Abstract: Disclosed herein are methods and compositions comprising chimeric green fluorescent protein (GFP) molecules embedded with foreign molecules. Also disclosed are methods of treating a subject with chimeric GFP molecules embedded with foreign molecules. Further disclosed are methods of delivering a desired molecule to a cell, wherein said desired molecule is embedded within a chimeric GFP molecule.

Abstract: The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.

Abstract: The present invention falls within the technical field of aquaculture, and specifically, the invention relates to a specific solution for preventing and treating bacterial diseases using a Lactococcus lactis lactic acid bacterium transformed to produce an interferon type II (IFN II) immunostimulating cytokine, particularly interferon gamma (IFNg or IFNy). Said transformed bacterium has been deposited in the Chilean Microbial Genetic Resources Collection at INIA with accession number RGM 2416 dated 22 Oct. 2017.

Abstract: The object is to provide a method for inducing pili formation in bacterium of genus Bifidobacterium and a method for promoting intestinal colonization of the bacterium. 3-Phenylpropionic acid and/or 3-(4-hydroxyphenyl)propionic acid are an active ingredient of a composition for inducing the pili formation in the bacterium of genus Bifidobacterium and a composition for promoting the intestinal colonization of the bacterium.

Abstract: The present disclosure relates to a method of digesting a sample comprising protein, the method comprising: adding the sample to an apparatus containing a buffer and a solid support surface comprising a surface coating, wherein the surface coating immobilizes enzyme while reducing undesired interactions between the sample and the solid support surface; immobilizing enzymes on the surface coating for digestion of the protein of the sample; and heating the sample to complete a heat-assisted digestion of the protein.

Abstract: Disclosed herein is a composition for modulating immunity, comprising Lactobacillus paracasei LT12, ?-glucan, and Bovine Colostrum Powder. Also provided is a method for modulating immunity, comprising administering to a subject in need a therapeutically effective amount of the composition, wherein the method is to enhance the expression of IL-6, IL-10, TNF-? and TGF-?1.

Abstract: The present specification discloses methods and uses for treating a migraine disorder. The disclosed method comprising extramuscularly administering a Botulinum toxin to an individual in one of more areas of a fronto-fascial layer located in a frontal head region of the individual, one of more areas of a temporoparietal-fascial layer located in a temporal head region of the individual, and one or more areas of an occipito-fascial layer located in an occipital head region of the individual. The disclosed methods and uses further comprise extramuscularly administering a Botulinum toxin to one or more nerve exit points and/or one or more subcutaneous locations in the head and neck as well as one or more sites of an epicranial aponeurosis. The disclosed methods and uses further comprise intramuscularly administering a Botulinum toxin to one or more locations within the left and right Splenius Capitus muscle, the left and right Masseter muscles, the left and right Trapezius muscles, or any combination thereof.

Abstract: Disclosed herein is a method for assessing the probability of embryo implantation success during the in vitro fertilization process. A method of the disclosure can predict embryo implantation success based on, for example, the amount of specific metabolites present in embryo conditioned culture media.

Abstract: The present invention is related to a trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 80% identical to and/or at least 80% homologous to an amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are selected from the group consisting of amino acid positions of SEQ ID NO: 1712, 383, 10, 114, 118, 192, 197, 220, 225, 304, 306, 318, 323, 339, 349, 357, 459, 476, 481, 484, 487, 488, 506, 511, 526, 530, 532, 533, 537, 550, 556, 564, 590, 649, 667, 703 and 705.

Abstract: The present application provides methods and systems for identifying host cell protein impurities which have enzymatic activities in biopharmaceutical products and in samples during manufacturing processes. The present application provides various activity-based probes to characterize different enzyme classes of host cell protein impurities including probes containing functionalized molecules with reporter or affinity-based tags.

Abstract: The present invention relates to a microorganism with increased carbon monoxide availability and use thereof.

Abstract: Lactiplantibacillus plantarum TCI837 is provided, deposited in the Leibniz Institute DSMZ under an accession number of DSM 33843. Based on this, a method for improving gut microbiota in a subject in need thereof is also provided, comprising administering to the subject a composition comprising an effective dose of Lactiplantibacillus plantarum TCI837, wherein improving the gut microbiota comprises increasing probiotics and reducing non-probiotics.

Abstract: The present invention includes light-controlled and light-independent neurotoxin systems and methods for using such neurotoxin systems for rapidly and locally silencing distinct populations of neurons.

Abstract: The present invention discloses a cyclohexenecarboxylate ester hydrolase, and a mutant, a coding gene, an expression vector, recombinant bacterium and use thereof. The cyclohexenecarboxylate ester hydrolase AcEst1 and its mutant of the present invention have the function of enantioselectively resolving methyl 3-cyclohexene-1-carboxylate with high efficiency to prepare optically active (S)-3-cyclohexene-1-carboxylic acid. When the substrate concentration is as high as 2000 mM (about 280 g/L), the optical purity of the product is higher than 99%, and the substrate/catalyst is as high as 3500 g/g. As compared with other preparation methods, the product prepared by the method of the present invention has high concentration and high optical purity, the catalytic efficiency is high, the reaction conditions are mild. Furthermore, the method is environmentally friendly, simple in operation and easy for industrial scale-up, thus has a good prospect of application in industry.

Abstract: A lactic acid bacterium is provided which has high foodstuff suitability, easy to manufacture, and can increase the blood iron concentration, thereby improving anemia. A lactic acid bacterium of salt-tolerant that increases the blood iron concentration.

Abstract: Isolated Rhodococcus ruber, a product derived from the Rhodococcus ruber (in particular, a product derived from the Rhodococcus ruber cell wall), a method for preparing the product derived from the Rhodococcus ruber, and use of the product derived from the Rhodococcus ruber in prevention and/or treatment of lichen planus. The product derived from the Rhodococcus ruber effectively reduce the erosion area of lichen planus and relieve pain.

Abstract: Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.

Abstract: The present invention relates to an inert carrier Salmonella and potential use thereof, which is expected to be developed into a new inert carrier bacteria, and can be applied to the development of an indirect agglutination test method for simple and rapid detection of antigens or infected antibodies. The inert carrier Salmonella has been deposited in CGMCC in Beijing on Mar. 18, 2019 with the accession number of CGMCC No.17340, and is classified as Salmonella sp.with a strain code of S9. The Salmonella has no visible agglutination reaction with various chicken sera derived from different genetic backgrounds, i.e., it has no non-specific agglutination reaction with chicken sera derived from broad range of genetic backgrounds.

Abstract: Methods and compositions are provided for preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas axonopodis in a plant. The invention provides novel bacteriophages virulent to Xylella fastidiosa or Xanthomonas axonopodis, including XfaMija and XfaMijo, and further provides methods for treating or preventing Pierce's Disease or Citrus Canker in plants.

Abstract: This disclosure relates to a magnetic mixing apparatus that mixes a sample contained in a mixing chamber using a stir bar, while minimizing the amount of contact between the stir bar and walls of the mixing chamber. In one aspect, the apparatus comprises a ferromagnetic stir bar contained in the mixing chamber, and a driving magnet and a driven magnet located on opposite sides of the mixing chamber. The driving magnet, the driven magnet, and the ferromagnetic stir bar are each capable of rotating about a respective axis. The driving magnet, the driven magnet, and the ferromagnetic stir bar are magnetically coupled such that rotation of the driving magnet induces rotation of the driven magnet and rotation of the driving magnet and the driven magnet induce rotation of the ferromagnetic stir bar. In some embodiments, rotation of the ferromagnetic stir bar within the mixing chamber mixes the sample contained within the mixing chamber.