Abstract: An apparatus for the large scale culturing and packaging of cell suspensions, three dimensional tissue, and other biological systems is disclosed. The apparatus includes a plurality of flexible or semi-flexible treatment chambers comprising one or more individual culture pockets, a plurality of rigid spacers, an inlet fluid manifold, an outlet fluid manifold, a fluid reservoir, and a means for transporting fluid within the system. During treatment, liquid media is transported from the fluid reservoir to the inlet manifold, which will in turn evenly distribute the media to each of the connected treatment chambers and internal culture pockets. An outlet fluid manifold is also provided to ensure that each treatment chamber is evenly filled and to ensure that any air bubbles formed during treatment are removed from the treatment chambers. The treatment chambers are flexible or semi-flexible so as to provide for easy end-user handling during rinsing and application of the cultured transplants.

Abstract: Disclosed are a method for testing for the presence of a cytomegalovirus antigen by using an antibody which specifically recognizes cytomegalovirus Lower matrix protein pp65, as a primary antibody, which comprises using an alkaline phosphatase-labeled antibody as a secondary antibody and a method for testing for the presence of a cytomegalovirus antigen which comprises using an alkaline phosphatase-labeled antibody which specifically recognizes cytomegalovirus Lower matrix protein pp65, or (Fab').sub.2 or Fab' fragment of the antibody which specifically recognizes cytomegalovirus Lower matrix protein pp65 as a primary antibody.

Abstract: An immunoscintillating composition and method of radioimmunoassay is disclosed whereby polymer coated paramagnetic particles and binding agent such as an antibody are associated by chemical or physical means to provide solid scintillating immunoadsorbent particles which are paramagnetic. These particles are capable of selectively binding or retaining radioactive or labeled antigens or ligands and transmitting radioactive energy to a phosphor or photon emitting substance that is bound by chemical or physical means to the particles. Upon binding of the antigens or ligands to the surface of the particles the particles are separated from the unbound components in solution by application of a magnetic field. The luminescence emitted by the phosphor which is attached to the magnetically separated particles is measured by a scintillator counter and is directly proportional to the radioactive energy released by the labeled antigen bound to the antibody.

Abstract: The present invention is directed to a process of isolating immunoglobulins from whey or whey concentrate and a concentrated immunoglobulin product which is highly purified. The process features the co-precipitation of lipids and non-immunoglobulin proteins simultaneously with a charged polymer and a fatty acid.

Abstract: An apparatus for cleaning of a reactor pressure vessel head closure stud. The surface of the stud is cleaned by power vacuum brushing. The stud is hung vertically in a cabinet which supports a vertically elevatable spinner housing. The stud is cleaned in a single process using a rotating wire brush system which is rotatably mounted in the spinner housing and has a central opening for passage of the stud. A plurality of wire bushes are arranged in a circular array surrounding the stud. While the wire brushes are rotating in contact with the stud external surface, the spinner housing and wire brush assembly are displaced vertically by an elevator so that the rotating brushes travel along the full length of the stud. The rotating brushes remove accumulated material on the stud surface. The resulting contaminated debris is contained in a volume enclosed by a brush canister and a bellows. The bellows expands or contracts as the elevator travels.

Abstract: A novel method of disrupting cells which do not have a cell wall comprises passing suspended cells through a low pressure impinging jet device. This method disrupts the cells, but does not harm the cell products which are liberated.

Abstract: In this invention a novel antigen is provided for screening patients suspected of having celiac disease. The antigen is prepared by starting with human placenta tissue which is perfused with a herpes and collagenase buffer to obtain a signle cell suspension. This suspension is enriched to obtain an enriched protein portion and then separated out to obtain an embryonic celiac antigen (ECA). This ECA is used with serum from patients to effect binding of ECA with IgA in the serum while applying a human IgA antibody to the serum. The results are then read on a spectrophotometer to confirm or negate the presence of celiac disease.