Patents Examined by Carol E. Bidwell
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Patent number: 5147779Abstract: A method for evaluating the potential immunogenicity of a protein derived from recombinant DNA technology. The method involves injecting an animal with the recombinant protein and then isolating antiserum from the animal. The antiserum is depleted of antibodies to a reference protein, i.e., a plasma derived protein, by adsorbing the antiserum against the reference protein. The adsorbed antiserum is then blotted against the recombinant protein, to see if any antibodies were produced which recognize the recombinant protein, but did not recognize the plasma-derived protein during adsorption.Type: GrantFiled: June 1, 1988Date of Patent: September 15, 1992Assignee: Miles Inc.Inventors: Pamela C. Esmon, Michael A. Fournel
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Patent number: 5145773Abstract: This invention provides a method of detecting sensitivity to alpha-interferon therapy which comprises contacting a sample with a monoclonal antibody under conditions so as to form an antibody-antigen complex, detecting the complex so formed, and thereby detecting sensitivity to alpha-interferon.Type: GrantFiled: May 25, 1989Date of Patent: September 8, 1992Assignee: Sloan-Kettering Institute for Cancer ResearchInventors: Anthony P. Albino, David M. Nanus, Neil H. Bander, Lawrence M. Pfeffer
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Patent number: 5143826Abstract: The present invention relates to a method to detect feline blood type B comprising: combining feline blood with a sufficient amount of Triticum vulgaris lectin to agglutinate feline blood type B. Additionally, this invention relates to a kit to detect feline blood type B.Type: GrantFiled: November 9, 1990Date of Patent: September 1, 1992Assignee: Kansas State Univ. Research FoundationInventor: Joseph E. Smith
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Patent number: 5143825Abstract: A 4-methylumbelliferyl phosphate substrate for use in conducting an immunoassay is disclosed. The substrate contains 0.1 gram mole per liter 2-amino-2-methyl-1-propanol, 0.01 to 0.05 gram mole per liter EDTA, added as trisodium EDTA, 1 gram per liter sodium azide, 1.2 milligram mole per liter 4-methylumbelliferyl phosphate and 4 milligram moles per liter tetramisole. The pH of the substrate disclosed is 10.3.Type: GrantFiled: March 1, 1990Date of Patent: September 1, 1992Assignee: Abbott LaboratoriesInventors: Koshy T. Chacko, Karen L. Fitzgerald, James J. Markese
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Patent number: 5141876Abstract: The invention refers to a method as well as a composition for the quantitative determination of kidney damages by the application of polyclonal and monoclonal antibodies against villin and/or fimbrin appearing in the urine as soon as damages of the kidney, for example reactions of rejection in case of a transplanted kidney or tubular inflammations, are occurring.Type: GrantFiled: February 21, 1989Date of Patent: August 25, 1992Inventor: Detlef Drenckhahn
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Patent number: 5139932Abstract: A method for the diagnosis of IgA nephropathy using a specific binding reaction comprising the steps:a) preparing a substrate capable of binding fibronectin or IgAb) contacting the substrate resulting from step a) with a sample of body fluid drawn from a patient subject to diagnosis to bind any fibronectin-IgA-complex present in said sample to the substrate, andc) determining the presence of complex bound to the substrate using the reaction between the exposed part of such bound complex and a corresponding antibody thereto; anda diagnostic kit for use in such diagnosis.Type: GrantFiled: November 3, 1989Date of Patent: August 18, 1992Assignee: BioCarb ABInventors: Bo Cederholm, Jorgen Wieslander, Per Bygren, Dick Heinegard
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Patent number: 5139933Abstract: An assay method is provided to quickly detect the presence of Listeria strains in samples, characterized by the use of antibodies to selectively capture the peptidoglycan and teichoic acid components of the listeriae bacterial cell wall.Type: GrantFiled: June 25, 1990Date of Patent: August 18, 1992Assignee: VICAM, L.P.Inventors: Calvert L. Green, Franz Fiedler, Thomsen J. Hansen, Gerald N. Wogan, Steven R. Tannenbaum, Thomas L. Benjamin
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Patent number: 5135875Abstract: A precipitation reagent for use in analytical systems for the determination of hydrophobic analytes in a biological test sample, particularly analytical systems employing specific binding proteins for such analytes. The precipitation reagent precipitates interfering proteins, hemoglobin, and other interfering substances from a biological test sample while, at the same, maintaining hydrophobic analytes in solution and minimizing the denaturation of specific binding proteins, such as, for example, antibodies, which may be present in an immunoassay system. The precipitation reagent comprises a zinc salt, a glycol, and a straight or branced alcohol from about 1 to 4 carbon atoms, and may optionally contain an acid. A preferred precipitation reagent comprises zinc sulfate, methanol and ethylene glycol, and is particularly useful in a fluorescent polarization immunoassay for the determination of hydrophobic analytes, especially cyclosporine.Type: GrantFiled: August 15, 1990Date of Patent: August 4, 1992Assignee: Abbott LaboratoriesInventors: Victoria P. Meucci, Elizabeth A. Simpson, Mariola B. Zajac
