Patents Examined by Carolyn L Greene
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Patent number: 11970736Abstract: The present disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the disclosed embodiments is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The disclosed embodiments includes two target-specific nucleic acid probes per genetic target, a barcode loop oligo and a bridge oligo or bridge oligo complex.Type: GrantFiled: August 31, 2022Date of Patent: April 30, 2024Assignee: Genomill Health OyInventors: Juha-Pekka Pursiheimo, Tatu Hirvonen, Anttoni Korkiakoski, Manu Tamminen
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Patent number: 11939630Abstract: Disclosed is a fluorescent PCR method for detecting HLA-B*15:02 allele and a specific primer probe combination. In the present disclosure, a set of primers and probes are designed based on an HLA-B*15:02 specific SNP gene locus by using TaqMan probe technology, combining another set of primers and probes corresponding to the internal reference gene ?-Actin, and a set of primer probe for non-HLA-B*15:02 genes are designed to detect whether a DNA sample contains an HLA-B*15:02 gene and whether a sample is homozygous or heterozygous. Compared with the similar detection methods in the past, the technical scheme in the present disclosure inherits the advantages of high specificity, high throughput, high resolution, low cost, simple and convenient operation, process controllability and the like of the fluorescent PCR, and may detect whether a sample is homozygous or heterozygous.Type: GrantFiled: August 19, 2021Date of Patent: March 26, 2024Assignee: Shaanxi Lifegen Co., Ltd.Inventors: Penggao Dai, Zihua Zhong, Hao Wang, Zhiye Cai, Lei Meng, Le Wang
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Patent number: 11932905Abstract: Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.Type: GrantFiled: March 17, 2023Date of Patent: March 19, 2024Assignee: Singular Genomics Systems, Inc.Inventors: Ryan Shultzaberger, Allen Lipson, Daan Witters
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Patent number: 11905554Abstract: A sequencing primer set comprising a universal upstream primer, a universal downstream primer and a downstream primer in rich of promoter; wherein the universal upstream primer has a sequence of: 5?-TBarcodeCAAAXXXXNNN-3?; the universal downstream primer has a sequence of: 5?-GACTGCGTACGZZZZNNN-3?(SEQ ID No. 9); the downstream primer in rich of promoter has a sequence of: 5?-GACTGCGTACYYNCTATA-3?(SEQ ID No. 7).Type: GrantFiled: September 25, 2020Date of Patent: February 20, 2024Inventors: Zhiqiang Xia, Meiling Zou, Wenquan Wang
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Patent number: 11898199Abstract: The present disclosure provides, among other things, methods of screening for colorectal cancer, methods of screening for advanced adenoma, methods of screening for the presence of either colorectal cancer or advanced adenoma (or both), and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer and/or advanced adenoma screening that includes analysis of methylation status of one or more methylation biomarkers, and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer and/or advanced adenoma screening that include screening methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA. In various embodiments, the present disclosure provides methods for colorectal cancer and/or advanced adenoma screening that include screening methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA, using MSRE-qPCR.Type: GrantFiled: November 11, 2019Date of Patent: February 13, 2024Assignee: Universal Diagnostics, S.A.Inventors: Marko Bitenc, Kristi Kruusmaa, Juan Martinez-Barea, Christian Hense, Marko Chersicola, Primo{hacek over (z)} Knap
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Patent number: 11884981Abstract: One aspect of the invention is a method for amplifying alpha globin genes HBA1, HBA2 and HBA12 in a single PCR tube to determine an HBA genotype of a subject. This method employs five primers selected to accurate and sensitively identify the HBA1, HBA2, and HBA12, a gene found at a higher frequency in citizens of Saudi Arabia, by accurately annealing to nucleic acids in a biological sample and simultaneously amplifying sequences encoding the alpha globin genes. This invention includes a procedure and required reagents for the amplification of alpha globin genes in a single PCR tube.Type: GrantFiled: May 27, 2021Date of Patent: January 30, 2024Assignee: Imam Abdulrahman Bin Faisal UniversityInventors: J. Francis Borgio, Sayed Abdulazeez, Fahd A. Al-Muhanna, Amein Kadhem Al-Ali
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Patent number: 11873535Abstract: Described herein are methods and kits for authentication of botanical DNA fragments isolated from processed botanical products, including dietary supplements or nutraceutical compositions including botanical extracts.Type: GrantFiled: July 27, 2018Date of Patent: January 16, 2024Assignee: HERBALIFE INTERNATIONAL OF AMERICA, INC.Inventors: Zhengfei Lu, Silva Babajanian, Yanjun Zhang, Peter Chang, Gary Swanson, Maria Rubinsky
