA and antisense RNA which are connected with each other via a spacer. The RNA molecules can, for example, be complementary to a certain viral RNA. Plants which have been transformed with genes coding for RNA of this type show a significantly improved resistance to viruses.

Abstract: A hybrid vector produced from a linearized cosmid and arms from .lambda. phage vector DNA has been developed. The hybrid vector can be used to generate fragments which are useful as vectors in a helper phage-mediated transformation system, permitting large fragments of foreign DNA to be introduced into a host on an industrial scale.

Abstract: Serial analysis of gene expression, SAGE, a method for the rapid quantitative and qualitative analysis of transcripts is provided. Short defined sequence tags corresponding to expressed genes are isolated and analyzed. Sequencing of over 1,000 defined tags in a short period of time (e.g., hours) reveals a gene expression pattern characteristic of the function of a cell or tissue. Moreover, SAGE is useful as a gene discovery tool for the identification and isolation of novel sequence tags corresponding to novel transcripts and genes.

Abstract: The present invention relates to the PUR protein, nucleotide sequences and expression vectors encoding PUR, and to methods for inhibiting PUR activity. Inhibitors of PUR activity may be used to treat hyperproliferative diseases such as cancer.

Abstract: The invention provides methods for expressing foreign genes in enteric protozoa. This transfection system was established using a gene ligated to the 5' and 3' flanking DNA regions of a protein-encoding gene from an enteric protozoa. The present invention also provides such transformed enteric protozoa, vaccines produced therefrom and foreign or altered proteins expressed in the same. The ability to introduce and express genes in amebae will now permit both genetic analysis and modification of the virulence of this organism, which remains a serious threat to world health and will facilitate basic research towards the control of this parasite.

Abstract: A WCH-1 cDNA probe specific to mRNA expressing a water channel localized in the kidney collecting tubule and complementary to said mRNA, and the sequence has been identified. The same is obtained bya) subjecting a single-chain cDNA prepared from kidney medullary mRNA of a mammal (rat) to PCR using, as degenerate primers, 5'-(T/C)T(T/C/A/G)AA(T/C)CC(T/C/A/G)GC(T/C/A/G)GT (T/C/A/G)AC-3' (SEQ ID NO:1) and 5'- AA(T/C/A/G)(G/C)(T/A)(T/C/A/G)C(G/T)(T/C/A/G)GC(T/C/A/G) GG(A/G)TT-3' (SEQ ID NO:2), andb) screening a kidney cDNA library of said mammal using a product of said PCR as a probe. WCH-1 protein molecules constituting said water channel can be produced by Escherichia coli producing protein molecules expressed by WCH-1 gene.

Abstract: Reagents useful for the rapid processing of synthetic oligonucleotides, including a basic reagent for the cleavage and deprotection of the synthesized oligonucleotides from a support, and a precipitating reagent useful for recovering the synthesized oligonucleotide by precipitation from solution. The basic reagent optionally includes a wetting agent useful for the cleavage from lipophilic supports.

Abstract: Disclosed is a human GABA.sub.A epsilon subunit receptor and DNA (RNA) encoding such polypeptides (RNA). Also provided is a procedure for producing such polypeptides by recombinant techniques and agonists and antagonists for such polypeptides. Also provided are methods of using the agonists, for example, to treat anxiety, Huntington's Chorea, muscular spasms and rigidity, and sleep and seizure disorders. Antagonists may be used, for example, to diagnose and treat anxiety, Huntington's Chorea, sleep and seizure disorders, Alzheimer's disease, Parkinson's disease and overdoses with benzodiazepine and for enhancing cognition and reversing sedation after application of general anesthesia during surgery. Also disclosed are diagnostic methods for detecting mutations in the polynucleotides of the present invention and for detecting levels of the soluble polypeptides in samples derived from a host.

Abstract: The present invention relates to methods of cleaving phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences can be used to facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.

Abstract: A 2 .mu.m plasmid vector for transforming yeast, especially brewing yeast, much which comprises a DNA sequence allowing propagation of the plasmid in bacteria, two copies of the 74 base pair FLP recombination site of the 2 .mu.m plasmid in direct orientation and a DNA sequence coding for a protein or peptide of interest. The plasmid is so constructed that in yeast the bacterial DNA sequence is between the two recombination sites which are in direct orientation and is spontaneously lost following recombination. A "gene of interest" is preferable inserted at the SnaBI site.

Abstract: Efficient integration of heterologous DNA into yeast genomic DNA is accomplished at high copy number by targeting integration vectors to dispersed repetitive elements such as DELTA sequences, Ty elements, or tRNA DNA sequences present in the host cell genome.

Abstract: A food-grade vector is provided which is suitable for transforming a food-grade host cell and is incapable of replicating autonomously in the host cell due to deletion of the replicase gene.

Abstract: A recombinant DNA molecule coding for a protein having the activity of an interferon regulatory factor-1 (IRF-1).

Abstract: Oligosacaharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.

Abstract: Origin of Replication Complex (ORC) genes, nucleic acids which encode ORC proteins and hybridization reagents, probes and primers capable of hybridizing with ORC genes and methods for screening chemical libraries for lead compounds for pharmacological agents useful in the diagnosis or treatment of disease associated undesirable cell growth are provided. An exemplary screen involves forming a mixture comprising a recombinant ORC protein, a natural intracellular ORC protein binding target, and a candidate pharmacological agent; incubating the mixture under conditions whereby, but for the presence of said candidate pharmacological agent, said ORC protein selectively binds said binding target; and detecting the presence or absence of specific binding of said ORC protein to said binding target.

Abstract: A method of inhibiting the growth of bFGF-dependent neoplastic cells is disclosed. The method includes the steps of accessing a selected colony of such cells and adding a bFGF-specific antisense primer to such colony. By performing the adding step, there is downregulating of the expression of colony-intracellular bound bFGF, and by performing the downregulating step, there is inhibiting of bFGF-promoted growth of cells in the colony.