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Patent number: 5135874Abstract: A method for evaluating the potential immunogenicity of a protein derived from recombinant DNA technology. The method involves injecting an animal with the recombinant protein and then isolating antiserum from the animal. The antiserum is depleted of antibodies to a reference protein, i.e., a plasma derived protein, by adsorbing the antiserum against the reference protein. The adsorbed antiserum is then blotted against the recombinant protein, to see if any antibodies were produced which recognize the recombinant protein, but did not recognize the plasma-derived protein during adsorption.Type: GrantFiled: March 15, 1990Date of Patent: August 4, 1992Assignee: Miles Inc.Inventors: Pamela C. Esmon, Michael A. Fournel
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Patent number: 5134075Abstract: The present invention is concerned with a novel monoclonal antibody which binds strongly to a protein antigen associated with human tumors, including carcinomas of the colon, breast, ovary and lung, as well as melanomas and sarcomas. The antibody binds to normal human cells to a much lesser degree than to tumor cells. The antibody finds use both in diagnostic methods such as the detection of malignant cells associated with tumors and in therapeutic methods for treatment of humans with tumors. Also disclosed is a novel 100,000 dalton glycoprotein antigen found on the cell surface of human tumor cells. The amino terminal amino acid sequence of this antigen is: ##STR1## in which X represents an unidentified amino acid.Type: GrantFiled: February 17, 1989Date of Patent: July 28, 1992Assignee: Oncogen Limited PartnershipInventors: Karl E. Hellstrom, Ingegerd Hellstrom, Hans Marquardt, Yoshitaka Yoneyama
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Patent number: 5132210Abstract: The present invention relates to (1) an enzyme-linked immunosorbent assay (ELISA) for detection in milk of antibodies of any isotype which are specific for Staphylococcus aureus proteins in molecular weights ranging from 14,000 to 26,000 daltons, (2) a process for production and purification of the proteins, (3) a method of performing the ELISA utilizing the proteins (4) use of the ELISA for detection of intramammary infection by S. aureus, (5) preparation of monoclonal and polyclonal antibodies against the selected fractions, (6) use of such antibodies of purification of infection specfic antigens, and (7) use of the antibodies in an ELISA for antibodies produced in an individual in response to infection by S. aureus.Type: GrantFiled: June 12, 1989Date of Patent: July 21, 1992Assignee: ProScience CorporationInventors: D. Scott Adams, Travis C. McGuire, Jr.
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Patent number: 5128270Abstract: The invention relates to assays of a monokine in a biological fluid, in particular in a blood sample, in particular of TNF or of IL-1. According to the present invention, the monokine in the serum or plasma obtained from whole blood stimulated with a mitogenic agent is assayed prior to any separation between the plasma (or serum) and the blood cells. According to another subject of the invention, the monokine is assayed in the case of an immunometric assay employing an oligoclonal system, consisting of several different monoclonal antibodies adsorbed on a solid phase and a non-adsorbed labeled antibody.The invention also relates to immunoassay kits.Type: GrantFiled: January 6, 1989Date of Patent: July 7, 1992Assignee: Ire-Medgenix S.A.Inventors: Dominique Delacroix, Donat De Groote, Paul Franchimont, Philippe Gysen, Aimee Reuter, Isabelle Dehart
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Patent number: 5126244Abstract: A method for the liberation of polysaccharide antigens from bacteria or fungi in biological probes or in grown cultures of these probes is described. In the majority of cases such a liberation is necessary in order that subsequently the polysaccharide antigens can be determined immunologically in a manner known per se. This liberation is effected by treating the biological probe or the grown culture with an aqueous phenol solution. A 1-10% phenol solution is conveniently used, with a 2.5% phenol solution being preferred and a 2% phenol solution being especially preferred. The phenol solution is allowed to act on the probe or culture for 5-30%, preferably 15, minutes. No extraction is necessary for the subsequent immunological determination of the polysaccharides.Type: GrantFiled: November 17, 1988Date of Patent: June 30, 1992Assignee: Hoffmann-La Roche Inc.Inventor: Hanne-Lene Muller
