Patents Examined by Brian Lathrop
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Patent number: 6037145Abstract: A process for the production of a desired polypeptide comprising the steps of: (1) transforming host cells with an expression vector comprising a gene coding for a fusion protein comprising a desired polypeptide and a protective polypeptide; (2) culturing the transformed host cells so as to express said gene to produce a fusion protein; and (3) excising the desired polypeptide from the fusion protein with a protease intrinsic to the host cells. According to the present invention, a large amount of a desired polypeptide can be produced at a low cost. Especially according to the present invention, a large amount of S. aureus V8 protease can be efficiently produced at low cost using a safe host such as E. coli according to gene recombination procedures.Type: GrantFiled: September 5, 1995Date of Patent: March 14, 2000Assignee: Suntory LimitedInventors: Masayuki Yabuta, Kazuhiro Ohsuye
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Patent number: 5922590Abstract: Polynucleotide and polypeptide sequences encoding a novel tumor suppressor, HIC-1 , are provided. Also included is a method for detecting a cell proliferative disorder associated with HIC-1. HIC-1 is a marker which can be used diagnostically, prognostically and therapeutically over the course of such disorders.Type: GrantFiled: May 25, 1995Date of Patent: July 13, 1999Assignee: The Johns Hopkins University School of MedicineInventors: Stephen B. Baylin, Michele Makos Wales
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Patent number: 5919900Abstract: Compositions enriched for Neutrophil Inhibitory Factor which inhibit neutrophil activity including adhesion to vascular endothelial cells are provided. Also provided are recombinant Neutrophil Inhibitory Factors which also which inhibit neutrophil activity. Such compositions may comprise a glycoprotein isolated from nematodes. These compositions and recombinant Neutrophil Inhibitory Factors are useful in the therapy of conditions which involve abnormal or undesired inflammatory responses.Type: GrantFiled: May 26, 1995Date of Patent: July 6, 1999Assignee: Corvas International, Inc.Inventors: Matthew Moyle, David L. Foster
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Patent number: 5861377Abstract: The present invention relates to novel inhibitors belonging to the family of antistasin-type serine proteinase inhibitors, to their isolation from the medical leech Hirudo medicinalis, to DNA sequences encoding the novel inhibitors, to variants obtained by recombinant DNA technology or peptide synthesis, pharmaceutical compositions containing the inhibitors, and to their use in diagnosis and therapy.Type: GrantFiled: January 6, 1995Date of Patent: January 19, 1999Assignee: Novartis AGInventors: Hans Fritz, Christian Sommerhoff, Jutta Heim
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Patent number: 5858705Abstract: A human DNA Ligase III polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptide via gene therapy for the treatment of disorders associated with a defect in DNA Ligase III. Antagonists against such polypeptides and their use as a therapeutic to destroy unwanted cells are also disclosed. Diagnostic assays to detect mutant DNA Ligase III genes are also disclosed.Type: GrantFiled: June 5, 1995Date of Patent: January 12, 1999Assignee: Human Genome Sciences, Inc.Inventors: Ying-Fei Wei, Guo-Liang Yu, William A. Haseltine
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Patent number: 5855889Abstract: A purified and isolated DNA sequence and the encoded mammary-specific protein, mammaglobin, are disclosed. Also disclosed are methods for the detecting breast cancer based upon the overexpression and secretion of mammaglobin by breast cancer cells. The methods detect and/or quantitate the presence of mammaglobin or the mRNA encoding mammaglobin.Type: GrantFiled: September 30, 1996Date of Patent: January 5, 1999Assignee: Washington UniversityInventors: Mark A. Watson, Timothy P. Fleming
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Patent number: 5852169Abstract: Disclosed are substatially pure preparations of Max-Interacting (Mxi) polypeptides, DNA encoding such polypeptides, antibodies recognizing such polypeptides, and diagnostic and therapeutic methods utilizing such polypeptides.Type: GrantFiled: June 5, 1995Date of Patent: December 22, 1998Assignee: The General Hospital CorporationInventors: Roger Brent, Antonis S. Zervos