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Patent number: 11859244Abstract: Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.Type: GrantFiled: March 17, 2023Date of Patent: January 2, 2024Assignee: Singular Genomics Systems, Inc.Inventors: Ryan Shultzaberger, Allen Lipson, Daan Witters
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Patent number: 11827926Abstract: Described herein are methods and compositions related to the diagnosis and treatment of COVID 19 and the detection of SARS-Cov-2.Type: GrantFiled: March 23, 2021Date of Patent: November 28, 2023Inventors: Constance L. Cepko, Brian Anthony Rabe
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Patent number: 11827944Abstract: Provided herein is an antibiotic susceptibility test and related compositions, methods and systems based on detection of a nucleic acid from a target microorganism in a sample in the presence or absence of a lysis treatment of the sample.Type: GrantFiled: October 11, 2018Date of Patent: November 28, 2023Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Rustem F. Ismagilov, Emily S. Savela, Nathan Schoepp, Eric J. Liaw
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Patent number: 11821028Abstract: The present disclosure provides methods, sets of substantially complementary double-stranded adapters, and kits for performing nucleic acid sequencing. The substantial complementary double-stranded adapters comprise fully complementary molecular tag regions but one or more mismatches in other regions. Such adapters are ligated to double-stranded target nucleic acids, the obtained ligation products are amplified, and the generated amplification products are sequenced. The methods according to the present disclosure allow both strands of double-stranded target nucleic acids to be sequenced from one end of the target nucleic acids.Type: GrantFiled: July 11, 2017Date of Patent: November 21, 2023Assignee: QIAGEN Sciences, LLPInventor: Yexun Wang
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Patent number: 11821040Abstract: The invention provides in vitro methods of determining whether an individual has a pre-cancer or cancer comprising determining the presence or absence of one or more methylation markers of a methylation marker set in a urine sample of said individual; and determining whether the individual has pre-cancer or cancer based on the detection of the presence or absence of said one or more methylation markers in the urine sample, wherein the presence of said one or more methylation markers indicates that the individual has pre-cancer or cancer. The invention further provides methods for typing pre-cancer or cancer based on the the presence or absence of one or more methylation markers of a methylation marker set in a urine sample.Type: GrantFiled: March 31, 2017Date of Patent: November 21, 2023Assignee: Nanomed Diagnostics BVInventors: Willem Frederik Rurup, Loes Irene Segerink, Roderik Adriaan Kraaijenhagen, Herbert Michael Pinedo, Albert Van Den Berg, Renske Daniëla Maria Steenbergen, Jacobus Adrianus Nieuwenhuijzen, Idris Bahce, Geert Kazemier
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Patent number: 11802307Abstract: Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.Type: GrantFiled: March 17, 2023Date of Patent: October 31, 2023Assignee: Singular Genomics Systems, Inc.Inventors: Ryan Shultzaberger, Allen Lipson, Daan Witters
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Patent number: 11795514Abstract: The invention relates to an isothermal-based dual functional oligonucleotide containing quencher and reporter dye for an isothermal nucleic acid amplification and a method for nucleic acid amplification and measurement using the same. The present invention is directed to a method capable of obviating the need for an additional oligonucleotide, in addition to four to six types of oligonucleotides for a nucleic acid amplification reaction of LAMP, detecting the amount of fluorescence according to the amplification of the nucleic acid of target gene—specific sequence for DNA and RNA, enabling the detection also after the completion of the reaction, and detecting the amount of fluorescence in real-time. Therefore, the present invention allows simultaneous multiple tests by measuring the amount of fluorescence in one tube after the completion of the reaction or in real time while varying the reporter dye according to the target gene.Type: GrantFiled: November 30, 2016Date of Patent: October 24, 2023Assignee: SD BIOSENSOR, INC.Inventors: Yoo Deok Won, Hae Joon Park, Hyo Jin Seong, Mi Ae Lim, Sun Young Lee
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Patent number: 11788119Abstract: Multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence are described. The target sequence is either part of an analyte or indicative of the presence of an analyte in the sample. Each padlock probe includes an analyte-specific reporter sequence, and either a restriction cleavage site located 3? of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction. Where the padlock probe includes a restriction cleavage site, cleavage at the restriction cleavage site occurs 3? of the analyte-specific reporter sequence. Where the padlock probe includes a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5? of the analyte-specific reporter sequence. Panels of probes and kits for the same are also described.Type: GrantFiled: December 15, 2017Date of Patent: October 17, 2023Assignee: Q-Linea ABInventors: Camilla Russel, Jenny Goransson, Mats Gullberg