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Patent number: 5124250Abstract: An element for assaying a rheumatoid factor quantitatively in biosamples, comprising a polyalkyl methacrylate solid carrier and serum albumin immobilized thereon, the serum albumin being immunologically bound with anti-albumin rabbit IgG, and a method of assaying a rheumatoid factor quantitatively by immunoglobulin class in which the element is reacted with the biosample, then the element-bound rheumatoid factor is reacted with enzyme-labeled anti-human IgG, enzyme-labeled anti-human IgM or enzyme-labeled anti-human IgA, and then the amount of the marker enzyme is determined.Type: GrantFiled: September 11, 1989Date of Patent: June 23, 1992Assignee: Toyo Boseki Kabushiki KaishaInventors: Mami Inada, Hakuji Matsumoto, Tsuneo Hanyu, Kyoichi Kano
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Patent number: 5124245Abstract: An aqueous wash solution is useful for the detection of herpes simplex virus in a biological specimen. This solution has a pH of from about 9 to about 11.5, and consists essentially of an alcoholamine or a salt thereof and a nonionic surfactant. The solution is used to wash uncomplexed materials from a complex of herpes simplex antigen and antibodies thereto. The wash solution can be supplied as part of a diagnostic test kit.Type: GrantFiled: February 9, 1989Date of Patent: June 23, 1992Assignee: Eastman Kodak CompanyInventors: Thomas J. Cummins, Sheryl S. Sullivan
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Patent number: 5122447Abstract: A method of testing serum from swine vaccinated against pseudorabies virus with viral envelope-based subunit vaccines to determine the presence of antibodies to infecting pseudorabies virus in wihch an immunoassay is performed on the swine serum using a pseudorabies virus antigen preparation comprising nucleocapsid proteins of the pseudorabies virus. The universal diagnostic antigen is one or more nucleocapsid proteins having relative molecular weights of approximately 23 k, 34 k, 41 k, 63 k and 140 k.Type: GrantFiled: January 23, 1989Date of Patent: June 16, 1992Assignee: Iowa State University Research Foundation, Inc.Inventors: Michael J. McGinley, Kenneth B. Platt
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Patent number: 5114845Abstract: The present invention relates to an improved method for measuring soluble fibrin utilizing a genetically modified tissue plasminogen activator protein as a substrate.Type: GrantFiled: August 9, 1989Date of Patent: May 19, 1992Assignee: Biopool International, Inc.Inventor: Mats G. Ranby
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Patent number: 5093270Abstract: A method for releasing encapsulated marker molecules from within liposomes comprises contracting the liposomes with an effective amount of a water-miscible alcohol. The chemiluminescence of acridinium esters is enhanced by oxidizing the acridinium ester in the presence of an effective amount of a water-miscible alcohol.Type: GrantFiled: May 24, 1989Date of Patent: March 3, 1992Assignee: Ciba Corning Diagnostics Corp.Inventors: Steve C. S. Chang, Say-Jong Law
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Patent number: 5091306Abstract: The invention provides a method for testing for the presence of catalase in a milk sample comprising combining a milk sample with a substantially catalase-free alkaline protease enzyme enriched detergent to disrupt catalase containing somatic cells present therein and release active catalase therefrom in the present of a pH buffering salt having a concentration of at least 20 mM in the resulting sample solution and providing the solution with a pH in a range of about 7.0-8.0 and thereafter testing the sample for the presence of catalase, as well as providing a test kit for detecting the presence of catalase in milk comprising a substantially catalase-free alkaline protease enzyme enriched detergent and 20 to 400 micromoles of a pH buffer alone or in combination with any other solute capable of reducing the solubility of oxygen in milk and of providing the resulting milk solution with a pH in the range of about 7.0-8.0.Type: GrantFiled: March 2, 1988Date of Patent: February 25, 1992Assignee: Yissum Research Development Company of the Hebrew University of JerusalemInventor: Nathan Citri
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Patent number: 5089389Abstract: A buffered composition includes whole chlamydial or gonococcal organisms or an antigen thereof, and a nonionic fluorinated surfactant in an amount of at least about 0.05 weigh percent. This composition is useful in a positive control test used in diagnostic assays for chlamydial or gonococcal organisms. It is particularly useful when coated in an article used in such assays, such as disposable test devices having nonionic polyamide filtration membranes in the bottom of test wells.Type: GrantFiled: August 25, 1989Date of Patent: February 18, 1992Assignee: Eastman Kodak CompanyInventors: Geraldine A. Pelanek, Robert W. Zercie, James D. Kanaley