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Patent number: 5849536Abstract: The subject invention provides non-glycosylated, biologically active polypeptides which comprise the vWF (von Willebrand Factor) GP1b binding domain. These polypeptides may be used to inhibit platelet adhesion and aggregation in the treatment of subjects with conditions such as cerebrovascular disorders and cardiovascular disorders. This invention also provides expression plasmids encoding these polypeptides as well as methods of producing by transforming a bacterial cell and recovering such polypeptides. In addition, the subject invention provides methods of treating and preventing cerebrovascular, cardiovascular and other disorders using these polypeptides to inhibit platelet aggregation.Type: GrantFiled: November 30, 1994Date of Patent: December 15, 1998Assignee: Bio-Technology General Corp.Inventors: Leonard Garfinkel, Tamar Richter
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Patent number: 5837499Abstract: Disclosed are polypeptide analogues of human C5a which are C5a receptor antagonists that exhibit substantially no analphylatoxin or agonist activity, and derivatives of the analogues, and dimeric forms of the analogues or derivatives. DNA molecules encoding the polypeptides and methods of making the analogues are also provided. Pharmaceutical formulations containing a C5a analogue, are used therapeutically in the treatment of C5a-mediated diseases and inflammatory conditions in mammals, and prophylactically to prevent or reduce inflammation caused by an event which causes inflammation or aggravates an existing inflammatory condition, respectively. Further disclosed are antibodies specific to the C5a analogues, derivatives thereof, and dimers of the analogues and derivatives which exhibit substantially no cross-reactivity with human C5a. The antibodies are used to detect or quantify circulating C5a analogue or derivative, as well as to modify, e.g.Type: GrantFiled: June 5, 1995Date of Patent: November 17, 1998Assignee: Ciba-Geigy CorporationInventors: Jan van Oostrum, William C. Boyar, Nicholas G. Galakatos, Albert Schmitz, Gino Van Heeke
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Patent number: 5830659Abstract: A method and system for separating a selected molecule from a heterogeneous mixture of molecules in aqueous solution are described. The method comprises (a) providing a separation device comprising a loading reservoir and a receiving reservoir coupled by a channel bearing immobilized microtubules aligned parallel to the longitudinal axis thereof the channel; (b) placing an aqueous solution containing the heterogeneous mixture of molecules in the loading reservoir; (c) adding a motor-ligand composition and ATP to the aqueous solution, wherein the motor-ligand composition comprises a motor protein for attaching to microtubules and moving therealong in the presence of ATP and the ligand is capable of binding the selected molecule, such that the ligand binds the selected molecule to form a complex and the complex moves along the immobilized microtubules to the receiving reservoir; and (d) removing the selected molecule from the receiving chamber.Type: GrantFiled: September 13, 1996Date of Patent: November 3, 1998Assignee: University of Utah Research FoundationInventor: Russell J. Stewart
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Patent number: 5830700Abstract: Hybrid proteins having cross-linking and tissue-binding activities, DNA molecules encoding such proteins and methods for producing the hybrid proteins from recombinant host cells are disclosed. The hybrid proteins disclosed herein are useful in tissue sealant and wound healing formulations.Type: GrantFiled: November 1, 1995Date of Patent: November 3, 1998Inventor: Meher Irani
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Patent number: 5821107Abstract: The present invention relates to the use of these cyclophilins, hereinafter referred to as "cyclophilin-like proteins (CLP)" in a method for identifying compounds capable of binding to and/or inhibiting the enzymatic activity of these proteins. Such compounds may be further screened for their ability to inhibit parasites which are not susceptible to the anti-parasitic effects of CsA.Type: GrantFiled: May 26, 1995Date of Patent: October 13, 1998Assignee: New England Biolabs, Inc.Inventors: Clotilde K. S. Carlow, Antony Page
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Patent number: 5821082Abstract: A domain of Bcl-2 that suppresses apoptosis by allowing cell survival permits cell proliferation when mutated. The wild type domain includes amino acid residues 51 to 97 (SEQ ID NO:13) of Bcl-2. Peptides including the domain and nucleotides encoding the domain are useful in molecular screening of human tumors for the presence of mutations that allow proliferation of cells that were otherwise marked for apoptosis. The peptides are also useful to screen for proteins that play a role in the modulation of cellular proliferation.Type: GrantFiled: May 23, 1996Date of Patent: October 13, 1998Assignee: St. Louis University Health Sciences CenterInventor: Govindaswamy Chinnadurai
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Patent number: 5821047Abstract: A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.Type: GrantFiled: June 5, 1995Date of Patent: October 13, 1998Assignee: Genentech, Inc.Inventors: Lisa J. Garrard, Dennis J. Henner, Steven Bass, Ronald Greene, Henry B. Lowman, James A. Wells, David J. Matthews