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Patent number: 11788129Abstract: A GAPDH nucleic acid detection kit includes a primer set for detecting GAPDH nucleic acid. The primer set for detecting GAPDH nucleic acid includes a forward inner primer for GAPDH nucleic acids, a forward outer primer for GAPDH nucleic acids, a backward inner primer for GAPDH nucleic acids and a backward outer primer for GAPDH nucleic acids. The primer set for detecting GAPDH nucleic acid is used in a loop-mediated isothermal amplification (LAMP).Type: GrantFiled: December 10, 2021Date of Patent: October 17, 2023Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Min-Yuan Chou, Kuang-Chi Cheng, Ming-Hua Yang, Jiun-Lin Guo
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Patent number: 11786906Abstract: System, heaters, and heat transfer devices are disclosed. For example, a system for performing polymerase chain reaction includes a support member configured to receive a sample vessel and a heater that is positioned to affect a temperature of the sample vessel. The system additionally includes a heat transfer device disposed between the heater and the sample vessel. The heat transfer device illustratively includes anisotropic fibers axially aligned parallel to one another and positioned to conduct heat from the at least one heater toward the sample vessel in the axial direction of the anisotropic fibers. An exemplary heater includes a body defining one or more channels, a heating element positioned in the one or more channels, and retention members adjacent the one or more channels. At least a portion of the heating element is mechanically interlocked with the channel by deforming the retention members into a closed position.Type: GrantFiled: April 14, 2017Date of Patent: October 17, 2023Assignee: Biofire Defense, LLCInventors: David E. Jones, Michael Bills, Aaron D. Wernerehl, Anson Cole Chamberlain
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Patent number: 11781175Abstract: We propose a method of generating an epigenetic age prediction. The method includes providing a sample extraction test kit, and receiving a sample extracted by the sample extraction test kit. The method further includes extracting DNA from the sample and processing the DNA to receive processed DNA. The method further includes amplifying a plurality of loci in the processed DNA to receive amplified DNA and processing the amplified DNA to receive a plurality of methylation values for one or more CpG sites in the plurality of loci.Type: GrantFiled: June 2, 2022Date of Patent: October 10, 2023Assignee: H42, INC.Inventors: Sandra Ann R Steyaert, Geert Trooskens, Wim Maria R. Van Criekinge, Adriaan Verhelle, Johan Irma H. Vandersmissen
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Patent number: 11783918Abstract: Techniques for random access of particular DNA strands from a mixture of DNA strands are described. DNA strands that encode pieces of the same digital file are labeled with the same identification sequence. The identification sequence is used to selectively separate DNA strands that contain portions of the same digital file from other DNA strands. A DNA staple positions DNA strands with the identification sequence adjacent to sequencing adaptors. DNA ligase joins the molecules to create a longer molecule with the region encoding the digital file flanked by sequencing adaptors. DNA strands that include sequencing adaptors are sequenced and the sequence data is available for further analysis. DNA strands without the identification sequence are not joined to sequencing adaptors, and thus, are not sequenced. As a result, the sequencing data produced by the DNA sequencer comes from those DNA strands that included the identification sequence.Type: GrantFiled: October 30, 2017Date of Patent: October 10, 2023Assignee: Microsoft Technology Licensing, LLCInventors: Karin Strauss, Yuan-Jyue Chen
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Patent number: 11781169Abstract: One or more enzymes are used to repair damage in synthetic DNA molecules that encode digital information. The enzymes are included in a repair mixture containing one or more of DNA polymerase, DNA ligase, T4 Endonuclease, Endonuclease IV, Endonuclease VIII, and uracil glycosylase. The repair mixture may also contain one or more of a buffering solution, oxidized nicotinamide adenine dinucleotide (NAD+), and deoxyribose nucleoside triphosphates (dNTPs). The synthetic DNA molecules are incubated with the repair mixture for approximately four hours. Use of the repair solution allows recovery of the digital information from damaged DNA molecules.Type: GrantFiled: October 5, 2018Date of Patent: October 10, 2023Assignee: MICROSOFT TECHNOLOGY LICENSING, LLCInventors: Robert N. Grass, Alexander Xavier Christof Kohll, Weida Chen