Abstract: Antigenic rare outer membrane proteins of Spirochaetaceae are obtained from organisms extracted from infected tissue by a novel process of isolation utilizing a discontinuous Ficoll gradient separation, release of outer membrane in a low isotonic and low pH buffer and identification of outer membrane by use of a lipid soluble dye. Four antigenic rare outer membrane proteins of T. pallidum subsp. pallidum useful in diagnosis and prophylaxis of syphilis are provided. Also provided is the amino acid sequence of a rare outer membrane protein of T. pailidum subsp. pallidum and the nucleotide sequence encoding it.
Type:
Grant
Filed:
April 23, 1997
Date of Patent:
October 13, 1998
Assignee:
The Regents of the University of California
Inventors:
David R. Blanco, James N. Miller, Michael A. Lovett, Cheryl I. Champion
Abstract: A genetic mutation within the SR calcium release channel provides a test for susceptibility to idiopathic dilated cardiomyopathy. The test detects the presence of the mutation in a sample of nucleic acids obtained from the individual being tested. Restriction fragment length polymorphism is one technique which can be used in the test.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
June 17, 1997
Assignee:
The Regents of the University of California
Inventors:
Luyi Sen, Kenneth D. Philipson, Aldons Jake Lusis