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Patent number: 5814603Abstract: PTH analogs comprising an amino acid sequence that is: SVSEIQLLHNX.sub.1 X.sub.2 X.sub.3 HX.sub.4 X.sub.3 X.sub.3 X.sub.3 X.sub.5 RVX.sub.5 WLR X.sub.4 X.sub.4 LX.sub.3 X.sub.3 VX.sub.1 X.sub.3 X.sub.3 X (SEQ ID NO:10) wherein X.sub.1 is a neutral or positively charged amino acid, X.sub.2 is a neutral amino acid, X.sub.3 is a neutral, positively charged, or negatively charged amino acid, X.sub.4 is a positively charged amino acid, X.sub.5 is a positively charged or negatively charged amino acid, and X is selected from the group consisting of Hol, Ho, a homoserine amide, or the sequence of amino acids comprising residues 35-84 of PTH, have enhanced activity and increased serum half-life as compared with human PTH. The PTH analogs can be produced as fusion proteins in high yields in E. coli host cells; the fusion proteins can be subsequently cleaved to produce the PTH analog.Type: GrantFiled: October 25, 1993Date of Patent: September 29, 1998Assignee: Affymax Technologies N.V.Inventors: Kevin R. Oldenburg, Harold E. Selick
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Patent number: 5807818Abstract: The present invention provides therapeutic methods for treatment of conditions including the neutralization of the anti-coagulant activity of heparin, inhibition of angiogenesis, tumor and endothelial cell proliferation, and treatment of chronic inflammatory diseases by administration of bactericidal/permeability-increasing (BPI) protein products.Type: GrantFiled: May 5, 1995Date of Patent: September 15, 1998Assignee: Xoma CorporationInventor: Roger G. Little, II
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Patent number: 5798223Abstract: A Human amine transporter polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also provided are methods for detecting agonists and antagonists to such polypeptide and the use of agonists and antagonists for treating diseases related to the underexpression and over-expression of the Human amine transporter of the present invention. Also disclosed are methods for detecting mutations in the nucleic acid sequence encoding the polypeptide and for detecting altered levels of the soluble form of the polypeptide.Type: GrantFiled: June 6, 1995Date of Patent: August 25, 1998Assignee: Human Genome Sciences, Inc.Inventors: Yi Li, Liang Cao, Craig A. Rosen
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Patent number: 5792638Abstract: The oncogene of the present invention, isolated by expression cloning from a human ovarian cancer is a mutant of TC21. The present invention teaches that ras-related genes not thought to have transforming potential can contribute importantly to cancers which have been refractory to oncogene detection. The present invention teaches that another ras relative gene, R-ras, which was previously reported to lack transforming potential, has transforming capacity as well. Thus, these and other genes similarly related to prototype and activated by analogous mechanisms may be important in the diagnosis and prognosis of certain cancers, as well as be critical in the design of rational approaches to therapy of cancers in which they play a role.Type: GrantFiled: May 24, 1994Date of Patent: August 11, 1998Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Stuart A. Aaronson, Andrew Chan, Toru Miki
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Patent number: 5789199Abstract: A process is provided for producing a heterologous polypeptide in bacteria. This process comprises, in a first step, culturing bacterial cells that lack their native pstS gene and comprise nucleic acid encoding a PstS variant having an amino acid variation within the phosphate-binding region of the corresponding native PstS, nucleic acid encoding a DsbA or DsbC protein, nucleic acid encoding the heterologous polypeptide, a signal sequence for secretion of both the DsbA or DsbC protein and the heterologous polypeptide, an inducible promoter for the nucleic acid encoding the DsbA or DsbC protein, and an alkaline phosphatase promoter for the nucleic acid encoding the heterologous polypeptide. The nucleic acid encoding a PstS variant is under the transcriptional control of the wild-type pstS gene promoter. The second step of the process involves recovering the heterologous polypeptide from the periplasm or the culture medium.Type: GrantFiled: October 31, 1996Date of Patent: August 4, 1998Assignee: Genentech, Inc.Inventors: John C. Joly, James R. Swartz
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Patent number: 5783416Abstract: A human spasmolytic polypeptide (HSP) having the amino acid of SEQ ID NO:1, characterized by being in a glycosylated form.Type: GrantFiled: August 2, 1995Date of Patent: July 21, 1998Assignee: Novo Nordisk A/SInventors: Lars Thim, Kjeld Norris, Fanny Norris, S.o slashed.ren Erik Bj.o slashed.rn, Mogens Christensen, Per Franklin Nielsen